Download HaloPlex Target Enrichment System

Using the Agilent NGS Bravo for HaloPlex Target Enrichment
Overview of the HaloPlex Target Enrichment Procedure
2
Overview of the HaloPlex Target Enrichment Procedure
Figure 2 summarizes the HaloPlex target enrichment workflow. For each
sample to be sequenced, individual HaloPlex-enriched, indexed libraries
are prepared. Depending on the specific sequencing platform used, up to
96 samples can be pooled and sequenced in a single lane.
Table 7 summarizes how the VWorks automation protocols are integrated
into the HaloPlex workflow. See the Sample Preparation chapter for
complete instructions for use of the VWorks protocols for sample
processing.
Table 7
Overview of VWorks protocols used during the workflow
Workflow Step
VWorks Protocol used for Automation
Digest genomic DNA
Digestion.pro
Hybridize to HaloPlex or ClearSeq
probe and index samples
Hybridization.pro
Capture and amplify enriched DNA
Capture_v1.1.pro
Purify amplified libraries
Purification_v1.1.pro
HaloPlex Target Enrichment System Automation Protocol-ILM
25