Download 51400 - Protocol (20 prep)

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Transcript 
Flow Chart
Procedure for Purifying Blood DNA using Norgen’s Blood DNA Isolation Midi Kit
Obtain anticoagulated blood sample and transfer into a tube containing Proteinase K
Add Lysis Buffer B. Vortex.
Spin briefly. Incubate.
SPIN
(optional)
Add Ethanol
Bind to column
SPIN
Wash once with Solution WN.
Wash twice with Wash Solution A
Dry spin.
SPIN
Elute DNA with
Elution Buffer B
SPIN
Pure Genomic DNA
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