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Transcript
changing sample order 5-7
changing the module for a sample 5-5
cutting and pasting rows 5-8
fill down function 5-4
GeneScan defaults preferences 4-25
how to fill out
DNA sequencing injection list 6-15 to 6-16
fragment analysis injection list 7-13
making multiple injections 5-5
preferences 4-24 to 4-26
preparing
DNA sequencing 6-15 to 6-17
fragment analysis 7-13 to 7-16
Sequencing defaults preferences 4-24
installing
capillary, on empty gel block 3-15 to 3-19
Cathode electrode 3-24
dye set/primer files 4-18
Internet address
customer training information 1-13
Documents on Demand 1-12
L
Leak Detect error 3-11
leaks
polymer, checking for 3-11 to 3-13
syringe, correcting leaks at 3-12
LEDs (lights)
service LEDs 2-5
status indicators 2-4
loading samples
fragment analysis 7-8
sequencing 6-10
Log file, about 4-11 to 4-12
long-term shutdown 3-44 to 3-45
M
maintenance
autosampler
calibrating, how to 3-5
calibrating, when to 3-4
cleaning, how to 3-4
cleaning, when to 3-4
homing, how to 3-6
homing, when to 3-6
buffer
replacing, when to 3-10
storing 3-10
capillary window
cleaning, how to 3-14
checking for polymer leaks 3-11 to 3-13
electrode
cleaning, how to 3-23
cleaning, when to 3-23
polymer
replacing, when to 3-10
resetting the instrument, when to 3-46
syringes
Index-4
cleaning, how to 3-35
cleaning, when to 3-35
inspecting 3-37
water
replacing, when to 3-10
maintenance, system
See Also computer
Manual Control window 4-6 to 4-7
editing a module 4-14
executing a function 4-7
starting a module 4-7
materials required
DNA sequencing 6-7
fragment analysis 7-5
Matrix column
DNA sequencing 6-12
matrix files 2-20
about 6-24, 7-22
creating DNA matrix from a sample 6-30
creating from matrix standards
DNA sequencing 6-30 to 6-32
fragment analysis 7-25 to 7-29
errors 6-29, 6-31
number of files needed 6-24, 7-22
matrix standard samples, in sample sheet 7-10
matrix standards
checking quality
DNA sequencing 6-29 to 6-32
fragment analysis 7-27 to 7-29
denaturing the matrix standard samples
fragment analysis 7-24
sequencing 6-26
DNA sequencing, part numbers B-4
fragment analysis, part numbers B-5
preparing standard samples
DNA sequencing 6-25
fragment analysis 7-23
mobility files. See dye set/primer files
modules
editing 4-14
installing 4-18
pausing 4-7
selecting
DNA sequencing 6-19
fragment analysis 7-18
starting 4-7
monitoring
raw data 4-8
run of the instrument
DNA sequencing 6-22
fragment analysis 7-20
O
On/Off switch
2-6
P
parameters
voltage and time