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Download InviMag Universal RNA Mini Kit/ KF96
Transcript
II. Extraction steps automatically performed on the KingFisher system Binding of the DNA Automatically sample mixing for 5 min. SNAP separation. SNAP transfer to Washing Plate 1. First Washing Automatically sample mixing for 2 min. SNAP separation. SNAP transfer to Washing Plate 2. Second Washing Automatically sample mixing for 1 min. SNAP separation. SNAP transfer to Washing Plate 3. Third Washing and Drying Automatically sample mixing for 1 min. SNAP separation. Drying the SNAP outsight Washing Plate 3 for 7 minutes. SNAP transfer to Elution Plate. Elution of the DNA Automatically sample mixing for 10 minutes. SNAP separation and removal into Washing Plate_3 (disposal). The extracted DNA can now be transferred into 1.5 ml reaction tubes. Important Notes: After the run, the Elution Plate contains the extracted RNA. Store the RNA under adequate conditions. We recommend to transfer the extracted RNA into an 1.5 ml reaction tube for further storage and freeze the DNA at -20°C. Alternatively, the Elution Plate can be sealed and stored at –80°C. If the extracted RNA contains carryover of magnetic particles, transfer the RNA into a 1.5 ml reaction tube, centrifuge at maximum speed for 1 min and transfer the RNAcontaining supernatant into a new tube. 17 ® InviMag Universal RNA Mini Kit/ KF96 0515
