Download HaloPlex HS Target Enrichment System Automation Protocol For Ion

Sample Preparation
Step 4. Capture and wash the target DNA
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Step 4. Capture and wash the target DNA
In this step, the circularized target DNA-HaloPlex HS probe hybrids,
containing biotin, are captured on streptavidin beads.
Prepare the NGS Bravo
1 Gently wipe down the Bravo deck with a NucleoClean decontamination
wipe.
2 Place a red insert on Bravo deck position 6.
Prepare the Streptavidin beads source plate
3 Vigorously resuspend the Dynabeads MyOne Streptavidin T1 magnetic
beads on a vortex mixer. The magnetic beads settle during storage.
4 Wash the magnetic beads:
a Transfer 40 µl per sample of the Dynabeads MyOne Streptavidin T1
magnetic bead suspension to a 1.5-ml tube or conical vial, using
volumes provided in Table 15.
Table 15
Volume of Dynabeads MyOne Streptavidin T1 magnetic bead suspension for capture
Reagent
Volume for
1 Library
Volume for
1 Column
Volume for
2 Columns
Volume for
3 Columns
Volume for
4 Columns
Volume for
6 Columns
Volume for
12 Columns
Streptavidin T1
Magnetic Beads
0.04 ml
0.36 ml
0.68 ml
1.0 ml
1.32 ml
1.96 ml
3.92 ml
b Put the vial into a compatible magnetic device for 5 minutes.
c After verifying that the solution has cleared, carefully remove and
discard the supernatant using a pipette.
d Add an equivalent volume of HS Capture Solution (see Table 16) to
the beads and resuspend by pipetting up and down.
Table 16
Volume of Capture Solution used for bead resuspension
Reagent
Volume for
1 Library
Volume for
1 Column
Volume for
2 Columns
Volume for
3 Columns
Volume for
4 Columns
Volume for
6 Columns
Volume for
12 Columns
HS Capture
Solution
0.04 ml
0.36 ml
0.68 ml
1.0 ml
1.32 ml
1.96 ml
3.92 ml
HaloPlex HS Target Enrichment System-ION
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