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VI. 8-Point Standards After reconstitution of the lyophilized cytokine standard mix, the 8-point cytokine concentration used for generating the standard curve of a given antigen is listed below. The detection sensitivity of each protein in one experiment is user dependent. Try our array specific Quantibody Q-Analyzer to see your Limit of Detection (LOD). (Section VIII). Serial standard concentration (pg/ml) (pg/ml) 4-1BB ACE ALK-1 CT-1 CD27 CD40L CTLA-4 Decorin Dkk-1 Dtk Endoglin Fc RIIB Flt-3L Galectin-1 Galectin-3 Gas 1 Gas 6 GITR L HAI-1 HGF R IL-1 R4 IL-3 R IL-9 JAM-A Leptin R L-Selectin Lymphotactin MadCAM-1 MFG-E8 MIP-3 Neprilysin Pentraxin 3 RAGE TACI TREM-1 TROY TSLP TWEAK R VEGF R1 VEGF R3 Cntrl 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Std7 34 137 14 55 34 55 3 7 55 27 14 14 34 14 3 3 3 1 14 34 55 55 27 7 7 14 274 14 55 1 27 14 34 69 14 5 5 34 14 14 Quantibody Mouse Cytokine Array 6 Std6 103 412 41 165 103 165 10 21 165 82 41 41 103 41 8 8 10 4 41 103 165 165 82 21 21 41 823 41 165 4 82 41 103 206 41 16 16 103 41 41 Std5 309 1,235 123 494 309 494 31 62 494 247 123 123 309 123 25 25 31 12 123 309 494 494 247 62 62 123 2,469 123 494 12 247 123 309 617 123 49 49 309 123 123 14 Std4 926 3,704 370 1,481 926 1,481 93 185 1,481 741 370 370 926 370 74 74 93 37 370 926 1,481 1,481 741 185 185 370 7,407 370 1,481 37 741 370 926 1,852 370 148 148 926 370 370 Std3 2,778 11,111 1,111 4,444 2,778 4,444 278 556 4,444 2,222 1,111 1,111 2,778 1,111 222 222 278 111 1,111 2,778 4,444 4,444 2,222 556 556 1,111 22,222 1,111 4,444 111 2,222 1,111 2,778 5,556 1,111 444 444 2,778 1,111 1,111 Std2 8,333 33,333 3,333 13,333 8,333 13,333 833 1,667 13,333 6,667 3,333 3,333 8,333 3,333 667 667 833 333 3,333 8,333 13,333 13,333 6,667 1,667 1,667 3,333 66,667 3,333 13,333 333 6,667 3,333 8,333 16,667 3,333 1,333 1,333 8,333 3,333 3,333 Std1 25,000 100,000 10,000 40,000 25,000 40,000 2,500 5,000 40,000 20,000 10,000 10,000 25,000 10,000 2,000 2,000 2,500 1,000 10,000 25,000 40,000 40,000 20,000 5,000 5,000 10,000 200,000 10,000 40,000 1,000 20,000 10,000 25,000 50,000 10,000 4,000 4,000 25,000 10,000 10,000