Download Manual

Transcript
VI. 8-Point Standards
After reconstitution of the lyophilized cytokine standard mix, the 8-point
cytokine concentration used for generating the standard curve of a given
antigen is listed below. The detection sensitivity of each protein in one
experiment is user dependent. Try our array specific Quantibody Q-Analyzer
to see your Limit of Detection (LOD). (Section VIII).
Serial standard concentration (pg/ml)
(pg/ml)
4-1BB
ACE
ALK-1
CT-1
CD27
CD40L
CTLA-4
Decorin
Dkk-1
Dtk
Endoglin
Fc RIIB
Flt-3L
Galectin-1
Galectin-3
Gas 1
Gas 6
GITR L
HAI-1
HGF R
IL-1 R4
IL-3 R
IL-9
JAM-A
Leptin R
L-Selectin
Lymphotactin
MadCAM-1
MFG-E8
MIP-3
Neprilysin
Pentraxin 3
RAGE
TACI
TREM-1
TROY
TSLP
TWEAK R
VEGF R1
VEGF R3
Cntrl
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
Std7
34
137
14
55
34
55
3
7
55
27
14
14
34
14
3
3
3
1
14
34
55
55
27
7
7
14
274
14
55
1
27
14
34
69
14
5
5
34
14
14
Quantibody Mouse Cytokine Array 6
Std6
103
412
41
165
103
165
10
21
165
82
41
41
103
41
8
8
10
4
41
103
165
165
82
21
21
41
823
41
165
4
82
41
103
206
41
16
16
103
41
41
Std5
309
1,235
123
494
309
494
31
62
494
247
123
123
309
123
25
25
31
12
123
309
494
494
247
62
62
123
2,469
123
494
12
247
123
309
617
123
49
49
309
123
123
14
Std4
926
3,704
370
1,481
926
1,481
93
185
1,481
741
370
370
926
370
74
74
93
37
370
926
1,481
1,481
741
185
185
370
7,407
370
1,481
37
741
370
926
1,852
370
148
148
926
370
370
Std3
2,778
11,111
1,111
4,444
2,778
4,444
278
556
4,444
2,222
1,111
1,111
2,778
1,111
222
222
278
111
1,111
2,778
4,444
4,444
2,222
556
556
1,111
22,222
1,111
4,444
111
2,222
1,111
2,778
5,556
1,111
444
444
2,778
1,111
1,111
Std2
8,333
33,333
3,333
13,333
8,333
13,333
833
1,667
13,333
6,667
3,333
3,333
8,333
3,333
667
667
833
333
3,333
8,333
13,333
13,333
6,667
1,667
1,667
3,333
66,667
3,333
13,333
333
6,667
3,333
8,333
16,667
3,333
1,333
1,333
8,333
3,333
3,333
Std1
25,000
100,000
10,000
40,000
25,000
40,000
2,500
5,000
40,000
20,000
10,000
10,000
25,000
10,000
2,000
2,000
2,500
1,000
10,000
25,000
40,000
40,000
20,000
5,000
5,000
10,000
200,000
10,000
40,000
1,000
20,000
10,000
25,000
50,000
10,000
4,000
4,000
25,000
10,000
10,000