Download tutorial 1 - Textco BioSoftware

Menu Items
When you initiate the PCR analysis you will see Figure 7.27, page 7-40.
There are a number of parameters that you can define as listed in the Parameters list on the left side of the window. Clicking on any item in the list will
show a description of the item on the right side of the window in the Description field. Once you press the Generate Primers button, GCK will return a
list of possible primer pairs (Figure 2.80, page 2-76). Choose a pair and
press the Show Info button to see details about how the various attributes
of the primer pair scored. Once a pair is selected, you can add it to the construct by pressing Add Primer button. Once you are finished adding the
primers, press the Done button to close the window. Any primers that you
added to the construct are displayed as pairs of opposite facing indicators,
one on the top and one on the bottom of the construct DNA. You can click on
a primer pair, Get Info..., and see the same information that was available in
the Show Info window.
Database Searching
There are many databases available over the Internet which contain information relevant to biologists. For details on how to use the DB searching capabilities, see Tutorial 18:Database Searching, page 2-81. The basic idea is that
you can provide keywords, protein or DNA sequences to GCK and the program will send that information to one or more database sites on the web.
The searching is managed through a DB searching engine on Textco BioSoftware’s web server. The DB server accepts either DNA, protein or keyword
information and sends out queries to various web sites. The DB server contains information on how to format queries for different sites and formats the
query information appropriately for each site. If the site changes how it wants
new queries formatted, the DB server will be updated negating the need to
update individual applications. This arrangement also means that we can
update the DB server with new sites and remove obsolescent sites without
necessitating updating all of the GCK clients.
If you have suggestions for new sites that you feel would be of general interest, please let us know about them by sending us an e-mail at [email protected].
Page 7-41