Download SERVICE Manual Chorus TRIO - DIESSE Diagnostica Senese

Chorus trio – SERVICE MANUAL
CHSMIT200
CHORUS TRIO
SERVICE MENU
VERSION 3.0
Version 3.0 – Revision 0 - 06/04/2011
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Chorus trio – SERVICE MANUAL
CHSMIT30
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Version 3.00, revision 0 of this manual corresponds to the CHORUS TRIO model
of the instrument with the 3.xx family of software installed.
It was drafted and carefully reviewed and this version is closely related to the
instrument model (data can be obtained from the instrument’s ID plate) and the
version of the software that controls it (data can be obtained through a
procedure on the instrument itself).
It must be read carefully before using the instrument, especially the parts
relating to safety.
DIESSE declines all responsibility for improper use of the instrument or failing to
use the instrument as specifically indicated in this manual. The manufacturer’s
responsibility is in any event limited exclusively to the malfunctioning of the
instrument.
Any instrument updating done with the customer’s authorization requires that
the user manual be updated in a corresponding manner. It is the user’s
responsibility to verify that the manual provided corresponds with the version of
the instrument used, with particular regard to the release of the software
installed.
DIESSE Diagnostica Senese SpA declines all responsibility for damage that is
directly or indirectly caused by errors, faults or incidents due to the use of
manuals not corresponding to the version of the supplied instrument.
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TABLE OF CONTENTS
1
MOVING THE STRIPS ................................................................................. 8
1.1
THE WORKSTATIONS ............................................................................................... 8
1.2
THE TRAY ............................................................................................................... 9
1.3
TRAY ROTATION ...................................................................................................... 9
1.3.1
ABSOLUTE SENSOR............................................................................................... 12
1.3.2
STEP CHECK ...................................................................................................... 12
1.3.3
SYNCHRONIZATION ...............................................................................................
13
1.4
STRIP HOUSING .................................................................................................... 14
1.5
TRAY LOCK ........................................................................................................... 15
1.6
CALIBRATIONS AND CONTROL PARAMETERS ............................................................ 16
1.6.1
2
ALIGNMENT AND CENTERING OF THE PLATE. .................................................................. 16
STRIP RECOGNITION UNIT ...................................................................... 17
2.1
STRIP PRESENCE SENSOR (SPS) ............................................................................. 17
2.2
EXTERNAL BARCODE READER ................................................................................. 17
2.3
INTERNAL BARCODE READER ................................................................................. 18
2.4
CALIBRATIONS AND CONTROL PARAMETERS ............................................................ 18
2.4.1
SPS (STRIP PRESENCE SENSOR) .............................................................................. 18
2.4.2
EXTERNAL BARCODE READER .................................................................................... 19
2.4.3
INTERNAL BARCODE READER .................................................................................... 20
3
TRANSFER UNIT ....................................................................................... 20
3.1
DISPENSING HYDRAULIC CIRCUIT .......................................................................... 20
3.2
THE STRIP, NEEDLES AND THE WELL ....................................................................... 21
3.3
DISPENSER .......................................................................................................... 22
3.4
X-AXIS MOVEMENT ................................................................................................ 23
3.5
THE SYRINGE UNIT................................................................................................ 23
3.6
THE TRANSFER FUNCTION ...................................................................................... 25
3.6.1
TRANSFER FUNCTION PARAMETERS ............................................................................. 25
3.6.2
THE DOUBLE DISPENSING NEEDLE .............................................................................. 25
3.6.3
PRIMING OF THE DISPENSING AND WASH CIRCUIT ............................................................ 26
3.6.4
WITHDRAWAL .................................................................................................... 26
3.6.5
DRYING OF THE TIP .............................................................................................. 28
3.6.6
PERFORATION OF THE CUVETTE MEMBRANE .................................................................... 28
3.6.7
LEVEL DETECTION ................................................................................................ 29
3.6.8
MIXING............................................................................................................ 30
3.6.9
USING A PREDILUTED SAMPLE .................................................................................. 31
3.6.10
SIMPLE TRANSFER ................................................................................................ 31
3.7
CALIBRATIONS AND CONTROL PARAMETERS ............................................................ 33
3.7.1
CIRCUIT TRANSFER .............................................................................................. 33
3.7.2
DISPENSERS ...................................................................................................... 34
3.7.3
X-AXIS MOVEMENT ............................................................................................... 35
3.7.4
LEVEL SENSORS .................................................................................................. 36
3.8
FUNCTIONAL TESTS............................................................................................... 37
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3.8.1
TRANSFER TEST................................................................................................... 37
3.8.2
STRIP PERFORATION .............................................................................................. 38
4
OPTICAL UNIT ......................................................................................... 40
4.1
LIGHT SOURCE...................................................................................................... 40
4.2
THE OPTICAL DEVICE ............................................................................................. 41
4.3
THE OPTICAL CHANNEL .......................................................................................... 41
4.4
POSITIONING OF FILTERS ...................................................................................... 42
4.5
OPTICAL CALIBRATION .......................................................................................... 42
4.5.1
TRANSMITTANCE AND ABSORBANCE ............................................................................ 42
4.5.2
ALIGNMENT OF THEOPTICAL FIBRES ............................................................................ 44
4.5.3
CALIBRATION OF THE OPTICAL CHANNELS...................................................................... 46
4.5.4
CALIBRATION OF THE DARK ELECTRONIC OFFSET.............................................................. 46
4.5.5
CALIBRATION OF LIGHT EMISSION .............................................................................. 47
4.5.6
THE CONTROL RAMP .............................................................................................. 48
4.5.7
REPORT OF THE VIRTUAL RAMP .................................................................................. 49
4.5.8
CONTROL WINDOW ............................................................................................... 51
4.6
OPTICAL UNIT TESTING PROCEDURE ....................................................................... 52
4.6.1
SETTING OF THE OPTICAL FILTER OFFSET ...................................................................... 52
4.6.2
OPTICAL CHANNELS ........................................................................................ 52
4.6.3
CHECKING OF THE LAMP’SCONTROL VOLTAGE ................................................................. 52
4.6.4
CALIBRATING THE OFFSET (DARK READING)................................................................... 53
4.6.5
CALIBRATING THE LIGHT......................................................................................... 54
4.6.6
CALIBRATION CURVE ....................................................................................... 55
5
WASHING UNIT ....................................................................................... 58
5.1
HYDRAULIC WASH CIRCUIT .................................................................................... 58
5.2
TANK PROBES ....................................................................................................... 59
5.3
THE WASHER ........................................................................................................ 60
5.4
THE DRYING STATION ............................................................................................ 61
5.5
COLLECTION WELLS .............................................................................................. 62
5.6
WASHING PROCEDURE........................................................................................... 63
5.7
WASHING CIRCUIT TESTING PROCEDURE ................................................................ 64
5.7.1
SUPPLYING OF BUFFERFROM WASHER #1 ...................................................................... 64
5.7.2
SUPPLYING OF BUFFER FROM WASHER #2 ..................................................................... 64
5.7.3
WASHING OF THE PIPINGFOR WASHER #1 .................................................................... 64
5.7.4
WASHING OF THE PIPING FOR WASHER #2.................................................................... 65
5.7.5
ASPIRATION AT DRYING STATION #3 .......................................................................... 65
5.8
CONTROL PARAMETERS.......................................................................................... 65
5.8.1
FILLING LEVEL OF WASHER #1 ................................................................................. 65
5.8.2
FILLING LEVEL OF WASHER #2 ................................................................................. 66
6
THE DRAIN CIRCUIT ................................................................................ 67
6.1
THE MAIN WASTE WELL ......................................................................................... 67
6.1.1
NORMAL OPERATION ............................................................................................. 68
6.1.2
OBSTRUCTED DRAIN ............................................................................................. 69
6.1.3
FULL WASTE TANK ................................................................................................ 69
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6.1.4
6.2
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FAULTY DRAIN PUMP ............................................................................................. 70
WASTE CIRCUIT TESTING PROCEDURE .................................................................... 70
6.2.1
CHECKING OF WASTE LEVEL WARNING SENSOR S3 ........................................................... 70
6.2.2
CHECKING OF ERROR SENSORAND WASTE LEVEL .............................................................. 70
7
TEMPERATURECONTROL .......................................................................... 72
7.1
TEMPERATURE CONTROL OF THE INSTRUMENT ......................................................... 72
7.2
MEASURING CHAMBER ........................................................................................... 73
7.3
HEATER................................................................................................................ 74
7.3.1
ELECTRICAL PROPERTIES OF THE HEATER ...................................................................... 74
7.4
CYCLE TEMPERATURE ............................................................................................ 75
7.5
STAND-BY TEMPERATURE ....................................................................................... 75
7.6
PROCEDURE FOR TESTING THE TEMPERATURE CONTROL SYSTEM .............................. 75
7.6.1
CHECKING OF THE TEMPERATURE SENSOR ..................................................................... 75
7.6.2
CHAMBER TEMPERATURE CONTROL ............................................................................. 75
7.6.3
PROGRAMMING THE STAND-BY TEMPERATURE ................................................................. 76
8
SPEAKER-PRINTER DISPLAY.................................................................... 77
8.1
DISPLAY ............................................................................................................... 77
8.2
THE SPEAKER ....................................................................................................... 77
8.3
THE PRINTER ........................................................................................................ 78
8.3.1
9
PRINTER TESTING ................................................................................................ 78
ELECTRONIC PARTS ................................................................................. 80
9.1
GENERAL MAP ....................................................................................................... 80
9.2
CPU 2010 BOARD AND DRIVER 2010 BOARD ............................................................ 86
9.2.1
POWER SUPPLY ................................................................................................... 86
9.2.2
DESCRIPTION OF THE TEST POINTS AND CPU 2010 BOARD JUMPERS ..................................... 95
9.2.3
DESCRIPTION OF THE TEST POINTS AND THE DRIVER 2010 BOARD JUMPERS ............................ 96
9.2.4
TROUBLESHOOTING .............................................................................................. 97
9.3
LOW POWER ......................................................................................................... 98
9.3.1
DESCRIPTION ..................................................................................................... 98
9.3.2
POWER SUPPLY AND CONNECTIONS ............................................................................ 98
9.3.3
TROUBLESHOOTING ............................................................................................ 103
9.4
POWER SUPPLY ....................................................................................................104
9.5
DESCRIPTION ......................................................................................................105
9.5.1
9.6
TROUBLESHOOTING ............................................................................................ 109
CONNECTORS BOARD ...........................................................................................110
9.6.1
DESCRIPTION ................................................................................................... 110
9.6.2
TROUBLESHOOTING ............................................................................................ 113
10
SERVICE PROCEDURES .......................................................................... 114
10.1
PROGRAMMING CPU 2010 ..................................................................................114
10.1.1
SERVICE APPLICATION ......................................................................................... 114
10.1.2
CONNECTION WITH CHORUS TRIO SUCCESSFUL ............................................................ 115
10.1.3
SAVING METHODS .............................................................................................. 117
10.1.4
CONNECTION WITH CHORUS TRIO UNSUCCESSFUL ......................................................... 118
10.1.5
LOADING OF PARAMETERS AND METHODS.................................................................... 120
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10.2
CHSMIT30
PROGRAMMING THE TC1100 .............................................................................. 123
10.2.1
PREREQUISITES................................................................................................. 123
10.2.2
CONNECTING THE READER TO THE PC ....................................................................... 123
10.2.3
INSTALLATION OF THE CONFIGURATION SOFTWARE ......................................................... 123
10.2.4
LAUNCH THE CONFIGURATION PROGRAM ..................................................................... 124
10.2.5
WIRING DIAGRAM OF THE CONNECTION CABLE .............................................................. 127
10.3
PROGRAMMING THE DLC6065 BARCODE READER ................................................. 128
10.3.1
CONNECT THE READER TO THE INSTRUMENT THROUGH THE RS232 CABLE .............................. 128
10.3.2
DISCONNECT THE RS232 CABLE ............................................................................. 129
10.3.3
ENABLING SERIAL COMMUNICATION .......................................................................... 129
10.4
PROGRAMMING BARCODE READER ZEBEX Z 3080 ................................................ 130
10.4.1
CONNECT THE READER TO THE INSTRUMENT THROUGH THE RS232 CABLE .............................. 130
10.4.2
DISCONNECT THE RS232 CABLE ............................................................................. 130
10.4.3
PROGRAMMING PARAMETERS ......................................................................... 131
SYSTEM SETTING.......................................................................................................... 132
QUICK SETTING ............................................................................................................ 133
10.5
MAINTENANCE ................................................................................................. 134
10.5.1
ROUTINE MAINTENANCE ....................................................................................... 134
10.5.2
PERIODIC MAINTENANCE ....................................................................................... 135
10.5.3
WASH WELL FOR THE DISPENSER NEEDLES .................................................................. 137
10.5.4
DISPENSERS #1 AND #2 ..................................................................................... 138
10.5.5
X-AXIS GUIDE .................................................................................................. 139
10.5.6
WASHERS 21 – 25 – 28 ..................................................................................... 140
10.5.7
OPTICAL UNIT................................................................................................... 141
10.5.8
WASHER WELLS ................................................................................................ 145
10.5.9
UPPER PLATE .................................................................................................... 145
10.5.10
CAROUSEL ...................................................................................................... 146
10.5.11
PERISTALTIC PUMPS ............................................................................................ 146
10.5.12
DIAPHRAGM PUMP .............................................................................................. 147
10.5.13
HYDRAULIC WASTE AND SYRINGE UNIT....................................................................... 147
10.5.14
HYDRAULIC UNIT ............................................................................................... 148
10.5.15
SYRINGE UNIT .................................................................................................. 149
10.5.16
ROTATION ....................................................................................................... 150
10.5.17
TRAY SYNCHRONIZATION DEVICE ............................................................................. 150
10.5.18
LAMP BOX AND FILTER HANDLER
10.5.19
TANK PROBES ................................................................................................... 151
10.5.20
TUBING .......................................................................................................... 151
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1 MOVING THE STRIPS
1.1 THE WORKSTATIONS
The functioning of the system is based on the carrying out of hydraulic, optical
and mechanical operations in certain positions called workstations. The
workstations are mounted on a base located above the tray called the upper
level, shown in the figure below:
1
2
6
28
25
22
7
21
12
fig. 1.1
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Pos
Function
1
fluid transfer station, where liquids are transferred from one well to another. The positioning unit is
able to move from one well to another and to transfer the liquids.
2
1st reading station
6
station where the presence of the strip is checked
7
station for the reading of the strip’s barcode
12
2nd reading station
22
3rd reading station
21
1st well washer for the strip
25
2nd washer
28
3rd washer (drying)
Since the strip has two reaction cuvettes, wash and reading stations are created
so that the operations can be performed on both cuvettes simultaneously.
1.2 THE TRAY
The device in which the strips are inserted. Composed of a circular plate, hinged
on one side with a pulley on the other, and is able to rotate between the two
levels (one upper and one lower), strengthened by four columns. The support
surfaces also border the upper and lower part of the measuring chamber, which
is completed with the special circular plastic crown fastened around the tray.
The tray is locked in place by tightening the locking ring-nut (see fig. 2.2).
1.3 TRAY ROTATION
The rotation direction of the tray is determined by a motor whose shaft is joined
to a pulley which drives the rotor by means of a rotational belt.
The motor is mounted on a tensioning bracket so that the belt can be tightened
to one’s liking. Tensioning is done by turning the adjustment screw so that the
bracket moves closer or farther from the tray’s axis. A temporary fastening screw
locks the tensioning bracket in place so that the belt tightness can be checked.
Locking the fastening screws on the bracket holds the motor in position and
maintains the belt tension.
Tray positioning is checked by:
1) absolute positioning sensor (7) which sets the mechanical zero position
2) relative hole sensor (13) that check the movement of the tray (step check)
3) synchronization device abbreviated TSD (6) that allows the tray to be
locked in each of the work positions.
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1
2
3
5
8
12
4
13
11
7
6
9
No.
Description
1
Locking ring-nut
2
Upper surface
3
Column
4
Lower surface
5
Strip tray
6
Tray synchronization device (TSD)
7
Absolute tray sensor
8
Tray shaft with pulley
9
Drive belt
10
Drive shaft with pulley
Temporary lock nut
11
12
13
10
Tensioning bracket
Hole sensor (relative)
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3
2
4
1
No.
Description
1
Motor
2
Temporary fastening nut
3
Motor unit movement slide
4
Belt tensioning screw
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1
1.
2.
3.
4.
5.
6.
2
13
3
12
4
5
11
9
8
10
7
6
CHSMIT30
locking ring-nut
upper surface
column
lower surface
strip tray
Tray Synchronization
Device (TSD)
7. absolute tray sensor
8. drive shaft with pulley
9. drive belt
10. motor with pulley
11. temporary
fastening
nut
12. tensioning bracket
13. hole sensor
(relative)
1.3.1 ABSOLUTE SENSOR
A small magnet is mounted on the rotational pulley of the drive shaft which is
detected by a Hall sensor located on a small plate fastened by an angular
bracket. The magnet ensures an absolute mechanical zero position and therefore
alignment of the tray.
1.3.2 STEP CHECK
Every step that the tray makes during the cycle needs to be checked to ensure
that it was done correctly.
The check is done using an infra-red reflection sensor that “detects” the passage
between the plate full area (illuminated sensor) and the area with the hole (dark
sensor).
The rotation control software analyses the time intervals of the passages
between the dark zones and the illuminated zones and checks that the rotor is
turning correctly.
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1
1. hole sensor (relative)
2. reference hole
2
1.3.3 SYNCHRONIZATION
Once the rotation is finished, or after the shaking of the plate has ended, the tray
is correctly positioned by a mechanical unit made up of a special device called
the Tray Synchronization Device (TSD) or simply the tray synchronizer, which
inserts a cone-headed cylinder in one of the holes beneath the tray that
correspond to each of the 30 strip insertion positions.
The wedge-shaped pin is quickly moved by a motor on which a worm screw is
mounted. Two sensors check the positioning. The first (limit switch) checks the
starting mechanical position (detail A in the figure). The other is used to check
the alignment of the plate before carrying out the complete insertion of the pin
(detail B in the figure). At the end of the operation the pin completely enters the
hole and locks the tray in the desired position. (detail C in the figure).
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2
1
3
4
A
1. conical pin of the TSD
2. positioning hole
3. relative position sensor
B
4. absolute sensor (at
rest)
C
1.4 STRIP HOUSING
The 30 strips sit in radial slots situated 12° from each other, which were specially
shaped in order to assure easy insertion and to prevent the strip from moving
during the movement of the plate (rotation and mixing). Above all, the seat
allows the strip to maintain the same positioning under the three optical reading
stations.
The strip is inserted in the slot and is kept in position by the spring located at the
end of the slot, which keeps the strip locked in position by holding the last well.
Upon insertion the strip must be pushed to the bottom of the seat so it can be
held by the spring.
The insertion of the strip into the slot is aided by the bevelling of the lower edge
of the plate.
There are two holes at the bottom of every slot that allow the optical ray to pass
through. The diagram below shows the top view of a plate with 28 strips fully
inserted, one strip partially inserted and one position empty.
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1. spring locking sector
2. spring
3. holes for optical reading
1
4. strip slot
2
5. strip
6. strip slot (front view)
7. bevel for strip insertion
4 3
5
7
6
1.5 TRAY LOCK
When the instrument is switched off, or there is a power outage, the manual or
random rotation of the tray needs to be blocked in order to prevent damage to
the dispenser needles and to the workers. This function is carried out by the Tray
Lock Device (TLD). It is composed of an electro-magnet which is released and
mechanically locks the tray when the power is cut off. Vice versa, when the
system is powered up, the mechanical lock is removed and the tray may be
moved by the control system.
2
1. lock pin
2. centering hole
3. solenoid
1
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1.6 CALIBRATIONS AND CONTROL PARAMETERS
CHSMIT30
1.6.1 ALIGNMENT AND CENTERING OF THE PLATE.
Plate alignment is one of the operations for setting the instrument.
The aligning of the plate is designed to position to plate in the exact work point
where the following conditions are checked:
 Plate position no. 1 must be centred with the dispenser slit; in particular,
the direction of the dispensing needles must be centred with the strip
cuvettes.
 Cuvettes no. 5 and 6 of the strips must be perfectly centered with the two
optical channels of the interleavers.
The plate is aligned when the TSD enters the tray cavity without generating any
visible movement of the tray.
The operations to perform in order to align the tray must be done using the
Chorus Manager service program.
The plate alignment is checked with the tray zero offset parameter which
represents the number of steps the tray must carry out to be aligned, starting
from the absolute position which is determined by the absolute sensor located in
the rotation device. The following operations can be performed to check the
calibration:
1. In the Settings / Hardware Parameters / Mechanical Calibrations window set
the Tray zero offset parameter to zero.
2. Use the Commands / Hardware Commands /Motors-Tray command. The
carousel turns clockwise and stops without activating the TSD, but plate
position no. 1 does not correspond to the dispenser slit since the tray stopped
in front of the absolute sensor.
3. Use command Commands / Hardware Commands / Positions -Tray and make
the tray cover the number of steps necessary to become aligned, then insert
the TSD:Insert TSD- Tray Synchronization Device.
4. In the Settings / Hardware Parameters / Mechanical Calibrations window set
the Tray zero offset parameter to the number of steps determined
previously.The ideal value must be < 80.
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2 STRIP RECOGNITION UNIT
2.1 STRIP PRESENCE SENSOR (SPS)
The Strip Presence Sensor (SPS) is composed by a light emitter located on the
upper level and a corresponding receiver located on an electronic board, located
in a special housing on the lower level.
If a strip has been inserted into the tray housing, the handle stops the light
beam and the receiver can detect the presence of the strip. An unlabelled strip
cannot be recognized.
1
A
2
1. light emitter
2. receiver
B
2.2 EXTERNAL BARCODE READER
The CCD ZEBEX reads barcodes automatically as well as on contact. The front
window projects a line of light which must cross the entire code.
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The best conditions for the reading are obtained when the reader handle is kept
parallel to the surface on which the code is found.
2.3 INTERNAL BARCODE READER
The barcode is read at station no. 7 where there is an automatic scanning
barcode reader, which is represented below:
1
2
3
4
1.
2.
3.
4.
5.
barcode reader
reader bracket
barcode reading beam
tray upper surface
strip being read
5
2.4 CALIBRATIONS AND CONTROL PARAMETERS
2.4.1 SPS (STRIP PRESENCE SENSOR)
1. put an unlabelled strip in position no. 1 of the plate
2. reset the carousel (Commands / Hardware controls / Reset-Tray) and move it
to 400 steps using the Positions / Tray / command
3. check that the strip presence sensor is active from the Commands / Hardware
controls menu
4. repeat steps 2 and 3 by incrementing the steps in multiples of 10 steps and
check that the strip presence sensor remains active between 380 and 410
steps.
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2.4.2 EXTERNAL BARCODE READER
2.4.2.1
CHSMIT30
Connecting the reader to the instrument
Connect the cable to the serial port labelled Barcode located in the left rear
portion of the Chorus trio and then tighten the fastening screws on the
connector.
Warning: since the serial port also supplies power to the reader, it is best to connect it when the
instrument is off.
2.4.2.2
Enabling serial communication
To use the reader on the Chorus trio, its serial communication (RS232) must be
enabled by sequentially scanning the three barcodes reported in the table below:
1. The reader goes into configuration mode by scanning the first barcode
(command $+)
2. The reader enables the serial interface by scanning the second barcode
(command CP0)
3. The reader saves and exits the configuration mode by scanning the third
barcode (command $-)
Note: This operation can be done from any software window.
2.4.2.3
Testing
1. With the machine switched off, connect the barcode reader to the serial port
labelled BARCODE, located on the back of the instrument.
2. Switch on the machine and wait for Chorus Manager to connect.
3. Now go to the Commands menu and then to the Hardware Controls menu
4. Scan a code from a valid strip and check in the window that it was read. Click
on External barcode and check the correctness of the reading.
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2.4.3 INTERNAL BARCODE READER
1. Reset the TSD (Reset / Tray Synchronization Device (TSD)) to unlock the
carousel
2. Disable the motors by means of the Disable all button
3. Insert a strip with a valid code and manually move the carousel until the strip
is aligned with the internal barcode reader
4. Click on the Barcodes folder and select Internal, then check that the code of
the strip was read correctly
3 TRANSFER UNIT
3.1 DISPENSING HYDRAULIC CIRCUIT
The part of the hydraulic circuit that supervises the fluid transfer operation is
shown in the following diagram:
closure
cap
250ul
Bput
CS sen.
(1-4)
SV8
SV9
ON
SV3
Aput
ON
disp #1
SV7
disp #2
ON
closure
cap
SV4
level sensor
ON
Pp3
level sensor
SV5
Sput#1
Sput#2
Pasc
Pasc
Plav
pp3:
the peristaltic pump that takes in wash water from the tank and distributes it inside the
instrument.
SV3:
enables the connection of the dispensing needles or the liquid coming from pump pp3
or the syringes.
SV4:
enables the delivery of water for the external washing of the needles
SV5:
enables the external wash flow on disp1 or disp2.
SV7:
enables the flow selected by ev3 on disp1 or disp2.
SV8:
guides the inlet/outlet flow from the 250 ml syringe.
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SV9:
---
CHSMIT30
As can be seen in the figure, each needle has a pair of wells(Plav, Pasc) with the
following characteristics:
-
and internal spout that delivers a jet of water onto the tip of the needle when
it is inserted into the well.
-
The drying well Pasc has a large mesh sponge that retains any drops of wash
water that collect on the outer side of the needle.
Each of these wells has a drain that directs the water to the main waste well.
The syringe is a device that precisely draws up and dispenses the liquids. The
syringe has a capacity of 250 µl. It is connected to the wash water tank, as the
circuit connecting it to the needle is filled with wash water.
Every needle has a capacitive level sensor that is able to detect a minimum of 40
µl and has a sensitivity of 10 µl. The inside and outside of every needle tip is
covered with a ceramic material that reduces carry-over to almost zero.
3.2 THE STRIP, NEEDLES AND THE WELL
The figure below give a cross-sectional view of the physical layout of the strip,
the wash needles and their wash well:
1
6
#2
#1
5
7
8
9
10
4
3
2
11
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
measuring cuvette (#6)
measuring cuvette (#5)
sealed cuvette
cuvette with serum
independent up-down movement
independent up-down movement
radial movement for the two needles
drying well
wash well
dispensing needle
needle wash nozzle
waste duct
12
As shown, the pair of dispensing needles is mounted on a bracket which
simultaneously moves them in a radial manner with respect to the tray (X-axis),
while each can move independently in a vertical direction.
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3.3 DISPENSER
The dispenser is a device that is designed to move the dispensing needle up and
down within the wells of the strips, for drawing up or dispensing liquid.
The dispensing needle is a stainless steel tube with its tip cut at 90°, and which
has been sharpened to help cut through the protective film of the wells. The
coarseness of the end of the needle, both internally and externally, is increased
by a micrometric ceramic deposit that helps reduce carry-over to a minimum.
The upper part of the needle is shaped to stop the tube.
The needle is also used as a level sensor, by connecting it to a piezoelectric
circuit through a special screened cable soldered to the needle.
It is mounted on an insulating block, inserting it in a pass-through hole (see
cross section A-A) and compressing the spring inside the block with the cylinder,
which enlarges the section.
The two prongs of the block’s fork are then inserted in the special holes to
prevent the needle from coming out of position. The thrust of the spring ensures
needle stoppage. The stability of the needle is also ensured by the fork which
stops the tube.
The needle block is mounted on a bracket that is connected to the slider which
moves up and down along the slide. The bracket is integrated with a rack and
therefore the rotation of the motor, with flush fit shaft pinion, produces the
vertical movement of the needle.
A magnetic sensor mounted on an electronic board with magnets mounted on a
connecting rod is used to set the starting vertical position.
A U-shaped aluminium bracket supports all the parts and its base is fixed to the
sliding base plate of the Dispenser Carriage.
sinistra
destra
A
1.
2.
3.
4.
5.
6.
7.
2
1
14
3
13
12
4
5
15
6
7
11
8
16
17
10
9
18
A
DESTRA
FRONTE
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SINISTRA
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
bracket
tube
connecting bracket
needle
level sensor wire
needle cylinder block fork
insulated needle-holding
block
slider
slide
toothed rack
pinion
motor
position sensor
fork
slider for the drum slide
spring
tapering
tip
sezione
A- A
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3.4 X-AXIS MOVEMENT
The moving of the two dispensers along the strip is carried out by an X-axis
carriage, which is a device assembled on the upper surface of the instrument’s
frame. The dispensers are fastened to the base of the bracket on the sliding
carriage where the holes for the fastening screws are located.
The carriage runs along the slide since it is fastened to the slider (which cannot
be seen in the diagram). The carriage is attached to the toothed belt by a hook
that comes out of the belt.
The toothed belt is driven by a motor through the pulley. The other end of the
belt is kept taut by a base plate on which an idle pulley is fastened.
The motor is fastened to the upper surface by an angular bracket. The position of
this angular bracket can be adjusted along the special slots, so that the belt
tension can be modified.
A magnetic sensor mounted on an electronic board and the relative magnets
mounted on sliding carriage is used to set the start position of the carriage.
To allow the dispensing needles to reach the wash well, a special passage area is
opened on the upper surface in the area for accessing the strip area.
3
5
4
6
7
8
9
2
10
1
11
1.
2.
3.
4.
12 5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
drive hook
frame’s upper surface
motor
angular bracket
adjustment slots
motor pulley
position sensor
sliding carriage
threaded holes
wash access area
slide
access to the strip wells
toothed belt
idle pulley
base plate pulley
13
15
14
3.5 THE SYRINGE UNIT
The function of the syringe unit is to move the syringe for the withdrawal and
dispensing of liquids.
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The unit is made up of a stepper motor connected by a joint to a worm screw.
Based on the motor’s direction of rotation, this unit acts on the sliding block and,
therefore, the slider mounted on its lower part, to provide movement in one
direction or the other.
For positioning, the magnetic sensor mounted on the electronic board and the
corresponding magnet mounted on the slider is used.
Two Teflon pads allow the slider to move fluidly over the guide slot of the base
plate.
The thrust plate and the end bracket keep the worm screw in line through the
use of internal bearings.
The upper part of the two syringes are coupled to the Plexiglas syringe unit and
the middle portion of the syringes is coupled to the clamping bracket. The
pistons are mounted on the fixing bracket located on the slider.
Based on the amount of liquid to be processed, the solenoid valve (14) mounted
on the top of the syringe unit opens and closes the syringe lines, thus allowing
the instrument to withdraw or dispense liquids.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
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motor
joint
thrust plate
worm screw
sliding block
slider
end bracket
pads
guide slot
syringe unit
syringes
clamping bracket
fixing bracket
solenoid valves
position sensor
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3.6 THE TRANSFER FUNCTION
CHSMIT30
3.6.1 TRANSFER FUNCTION PARAMETERS
To proceed in describing the transfer function, the parameters that characterize
it
should
first
be
described.
The table below lists the parameters that will be described in detail in the
successive paragraphs. Firstly, every transfer must be identified with an
identification number (ID) in order to be recognized for the operating procedure
of
a
cycle.
ID
Transfer Identification Number. A value of 0 means that no transfer will be
performed. Refer to this value in the archive Methods / Transfer settings and in
Methods / Mode settings.
Family
This field is designed to optimize the cycle. During the pre-cycle phase, transfers of
the same family are done in sequence. This greatly reduces the number of washing
operations.
Needle
Needle to use for the transfer. The possible values are 1 (standard) or 2
(conjugated).
Starting cuvette
Starting cuvette of the strip, from which the liquid is withdrawn. The possible values
range from 0 to 7.
Syringe
Syringe type. The value of 0 is for that of 250 µl.
Start delay
Delay, in seconds, before starting the transfer. Some methods require a delay from
10 to 15 seconds before starting.
Cuvette #1
Number of the first destination cuvette. The possible values range from 0 to 7.
Quantity #1
Amount of liquid to transfer into the first well. Possible values range from 0 to 250 µl.
Cuvette #2
Number of the second destination cuvette. The possible values range from 0 to 7.
Quantity #2
Amount of liquid to transfer into the second well (if required). Possible values range
from 0 to 250 µl.
Wash mode
This field indicates the mode for washing the needle after the Transfer. The values
range from 0 to 6.
Shaking number
The number shakes to perform in the last cuvette. The maximum number is 6 (from
0 to 5).
Shaking quantity
Amount of mixed liquid. The maximum value is 100 µl.
3.6.2 THE DOUBLE DISPENSING NEEDLE
To prevent the conjugate from coming into contact with the substrate, even
minimally, a dedicated withdrawal needle must be used. This needle must have
the ascent/descent movement and all the other controls, independent from the
other. Operation is completely interchangeable from a hydraulic point of view.
Solenoid valve Ev7 selects which of the two needles must operate.
•
Disp no. 1 is dedicated to the transfer of serum, diluent and substrate
•
Disp no. 2 is dedicated to the conjugate.
In a transfer, the Needle parameter establishes which needle must be used.
•
Needle = 1 sets the use of dispenser no. 1
•
Needle = 2 sets the use of dispenser no. 2
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3.6.3 PRIMING OF THE DISPENSING AND WASH CIRCUIT
CHSMIT30
To ensure correct dispensing, all the hydraulic circuits involved in dispensing
must be properly filled.
In particular:
-
the section of the circuit that goes from the cleaning solution tank to the
syringe
-
the syringe
-
the section connecting ev3 to ev7
-
the tubing that connects the two outlets of ev7 with the dispensing needles
-
the needles (2/3 of their capacity, thus leaving the entire tip dry).
This operation is managed using an automatic procedure by the instrument
which uses the syringes to fill all the tubes and checks that it was done using the
main waste well warning sensor.
The tubing in which wash water flows must be completely filled so that the
system functions properly for all the tests.
To do this, allow water to flow through the tubes until it exits into the main
waste and can be detected. This procedure is also performed automatically when
the instrument priming is started.
3.6.4 WITHDRAWAL
The removal of liquid from a well, done at the cuvette bottom level, which
guarantees that the entire contents of the well can be drawn up.
Starting cuvette is the starting cuvette (or well) number from which the liquid
must be drawn up.
The dispensing function is organized so that, after the liquid has been withdrawn
from the starting cuvette, the amount equal to the Quantity #1 parameter can
be dispensed into the cuvette defined to be Cuvette #1 and the amount equal to
the Quantity #2 parameter can be dispensed into the cuvette defined to be
Cuvette #2. The amount withdrawn from the Starting cuvette is therefore the
total of the Quantity #1 and Quantity #2 amounts.
The amount withdrawn is actually increased by a preset amount, in order to
prevent the final emptying from coinciding with a bubble.
-
For quantities up to 20 μl and if the large syringe is used, a total of 25 μl is
taken.
-
For quantities from 21 μl to 100 μl, an extra 5 μl is taken.
-
For quantities above 100 μl, an extra 20 μl is taken.
When the amount of liquid is destined for mixing, no extra amount is taken.
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3.6.4.1
CHSMIT30
Needle washing
The needle must be washed after every transfer.
The washing is done in two stages:
1. washing of the internal part
which involves (ex. washing of disp1):
-
positioning of the pair of needles over the respective wash well with an x-axis
movement (step 1)
-
descending into the well to a predetermined depth so that the external nozzle
coincides with the start of the needle’s tapering
-
activation of ev3 so that water flows towards ev7
-
activation of pump pp3, putting wash water into circulation
-
ev7 is not controlled and liquid overflows into the collection well, which drains
by gravity into the main waste well (step 2).
2. washing of the external part
which involves (ex. washing of disp1):
-
activation of ev4 so that water flows towards ev5
-
activation of pump pp3, putting wash water into circulation.
-
ev5 is not controlled and liquid comes out of the wash nozzle and washes the
outside of the needle and then drops into the collection well, which is then
drained by gravity into the main waste well (step 3).
-
simultaneous activation of the needle’s slow ascent from the well until the
nozzle reaches the tip of the needle and externally wash the entire needle
(step 4)
-
shutting off of pump pp3 and ev4 and return to the resting position (step 5).
1. ready for washing
2. internal washing
3. first
external
washing stage
4. last
external
washing stage
5. final resting position
1
2
3
4
5
The duration of the needle washing is defined in the Wash mode parameter,
which is set whenever a certain transfer procedure is defined.
It can have a value from 0 to 5, according to the following table.
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Washing
description
0
No washing
1
10-15 ml water
2
3
4
5
The final washing of the needle is skipped if the next transfer belongs to the
same family as that being processed. This functional characteristic can save
considerable amounts of wash water.
3.6.5 DRYING OF THE TIP
After washing, the needle remains immersed in the Plav, where the water drops
begin to drip off the inside and outside of the needle. These drops must be
completely removed at the start of a transfer procedure. To do this, the needle
needs to be inserted into the drying well, Pasc.
The steps are the following:
1. Ascent from Plav
2. Shifting to Pasc
3. Descent into Pasc and drying of the needle
4. Ascent from Pasc.
Drying is only done if the tip of the needle was washed.
3.6.6 PERFORATION OF THE CUVETTE MEMBRANE
The test strip requires that some wells, containing liquids or lyophilized product,
be sealed with a thin plastic membrane. The withdrawal and dispensing of liquids
in the sealed cells requires that the membrane be pre-bored in a position
different from that of the successive suction hole.
The pre-boring creates a hole in the membrane where air can safely enter.
This is necessary since the sides of the hole made in the membrane for
withdrawing can seal around the needle itself, thus reducing or preventing air
from entering.
The lack of incoming air into the well during the suction phase causes a lesser
amount of liquid to be removed with respect to that programmed, thus leading to
measurement errors.
Pre-boring is done automatically by the procedure, before proceeding with the
suction hole. Nevertheless, this is avoided if the well is already perforated.
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3
2
CHSMIT30
4
1
1.
2.
3.
4.
5.
cuvette bottom level
membrane
pre-boring
suction hole
sharp needle tip
5
The dispensing needles are made with oblique tips with sharp edges. The cutting
of the membrane may be imperfect or even poorly cut due to the fact that the tip
becomes dull over time (life expectancy of more than 3000 cuts) or if the membrane is too
tough due to an imperfect heat sealing.
A check of the hole and the tip’s cutting ability must therefore be included for
every perforation operation.
Use the following procedure:
1. Descent of the needle at the preset speed, starting from the home vertical
level, down to the cuvette bottom level and withdrawal of liquid (for preboring, the level is preset and withdrawal is not performed)
2. Return to the home vertical level
If the return to the home vertical level is correct, the difference between the
theoretical number of steps between cuvette bottom level and home vertical
level and that actually performed is calculated.
If the difference is:
 within ± 1 step, the transfer continues,
 within the ± acceptance range: a membrane warning message is sent and the
transfer continues,
 outside the ± acceptance range: a membrane error is sent and the sample is
not processed.
Note that with this condition the return to the home vertical position cannot be
carried out.
In this case, try vertical repositioning two times. If only the first reset is wrong,
the sample is skipped and the run continues. If the second reset is also wrong,
the run is stopped.
3.6.7 LEVEL DETECTION
The level detector, found on each of the needles, is designed to control the
amount of liquids dispensed into the cells of the strip by checking the dispensed
liquid level.
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The sensitivity of the sensor is measured as: minimum detectable amount of
liquid, ≥ 40 μl and min 10 μl discrimination, which indicates the minimum level
difference that can be detected in cuvettes containing at least 40 μl.
One of the fundamental characteristics is that it can also be used for liquids
without ions (ex. distilled H2O).
After dispensing the set amount of liquid, the needle returns to the home
position and then descends again and stops when in contact with the liquid. The
number of steps between the bottom of the cuvette and the detected level is
calculated.
The sensor is used in the following cases:
 detection of the amount of serum present in the well before withdrawal
(verify)
 detection of the amount of diluted serum distributed in the measuring well
 detection of the amount of substrate distributed in the measuring well
 detection of the amount of conjugate distributed in the measuring well
The limiting factor is that it cannot be used for membrane covered cuvettes, as
this would cause measurement errors.
The detection procedure is simple and involves two situations:
1. checking of the level in a cuvette, before the withdrawal of liquid
-
the needle, starting from the home vertical level, descends into the well with
the level sensor on
-
when it stops or has detected the liquid or is at its end stroke, the liquid level
is calculated by the difference in steps.
-
if the amount is insufficient with respect to that expected, an error is reported
and the strip is not processed.
2. checking of the level of liquid that has just been dispensed
-
After the liquid has been dispensed, the needle returns to the home vertical
position
-
it then descends into the well with the sensor on
-
when it stops or has detected the liquid or is at its end stroke, the liquid level
is calculated by the difference in steps.
-
if the amount is insufficient with respect to that expected, an error is reported
and the strip is not processed.
3.6.8 MIXING
After having distributed a liquid in a cuvette which already had other liquid (ex:
serum in the diluent cuvette), the solution needs to be mixed in order to make it
homogeneous.
The shaking of the plate is only sufficient for keeping homogeneous solutions in
suspension.
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Mixing is controlled by two transfer function parameters:
CHSMIT30
•
Shaking number: number of withdrawals/dispensings to perform NumAg
•
Shaking quantity: the amount that is withdrawn and dispensed to perform
the mixing
The initial situation is the following:
1. The needle gets to the bottom cuvette level, where it dispensed the liquid into
the cuvette and the dosing syringe is completely closed.
Warning: the mixing mode requires that the liquid to be mixed be taken without any extra
amount, in order to avoid bubbles from forming.
2. Now, with the needle still at the cuvette bottom level, the Shaking quantity is
drawn in for the first time and then expelled. This is done the number of
times indicated by the Shaking number.
3. When finished, the needle exits the cuvette and is washed.
3.6.9 USING A PREDILUTED SAMPLE
Each test code has a very precise pre-cycle and cycle procedure.
For pediatric samples, the user can only insert the strip into diluted serum due to
the availability of serum. The strip code is the same for the preselected test,
independent of whether the serum has already been diluted or not.
To remedy this problem, a certain type of pediatric sample can be classified
when the samples are introduced. This allows the instrument, starting from the
test code, to perform another transfer procedure, which only differs from the
standard procedure by modifying the sample preparation.
3.6.10
SIMPLE TRANSFER
We will now analyze a simple transfer function using the procedures partially
described beforehand.
There are two types of transfer:
3.6.10.1
Transfer for dilution of the serum
This is the transfer that brings the serum from well no. 1 to well no. 3 and mixes it.
The transfer stages are:
1. Drying of the needle tip
2. withdrawal of Quantity #1 from cuvette 1 (Starting cuvette) without extra amount
3. shifting to cuvette 3 (Cuvette #2)
4. pre-boring and boring of the hole with membrane check
5. dispensing of Quantity #1 mixing
6. shifting to the home position
7. washing of the needle
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#1
#2
1. washing of Disp #1
#1
#2
#2
1. drying
Disp #1
2. disp #1 carries
vertical reset
#1
2
1
#2
#1
#2
#1
2
1
#2
#1
#2
#1
1
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2
out
a
1. disp #1 moves to the
starting
cuvette
and
withdraws Quantity #1
2. disp #1 carries out a
vertical reset
#1
#2
1. disp #1 moves and makes
a hole in the membrane of
Cuvette #1
2. disp #1 carries out a
vertical reset
#1
#2
1. disp
#1
dispenses
Quantity #1
2. disp #1 carries out a
vertical reset
2
1
of
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#2
#1
#2
#1
1
3.6.10.2
2
13. disp #1 is washed and
dried
14. disp #1 returns to the
home position
The transfer of conjugate
The stages involved in this type of transfer are:
1. Drying of the needle tip
2. Withdrawal of Quantity #1 from Cuvette 3 (Starting cuvette) with extra amount.
3. Shifting to well 6 (Cuvette #1)
4. Dispensing of Quantity #1
5. Shifting to the home position
6. expulsion of the extra amount and washing of the needle.
3.6.10.3
Double transfer
The transfer stages for this case are:
1. Drying of the needle tip
2. Withdrawal of Quantity #1+ Quantity #2 from Cuvette #3 (Starting cuvette) with extra amount
3. Shifting to well 5 (Cuvette #1)
4. Dispensing of Quantity #1
5. Shifting to well 6 (Cuvette #2)
6. Dispensing of Quantity #2
7. Shifting to the home position
8. expulsion of the extra amount and washing of the needle
3.7 CALIBRATIONS AND CONTROL PARAMETERS
3.7.1 CIRCUIT TRANSFER
3.7.1.1
Transfer circuit testing procedure
1. Connect a water tank with saline solution and drain tubes to the waste tank.
2. Activate solenoid valve SV4 (Solenoid valves / SV4) and pump PP3 (Pumps /
Pp3)
3. Check that the flow of water comes out of UGEL 1.
4. Activate solenoid valve SV5 (Solenoid valves / SV5)
5. Check that the flow of water comes out of UGEL 2.
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Warning: check that the nozzles that supply wash water are correctly positioned so that the water hits
the needle.
NOTE: since the error check was activated, the warning alarm must activate once the warning level
has been reached and pump PP8 must switch on to empty the waste well.
3.7.1.2
Distribution needle wash test.
Warning: to avoid damaging the needles, dismount them from the wash unit and place the small tubes
inside the wash wells
1. Deactivate the pump and solenoid valves using the Commands menu.
2. Connect the tank filled with clean water
3. Activate solenoid valve ev3 (Solenoid valves / SV3) and pump PP3 (Pumps /
Pp3) and check that water flows from disp1
4. Activate solenoid valve ev7 (Solenoid valves / SV3) and and check that water
flows from disp2
3.7.2 DISPENSERS
3.7.2.1
Cuvettes #5 and #6 bottom level (needle #1)
Number of steps needed to position dispensing needle #1 from the home
position to the bottom of wells 5, 6.
1. Set the parameter (Settings / Parameters / Mechanical calibrations /
Dispenser #1 bottom level), calculating the number of steps needed to touch
the bottom of the well and then subtracting 1.
This parameter can be automatically calculated through the command:
Settings / Automatic adjustments / Cuvette bottom level automatic
adjustment
Through this command, the parameter is calculated and automatically written in the Flash
3.7.2.2
Cuvettes #5 and #6 bottom level (needle #2)
Number of steps needed to position dispensing needle #2 from the home
position to the bottom of wells 5, 6.
1. Set the parameter (Settings / Parameters / Mechanical calibrations /
Dispenser #1 bottom level), calculating the number of steps needed to touch
the bottom of the well and then subtracting 1.
This parameter can be automatically calculated through the command:
Settings / Automatic calibration
Through this command, the parameter is calculated and automatically written
in the Flash
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3.7.3 X-AXIS MOVEMENT
3.7.3.1
CHSMIT30
Setting of the X-axis offset
The start position of the dispensers is controlled by the parameter:Settings /
Parameters / Mechanical calibration / Carriage offset found in the
parameters table.
This parameter is set to 0 in the default file that is initially loaded onto the instrument.
If this parameter is set to 0 and the following command is given:Commands /
Hardware controls / Carriage, the group of dispensers moves towards the end
stroke sensor and stops.
In this condition, the absolute sensor is to be positioned but to perfectly centre
the dispensers over the respective wash wells, the carriage must return within a
certain number of steps (adjustment parameter).
The number of steps is determined by trial:
1. through the Commands / Hardware controls / Motors / Carriage
command and setting Position and Speed the dispensers move to the
correct position
2. The parameter must therefore be reported in the parameters table.
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3. When finished the reset command must be sent and the position of the
dispensers must be checked to ensure that it is correct.
Warning: the two dispensers must be positioned exactly in the centre of the respective wash wells.
3.7.3.2
Serum cuvette position
Represents the number of steps needed to position dispensing needle #1 from
the home position to the centre of wells no. 1 on the strip (serum), when the
strip is inserted in the tray.
The procedure to calculate this parameter is the following:
1. Send the tray reset command through the Tray home position key
2. The number of steps is determined by trial through the Commands /
Hardware controls / Motors / Carriage command
3. The correct position is checked by manually pushing the dispenser down
4. The value calculated in Setting / Hardware parameters / Mechanical
calibration / Sample well position is then reported
3.7.4 LEVEL SENSORS
1. In a strip insert 50µl of liquid in well 5 and 100µl in well 6
2. Start the Prove in / Level sensor prove in command
The Chorus trio resets the tray and moves the x-axis by moving dispenser no. 1
into well no. 5 and successively into no. 6, and it then reads the liquid level.
The same is then done with dispenser no. 2
The number of times that the operation must be done for each dispenser can be
set (from 1 to 255).
Below is an example of a report:
Disp1
Pz 5: Hi: 101 Pz 6: Hi: 96
Pz 5: Hi: 101 Pz 6: Hi: 96
Pz 5: Hi: 101 Pz 6: Hi: 96
……………
Disp2
Pz 5: Hi: 100 Pz 6: Hi: 95
Pz 5: Hi: 99
Pz 6: Hi: 95
Pz 5: Hi: 100 Pz 6: Hi: 95
……………..
The repeatability of every detection must be Vm (mean value) ± 1
The difference between the reading at 50µl and that at 100 µl must be 5 ±1
The printout is composed of the test report.
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3.8 FUNCTIONAL TESTS
3.8.1 TRANSFER TEST
A personalized transfer can be launched through the Commands /
Hardware controls / Macro / Single transfer command in order to check all
the devices involved in the transfer function.
Dispenser
(Disp#1=norm,
Disp#2=conj):
Needle to use for the transfer. The possible values are 1 (standard) or 2
(conjugated).
Source well (0..7):
Start cuvette from where the liquid is withdrawn. The possible values range
from 0 to 7.
Target well (0..7):
Number of the first cuvette in which the liquid will be transferred. The possible
values range from 0 to 7.
Quantity:
Amount of liquid transferred into the first cuvette.
Lyophile:
Indicates the presence of lyophile.
Target well #2 (0..7):
Number of the second cuvette in which the liquid will be transferred. The
possible values range from 0 to 7.
Quantity #2 (µl):
Amount of liquid transferred into the second cuvette.
Cleaning number:
Number of needle washings.
Shakes (0..5)
Number of times the liquid in the cuvette is shaken.
Shake quantity (µl):
Amount of liquid used during shaking. The maximum is 100 µl.
The procedure to perform the test is the following:
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1. Insert a strip with one of the wells filled with liquid.
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2. From the window select all the parameters needed to perform the desired
transfer
3. Start the transfer
If the transfer was successful the instrument will print a report
If, during the test, the instrument deviates more than the number of microlitres
set in the parameters table (Settings / Parameters / Level sensor tolerance), a
second test is performed, after which the instrument prints the report
Below is an example of a report:
NEEDLE: 1 WELL: 2 CHECK STEPS: 119 REAL STEPS: 119
NEEDLE: 1 WELL: 2 CHECK STEPS: 119 REAL STEPS: 111
Sample# 0 step # 0 tr# 300 HI: 107 TH: 106 diff: 1
Sample# 0 step # 0 tr# 300 HI: 108 TH: 106 diff: 2
3.8.2 STRIP PERFORATION
Procedure for testing the capacity to make hole in the strips
1. Insert the desired number of strips, with intact membranes, starting from
position no. 1 of the plate.
2. From the main menu launch Prove-in / Drilling prove-in
3. Select the number of strips to be perforated
The instrument perforates the membranes of all the wells (2, 3, 4 and 7) of strip
no. 1 twice with dispenser no. 1, and then continues with strip no. 2 using
dispenser no. 2.
A report similar to that below is issued while the holes are being made
SAMPLE NR 1
NEEDLE1 Well: 2
check step 119 real step 119
NEEDLE1 Well: 2
check step 119real step 111
NEEDLE1 Well: 3
check step 119real step 119
NEEDLE1 Well: 3
check step 119real step 111
NEEDLE1 Well: 4
check step 119real step 111
NEEDLE1 Well: 4
check step 119real step 119
NEEDLE1 Well: 7
check step 119 real step 119
NEEDLE1 Well: 7
check step 119 real step 119
SAMPLE NR 2
NEEDLE2 Well: 2
check step 119real step 118
NEEDLE2 Well: 2
check step 119real step 112
NEEDLE2 Well: 3
check step 119real step 111
NEEDLE2 Well: 3
check step 119real step 111
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NEEDLE1 Well: 4
check step 119real step 111
NEEDLE1 Well: 4
check step 119real step 111
NEEDLE2 Well: 7
check step 119real step 119
NEEDLE2 Well: 7
check step 119real step 119
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a difference of 15-20 steps between the check and real steps is allowed
Each well has 2 rows of data, since 2 holes are made.
It is recommended that the test be performed by heating the strips for a few
minutes, so that the membrane stretches, like during the cycle.
The printout is composed of the test report.
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4 OPTICAL UNIT
4.1 LIGHT SOURCE
The source is structurally composed of a low cost halogen lamp with parabolic
dichroic filter able to focus the light of all the microfibres that make up the 6
lines of the optical fiber onto the head of the collection cylinder.
The pairs of lines then go to the optical interleavers.
The focused light passes through a monochromatic filter, which is selected based
on the type of test to perform. A heat filter is inserted in order to prevent the
filter and the optical fiber from overheating.
1. optical fibres
2. cylinder
3. monochromatic
filter
4. heat filter
5. lamp
6. power control
7. electronic control
1
2
3
4
5
6
7
The electronic circuit for regulating the control voltage is what makes the lighting
device particular. It is interesting to analyze the control circuit starting from the
power circuit.
The premises are the following:
A)
the light that each line can transmit is different from the other, even
by 30%, depending on the light gradient that covers the head of the
cylinder and the lack of radial symmetry of the line terminals collected in
the cylinder.
B)
If, in the three measuring stations, the same amount of light does
not traverse each cuvette, homogeneity of the measuring points cannot be
reached.
In order for all the lines in an optical fiber to be homogeneous from a light
emission point of view, a calibration procedure, managed by the central
microprocessor, is required that guides the lamp’s electronic control, which in
turn is able to generate approximately 800 voltage values within the lamp’s
operating range of 5V DC to 12 V DC.
Therefore a voltage value is determined for each optical line in order to generate
light beams of the same intensity.
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4.2 THE OPTICAL DEVICE
As shown in the figure, the optical device used to take the measurements, in
each of the three positions, is made up of a light transmitter mounted on the
upper plate and a light receiver mounted on the lower plate
These two parts make up an optical path that is intercepted by the strip, which
passes through it, in order to perform an optical measurement.
The upper part supports two of the six lines of the optical fiber. Each line
terminates with a ferrule, which blocks the fiber capillaries that make up the line.
The light emitted from each line is sent through an optical channel to the first
focusing lens (this is true for each of the two channels on the optical device).
5
8
7
1
2
4
6
3
10
1. optical fiber
2. optical fiber terminus
ferrule
3. optical channel
4. lenses
5. optical fiber support
6. upper surface
7. strip tray
8. strip
9. focalization point
10. lenses
11. optical channel
12. optical receiver
13. receiver card
14. lower surface
9
13
12
11
14
The light ray focuses a 1.5 mm spot about 1 mm from the bottom of the cuvette.
After it has passed through the strip, the light beam is focalized by a pair of dual
lenses on the optical receiver positioned on the electronic receiving board. The
diameter is 3 mm and ensures a good signal/disturbance ratio.
The translated voltage signal is sent from the conditioning electronics to the A/D
converter.
4.3 THE OPTICAL CHANNEL
The optical channel is a unit that has the following parts:
•
an optical fiber line
•
two focusing lenses found on the interleaver’s optical path
•
the sensor and its pre-amplification circuit
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When one of these elements is modified, the characteristics of the optical
channel are modified.
An interleaver thus combines an odd optical channel (the most external,
corresponding to well no. 5) and an even optical channel (the most internal,
corresponding to well no. 6).
4.4 POSITIONING OF FILTERS
The device for positioning the filters moves the filter block forward and backward
in order to position one of the two filters or the empty position in front of the
fiber optic cylinder.
The filter block is mounted on the plate which, in turn, is fastened onto a bracket
(3) that connects it to the slider, which is moved up and down along the slide
(5). The bracket has a toothed rack and therefore the rotation of the motor (8),
with flush fit shaft pinion (7), allows the filter block to move.
A magnetic sensor mounted on an electronic board with magnets mounted on a
connecting rod is used to set the vertical position.
A U-shaped aluminium bracket supports all the parts and is fixed to the support
of the fiber optic cylinder.
1
left
2
right
3
10
11
12
9
8
13
4
7
6
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
bracket
filter block
connecting bracket
slider
slide
toothed rack
pinion
motor
position sensor
plate
filter #1
filter #2
no filter
5
RIGHT
FRONT
LEFT
4.5 OPTICAL CALIBRATION
4.5.1 TRANSMITTANCE AND ABSORBANCE
The transmittance of the solution being measured is the measurement of the
amount of incident light on the receiver.
The higher the value of the received signal, with regard to emitted light, means
more light has passed through the test solution and therefore less turbidity.
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Transmittance is thus the primary measurement that is made in the optical
channel.
In the Chorus trio, the amount of light received (transmittance) is initially
detected as electrical voltage and then converted with a 12-bit A/D into a
number that can range from 0-1023.
0 corresponds to the absolute lack of transmitted light in the optical channel
(dark) and 1023 is the maximum value of receivable light (channel completely
free).
1000
1800
800
1500
1200
600
900
400
600
200
300
00,3
00,4
0,05
0,06
1/16
1/64
1/128
1/512
1/256
0,02
1/32
0,01
0
Transmittance is however a relative measurement that does not take into
account the characteristics of the the optical channel. Therefore, in the presence
of more than one optical channel, a different parameter must be used to have
comparable measurements: absorbance. Absorbance is a measurement of the
ability to absorb (and therefore not allow to pass through) light emitted by the
emitter. The higher the absorbance the lower the turbidity of the solution.
The absorbance (Abs), for an optical channel, in its theoretical formulation, is
given by the formula:
Abs = log (TH20 / Tsoln),
where TH20 is the transmittance value of water,
Tsoln is the transmittance value of the test solution.
This formulation does not consider the problem of using electronic amplifiers and
analog/digital converters. In fact, the transmittance voltage reading of a
transmittance signal is such that the darkness value (theoretical transmittance =
0) is a residual voltage value (Toff.set), which is subtracted from every
transmittance reading.
The formula to be used therefore becomes:
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( TH20 – Toff-dark )
Abs = log _______________
(Tsoln – Toff-dark )
the measurement includes two parameters
TH20
Toff-dark
transmittance in water
voltage off-set value for the dark
These two parameters are determined:
•
for each optical channel
•
for each filter
and make up the system’s optical calibration phase.
Since the optical calibration must be done in a completely automatic manner,
thus without operator intervention, the measurement of transmittance in water
was made the same as that in air. A modest absolute error is therefore added for
the absorbance differences with regard to the instrument, but which does not
influence the final result.
It should be noted that the assessment of the optical values is done in digital
terms, on a numeric scale that theoretically ranges from 0 to 1023.
This is because the system uses a 10 bit analog/digital converter.
4.5.2 ALIGNMENT OF THEOPTICAL FIBRES
As mentioned previously, the non homogeneity of the light emitted by the
individual optical fibres is an unacceptable work condition since the system
operates by calculating the differences between optical readings made on
individual channels.
Given that an optical reading depends primarily on the light emitted by the light
source and then by the upstream amplification device, six optical fibers must
emit the same amount of light, with a precision on the order of ± 5 ‰.
4.5.2.1
First concept
Given that the amount of incident light on the surface of the group of optical
fibers is distributed according to a gradient that diminishes when moving away
from the centre, and given that the layout of the microfibers inside the collection
cylinder is random and can therefore be arranged more or less towards the
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centre (see example 3), the six optical fibers composed of the microfibers
themselves can have a different luminosity, not because they are physically
different, but because they collect more or less light.
1
1.
2.
3.
4.
2
optical fiber collection cylinder
microfibers
examples of a microfiber unit that makes up an optical fiber
gradient of the incident light field on the surface of the cylinder
3
4
gradiente del campo luminoso
To obtain the same optical response from displaced optical fibers, the incident
light must be different depending on the optical fiber taken into consideration.
This means that the lamp is powered with a different voltage depending on the
optical fiber to be controlled.
4.5.2.2
Second concept
In the Chorus trio system, the time that the tray stays in each of the 30 positions
is never less than 18 seconds, and of this time, no less than 6 seconds is
dedicated to mixing.
Given that, during a step, all six optical channels may need to be read, the time
available for each channel is slightly higher.
VI° fibra
18 sec
vibration time
11 sec
V° fibra
IV° fibra
III° fibra
II° fibra
0 sec
I° fibra
Since the adjustment time for lamp luminosity (from 5V to 12 volt) is less than 2
seconds, emission stability can be obtained for enough time to perform a reading
of an optical channel after this interval.
In each of the time intervals in which the tray waits for the step, it’s therefore
possible to:
-
modify the lamp’s control voltage to the required value, for example for
the 1st optical fiber
-
perform the reading on the relative optical channel.
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120
100
80
60
40
20
0
0
4.5.2.3
1
2
3
4
5
6
7
8
Third concept
The previous concepts indicate the need to have a power supply for the lamp
that can scan the range from 5 to 12 Volts in steps not less than 10 mVolts. This
is to ensure that the six optical fibers are adjusted to within ± 5‰ of the
reference value.
4.5.3 CALIBRATION OF THE OPTICAL CHANNELS
Calibration of the optical channels involves two phases to be performed in order:
1. The determination of the dark offset voltage value
2. The determination of the control voltage of the lamp in order to obtain the
transmittance value in air, set as the reference value.
This is done for each filter used by the instrument.
4.5.4 CALIBRATION OF THE DARK ELECTRONIC OFFSET
This is the first calibration to be performed in order to continue with the “reset”
channel, meaning that it is able to detect valid digital values above 0. By
darkening the optical receiver, the indicated value could be:
digital value > 0
value = 0
In the second case, the digital indication at 0 does not mean that the output from the optical channel’s amplifier is
exactly at 0 analogical volts, but could have a negative value due to the amplifier’s inherent offset. If we were to
accept this value, it would mean losing the evaluation of optical values which, in analogical terms, come from the
negative value of the offset up to zero. A digital value for the dark should therefore be set that gives an indication
> 0, for example 20 digit.
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The calibration of the dark offset therefore requires (see diagram) that a voltage
ramp be generated that progresses from -1 Volt to +1 Volt until a value not
greater than 20 digit coming out of the A/D converter is obtained.
optical
receiver
A/D converter
The voltage generator is obtained with the control of a digital potentiometer.
The potentiometer setting value for each optical channel is stored in the
instrument’s Flash-ROM and is loaded at start-up or recalculated after every
optical calibration.
The calibration must be done with the instrument closed, or properly darkened,
so that the interleaver sensors do not receive external light.
4.5.5 CALIBRATION OF LIGHT EMISSION
The transmittance in air measurement coincides with the voltage measurement
for piloting the lamp for each optical fiber.
When the system, for a certain optical filter, measures water, it must read the
maximum transmittance value possible. This value must correspond to 1023 digit
for the system.
Like for the offset, here too it’s best not to reach the limit, otherwise higher
analogical values won’t be recognized, since all readings would be 1023
(saturation).
The light intensity that allows a digital value of 1000 to be read is therefore set
as the maximum reference intensity for each optical fiber and for each of the
available optical filters.
fixed gain
amplifier
light
optical
receiver
power
supply
digital
ramp
generator
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A/D converter
voltage
ramp
generator
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Having then set the gain of the amplification and conditioning circuits of the six
optical channels, the only way to obtain a light reception equal to 1000 is to act
on the lamp’s control voltage by aligning the intensities of the light transmitted
by the six optical fibers (as described previously).
The calibration system of each channel is done through an automatic procedure.
The control voltage of each channel is scanned from 5 to 12 Volts in 10 mV steps
using the “bisection” method, until the value that gives a reading of 1000 is
determined.
The control of the power occurs digitally and the digital value that corresponds to
the calibration of the optical fiber, for a certain wavelength, is stored in the flash
memory and loaded at instrument start-up. It is modified with each calibration.
4.5.6 THE CONTROL RAMP
The lamp’s control voltage must be generated from 5 to 12 Volts in steps less
than or equal to 10 mV. This means that no less than 700 (7000/10) control
points are needed.
These points are generated with two devices:
One of them functions as the control range selector (lamp voltage range) and
varies from 0 to 7, and the other as a fine selector (lamp volt. lev.) and varies
from 0 to 127.
This allows 8 linear characteristics to be arranged, which could be “connected”
together to form one unique characteristic of 128x8 = 1024 points. In reality,
however, the start and end voltage in each of the 8 characteristics cannot be
precisely defined and this prevents a true connection.
To therefore prevent gaps between one characteristic and another, the 8
characteristics are generated so that the adjoining characteristics partially
overlap each other (see fig. A):
volt
a)
127
127
0
48
0
0
29
4.0
0
43
4.0
0
37
8.0
0
38
8.0
0
41
12.0
0
31
12.0
0
volt
b)
As can be seen in figure B), the passage from one characteristic to the next
takes place, increasing the range and restarting the count from 0, when the
connection point with the next characteristic is reached. This gives a lesser
number of resolution points since the characteristics no longer start from 0, but
instead from the value corresponding to the intersection and indicated in the
figure.
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This provides a true characteristic equal to that in fig. C, where there is a virtual
counter that lays out 749 horizontal points [ 127 + (127-31) + (127 – 41) +
(127 – 38) + (127 – 37) + (127 - 43) + (127 – 29) + (127 – 48) for scanning
voltages from 5 to 12 Volts.
volt
12.0
8.0
0
784
4.0
The measurement of this characteristic is done automatically for a certain
instrument.
A report is printed at the end.
4.5.7 REPORT OF THE VIRTUAL RAMP
Each ramp segment represents a range (0, 1, …,7). Each range has a starting
point, which is not 0, but depends on the intersection point with the ramp (the
range) that precedes it. This number of points, which is lost in the calculation of
the totalramp, is reported as the start index. The first val, on the other hand,
indicates the A/D conversion value of the lamp voltage in correspondence with
the start index.
first value
0
start index
The voltage on the lamp can be determined using the following formula:
Vlamp = 4.3 * 4000 * first value / 1024
An example of a virtual ramp report is the following:
Range…0
first value 333
start index 0
Range…1
first value 389
start index 19
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Range…2
first value 449
start index 26
Range…3
first value 503
start index 17
Range…4
first value 562
start index 19
Range…5
first value 622
start index 26
Range…6
first value 677
start index 12
Range…7
first value 740
start index 28
Nramp 773
FVD
407
SIT
147
V-: 5212.99
V+ 12833
The criteria for evaluating this report is the following:
-
first value for range0
values from 310 to 330
-
first value for range7
values from 710 to 740
-
each individual value of the start indexes values from 5 to 40
-
Nramp
from 700 to 800
-
FVD
400±15
-
SIT
<200
-
5100.00 >V- > 5000.00
-
13000.00 > V+ > 12500.00
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4.5.8 CONTROL WINDOW
Controlling the calibration of the optical channels is done with the commands and
indications found on the calibration window.
Vir ramp:
starts the control procedure for the virtual ramp and prints the report
Dark
controls the offset values and reports them in the dark column
Light
starts the adjustment of the light emission at a value of 1000 dgt for each channel
using an empty cuvette
The following data are reported:
the transmittance (Air) the virtual ramp value (Vir)
the voltage control value inside the range (Val)
The selected range (Ran)
The dark value (Dark)
Calib:
automatically starts the Dark and Light procedures, one after the other.
Check: performs a complete reading of the optical channels using the selected parameters
Print:
Prints the calibration report displayed on the screen
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4.6 OPTICAL UNIT TESTING PROCEDURE
Note: The test using the controls available on the CHORUS TRIO should be performed without
connecting to Chorus manager trio.
4.6.1 SETTING OF THE OPTICAL FILTER OFFSET
The Settings / Parameters / Mechanical calibration / Optical filter offset
parameter checks the exact position of the filter at the centre of the light ray
outlet hole.
The value of this parameter is determined in the pre-testing phase and must be
included in the unit’s test report (RdC).
If the exactness of this parameter needs to be checked, adjust it as follows:
1. disassemble the lamp unit
2. launch the command Commands / Hardware controls / Motors / Filter
3. the filter carriage moves outwards until the end stroke sensor is reached. In
this state, the absolute sensor is to be positioned, but to perfectly centre the
filters in the outlet hole, the carriage must return within a certain number of
steps (adjustment parameter).
4. The number of steps is determined by trial through the Commands /
Hardware controls / Motors / Filter command, setting the Position and
the Speed value
5. The parameter is defined when the circle of the filter is concentric with that of
the hole. The parameter must be reported in the parameters table.
When finished, launch the reset command, recheck the position and reassemble
the unit.
4.6.2 OPTICAL CHANNELS
The optical interleavers mounted on the instrument must have already been
tested and supplied with a testing document (RdC).
4.6.3 CHECKING OF THE LAMP’SCONTROL VOLTAGE
1. Open the Start / Utility / Service / Calib window on the Chorus trio
2. Select 650 nm filter
3. Give the Vir ramp command
To view the virtual ramp, the instrument needs to be connected to the PC. With
Chorus manager click on Settings /Parameters
The ramp values will appear in the Optical Calibration window.
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There is a steady increase of the broken line between the value of 5 Volts and
that of 12 V DC.
To further check the control voltage, a voltmeter can be put on the lamp’s
voltage (without disconnecting the lamp) and setting the value using the
following procedure:
1. launch the command Commands / Hardware controls / Lamp
2. set Range to 0 and Light to 0
a voltage reading within the range: 5 Vdc ± 0.2V must be obtained
3. set Range to 7 and Light to 127
a voltage reading within the range: 12Vdc 13Vdc must be obtained
4.6.4 CALIBRATING THE OFFSET (DARK READING)
Staying in the Start / Utility / Service / Calib, window
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cover the instrument with a sheet so that it cannot be influenced by ambient
light (or reducing the ambient light), and activate the Dark command
After resetting the plate, the instrument begins to assess the values (lower). The
first row (yellow) shows the programming digit while that below shows the value
read.
The obtained values must be:
-
>0 for the programming digits
-
between 20 ±5 digit for the off-set value
4.6.5 CALIBRATING THE LIGHT
One must make sure that the offset has been calibrated before adjusting the
light.
4.6.5.1
Calibration of the 650 nm filter
1. Go to the Start / Service / Reset / Calib window, with the 650 nm filter and
covering the instrument with a sheet so that it cannot be influenced by
ambient light (or reducing the ambient light), activate the Light command
The system measures the lamp’s control current channel by channel in order
to obtain a reading in air near 1000 dgt
This must occur with a range value not higher than 1 for each channel
2. Then send the Check command and read the final values, which must stay
within 1000 ± 10
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4.6.6 CALIBRATION CURVE
The CC test must be done by the Chorus trio. Open the Service window (Start /
Utility/ Service).
Note: the solutions are kept at room temperature (25°) and the temperature control system should be
deactivated for the tests
1. Prepare seven LAB type strips, with the 2 reaction wells correctly inserted and
both filled with the following solutions:
strip no. 1
100 µl
solution 1:512
strip no. 2
100 µl
solution 1:256
strip no. 3
100 µl
solution 1:128
strip no. 4
100 µl
solution 1:64
strip no. 5
100 µl
solution 1:32
strip no. 6
100 µl
solution 1:16
and place them in the first seven positions in the carousel.
2. Give the TRead command as in the figure and wait for the cycle to finish.
Select the filter with which to make the measurement.
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Pressing Auto starts an automatic procedure that checks the presence of the
strips. The Edit button can be used to modify the number of strips present using
the keyboard.
3. the following screen appears after the reading
4. Take the data with a spreadsheet management program and enter the x-axis
values as shown in the figure, then go to the graphs of the curves.
Ch1
Ch2
Ch3
Ch4
Ch5
Ch6
86
108
89
97
92
93
0.003906 156
167
161
154
165
151
0.007813 275
275
258
262
262
264
0.015625 520
520
524
523
524
517
0.03125 929
929
986
945
994
944
0.001953
0.0625 1542 1542 1668 1592 1647 1558
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OTTICA 014
2500
2000
1500
1000
500
0
0
0,01
0,02
0,03
0,04
0,05
0,06
0,07
An assessment criterion of the calibration curve data is the following:
•
The initial values of the characteristics must be 80 < Vi < 110
•
The final values of the characteristics must be 1400 < Vi < 1700
Warning:The values can change if the calibration solutions are changed or if they were improperly
stored
Note: the test certificate is composed of the graph with the table.
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5 WASHING UNIT
5.1 HYDRAULIC WASH CIRCUIT
The part of the hydraulic circuit that supervises the washing of the wells is shown
in the following diagram:
CS sen.
(1-4)
closure
cap
ev10
pp7-1
21
pp7-2
pp2
ev2
S1
pp6
closure
cap
Buff. Sol.
Autoimm.
ev11
pp4-1
25
ev1
pp4-2
ev12
pp1
S2
B1S sen
(1-4)
Buff. Sol.
Infective
pp5
ev6
28
p1
B2S sen
(1-4)
Wasting
ev1:
solenoid valve for exchange between the autoimmunity washing-buffer solution and the air
ev2:
solenoid valve for exchange between the ev12 selection and the cleaning solution
ev6:
solenoid valve for exchange between the infective washing-buffer solution and the air
ev10:
solenoid valve for stopping the flow of washer #1
ev11:
solenoid valve for stopping the flow of washer #2
ev12:
solenoid valve for the exchange of the buffer tanks
pp1:
supply pump in the needles of washer #2
pp2:
supply pump in the needles of washer #1
pp4:
aspiration pump from the needles of washer #2
pp5:
aspiration pump from the collection basin of washer #2
pp6:
aspiration pump from the collection basin of washer #1
pp7:
aspiration pump from the needles of washer #1
p1:
aspiration pump from the needles of washer #3
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5.2 TANK PROBES
The instrument has three probes (infective washing-buffer solution,
autoimmunity washing-buffer solution and cleaning solution) whose function,
besides that of withdrawal, is to check and report the amount of liquid present in
the tank in which they are inserted.
Each probe has two terminals:
•
a plastic tube with relative connector (6) for the drawing up of the liquid,
highlighted with a blue, green or white band (8)
•
an electric wire, with relative connector (7), for detecting the liquid level
which are attached to the respective connectors located in the instrument’s tank
chamber
Warning: Each probe must always be used with the same solution in order to prevent
cross contaminations.
Use the probe with the blue band for the tank with the Infective Washing Buffer, that
with the green band for the tank with the Autoimmunity Washing Buffer and that with the
white band for the Cleaning solution, following the color-coding indicated on the
instrument’s connectors
Once connected, the probe takes the liquid from the spout and puts it into the
hydraulic circuit through the fitting.
The four sensors, located on the probe rod, report the liquid level in the tank.
The sensor signals are sent to the instrument through the electric connector.
1. withdrawal spout
7
2. 0% level sensor
3. 25% level sensor
1
2
3
4
5
6
8
4. 50% level sensor
5. 75% level sensor
6. hydraulic fitting
7. electric connector
8. band
The levels managed by the Chorus trio are:
0%:
tank empty – the instrument stops any ongoing cycle and reports a
warning
25%:
tank almost empty – if the buffer solution drops below this level, a
warning will be generated during the initial check and the cycle
cannot be started
50%:
if the washing solution drops below this level, a warning will be
generated during the initial check and the cycle cannot be started
75%:
first control level
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5.3 THE WASHER
CHSMIT30
The washer is a device that is designed to move the wash needles up and down
in the two reaction wells (no. 5 and no. 6) to repeatedly draw up or dispense the
buffer
solution
with
which
the
two
wells
are
“cleaned”.
The wash needle is composed of a pair of stainless steel tubes: one for washing
and one for aspirating. The aspiration tube is straight and works to reach the
bottom of the well to ensure the complete aspiration of the liquid present. The
upper part is shaped in order to stop the tube.
The dispensing tube is of a small diameter, has a tapered end and never touches
the liquid in the well. The upper end is bent to separate the two tubes at their
connection point. The upper part is shaped to stop the tube.
Both tubes have a teflon tip for better runoff of water particles. The two tubes
are joined together by a case, which slide inside an axial bearing, while the
needle is held in the upward movement by a spring. To ensure that the needle
adheres to the bottom of the well, the needle drops below the level of the well
bottom, making the spring intervene. The needle must therefore slide vertically,
which
occurs
along
the
axial
bearing.
The needles, with the parts described above, are positioned in the needle block,
which is made up of two parts fastened together by a central screw.
The needle block is mounted on a bracket that is connected to the slider which
moves up and down along the slide. The bracket is integrated with a toothed
rack and therefore the rotation of the motor, with flush fit shaft pinion, produces
the
vertical
movement
of
the
needle.
A magnetic sensor mounted on an electronic board, and relative magnets,
mounted on a connecting rod is used to set the vertical position.
A U-shaped aluminium bracket supports all the parts and the base of this bracket
is fixed to the upper surface of the instrument.
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1
A
left
right
sezione
A- A
2
10
9
11
12
3
13
14
4
5
8
15
7
6
A
16
RIGHT
FRONT
LEFT
17
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
bracket
connecting bracket
needles
needle-holding block
slider
slide
toothed rack
pinion
motor
position sensor
aspiration tube
supply tube
tube casing
spring
axial bearing
tapering
Teflon covering
5.4 THE DRYING STATION
As can be seen from the hydraulic circuit and from the structure, after the two
washing stations (washers #1 and #2) there is a third station for drying (in pos.
no. 28).
The drying station is a device that can drop an unifilar needle into either of the
reaction wells in order to remove the contents.
The mechanics are the same as with the washer
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5.5 COLLECTION WELLS
As can be seen from the hydraulic circuit and the structure, the instrument has
three washing stations, or rather two washing stations (washer #1 in pos. no. 21
and washer #2 in pos. no. 25) and a third station for drying (washer #3 in pos.
no. 28).
A washing station is a device that can drop a bifilar needle into either of the
reaction cuvettes. The needle is composed of one larger diameter straight needle
and one smaller diameter needle that is tapered at the end. The two tips are
Teflon coated in order to prevent the formation of drops.
The straight needle is used to remove the liquid in the well. The tapered needle
is used to dispense washing buffer.
The two needles move up and down together and the descent into the bottom of
the well is dampened by a spring.
1
1. straight needle
2. tapered needle
3. sensors
2
3
The diagram shows the section below the strip introduction tray.
As can be seen, there is a collection basin. When there are no strips in the tray,
the contents of the needles are unloaded in the well during the priming or
cleaning of the needles.
The well is drained by pump pp6, if we are in position 21 or by pump pp5 if we
are in position 25.
For safety purposes, each well is equipped with a pair of sensors that can report
any overflowing of liquid.
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5.6 WASHING PROCEDURE
The sequences described below show how the pair of cuvettes are washed in
correspondence with Washer #1 (pos. 21) and Washer #2 (pos. 25), whether one or
both of the cuvettes are filled. The duration of the operation is equal to the
standstill time during the step (approx. 7-8 sec).
We will describe the operation of Washer #1:
1
2
3
5
6
7
4
1. The needles are in the resting position
2. Spring dampened descent into cuvette, with simultaneous aspiration of the liquid through
pump pp7. At the bottom of the descent, the cuvettes are dry and the needles remain in the
cuvette for approximately 3 seconds.
3. Raising of the needles and successive dispensing of the washing buffer. Dispensing is done
by pump pp2, with ev1 and ev2 off.
4. Spring dampened descent into cuvette, with simultaneous aspiration of the liquid through
pump pp7. At the bottom of the descent, the cuvettes are dry.
5. Raising of the needles and successive dispensing of the washing buffer. Dispensing is done
by pump pp2, with ev1 and ev2 off.
6. Spring dampened descent into the well, with simultaneous aspiration of the liquid through
pump pp7. At the bottom of the descent, the cuvettes are dry.
7. Return to the resting position.
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5.7 WASHING CIRCUIT TESTING PROCEDURE
Have available:
•
one tank with blue coloured conductive solution as buffer no. 1
•
remove the strips from the carousel and reset the tray
5.7.1 SUPPLYING OF BUFFERFROM WASHER #1
1. From Chorus manager trio deactivate the pumps and solenoid valves and
disable the motors (Disable all button)
2. Lower washer no. 21 to the level of the surface
3. Turn on Pp2
(Pumps / Pp2)
check that the blue coloured water begins to flow through the tubes and goes
into the needles of washer #21.
Lower the washer to the bottom
4. When the liquid touches the contacts of the basin, pump Pp2 must stop and
pump Pp6 must switch on to empty the well
5. Retry activating Pp2 again
6. again deactivate the pumps and solenoid valves and disable the motors
5.7.2 SUPPLYING OF BUFFER FROM WASHER #2
1. From Chorus manager trio deactivate the pumps and solenoid valves and
disable the motors (Disable all button)
2. Lower washer #25 to the level of the surface
3. Turn on Pp1
(Pumps / Pp1)
check that the blue coloured water begins to flow through the tubes and goes
into the needles of washer #25.
Lower the washer to the bottom
4. When the liquid touches the contacts of the basin, pump Pp2 must stop and
pump Pp6 must switch on to empty the well
5. Retry activating Pp1 again
6. again deactivate the pumps and solenoid valves and disable the motors
5.7.3 WASHING OF THE PIPINGFOR WASHER #1
1. From Chorus manager trio deactivate the pumps and solenoid valves and
disable the motors (Disable all button)
2. Lower washer #21 to the level of the surface
3. Activate SV2 (Valves / SV2)
4. Turn on Pp2 (Pumps / Pp2)
- Check that the clean water begins to flow through and replace the blue
coloured
water.
When the basin is full, pump Pp2 must stop and pump Pp6 must switch on to
empty the well
5. Retry activating Pp2 again until the tubes with coloured water are empty.
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6. again deactivate the pumps and solenoid valves and disable the motors
5.7.4 WASHING OF THE PIPING FOR WASHER #2
1. From Chorus manager trio deactivate the pumps and solenoid valves and
disable the motors (Disable all button)
2. Lower washer #25 to the level of the surface
3. Activate SV2 (Valves / SV2)
4. Turn on Pp1 (Pumps / Pp1)
- Check that the clean water begins to flow through and replace the blue
coloured
water.
- When the basin is full, pump Pp1 must stop and pump Pp5 must switch on
to empty the well
5. Retry activating Pp1 again until the tubes with coloured water are empty and
6. again deactivate the pumps and solenoid valves and disable the motors
5.7.5 ASPIRATION AT DRYING STATION #3
1. From WinChorus trio deactivate the pumps and solenoid valves and disable
the motors (Disable all button)
2. Put a strip with two cuvettes filled with liquid under the drying station position
and lower the needles until they are inside the cuvettes.
3. Activate Pp1 for a few seconds and check that the liquid is completely
aspirated
4. again deactivate the pumps and solenoid valves and disable the motors
5.8 CONTROL PARAMETERS
5.8.1 FILLING LEVEL OF WASHER #1
This is the number of steps needed to position the aspiration needle near the
upper edge of wells 5,6. The needle must be inside the wells at approximately
2mm from the upper edge, so that any excess liquid can be aspirated.
The procedure to calculate this parameter is the following:
1. Send the tray reset command using the Tray key and then click on Reset
2. Insert a clean strip in the tray and rotate it so that the strip is in line with
washer #1
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3. Select the Macro / Check washers / Washer #1 command and give the Start
command
4. Remove the strip and check that the liquid level in wells 5,6 is approximately
2mm from the edge
Set the value found in the parameters table Settings / Hardware parameters /
Mechanical calibration / Washer #1 bottom level
The default value is 50
5.8.2 FILLING LEVEL OF WASHER #2
This is the number of steps needed to position the aspiration needle near the
upper edge of wells 5,6. The needle must be inside the wells at approximately
2mm from the upper edge, so that any excess liquid can be aspirated.
The procedure to calculate this parameter is the following:
1. Send the tray reset command using the Tray key and then click on Reset
2. Insert a clean strip in the tray and rotate it so that the strip is in line with
washer #2
3. Select the command Macro / Check washers / Washer #2 and give the Start
command
4. Remove the strip and check that the liquid level in wells 5,6 is approximately
2mm from the edge
Set the value found in the parameters table Settings / Hardware parameters /
Mechanical calibration / Washer #2 bottom level
The default value is 50
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6 THE DRAIN CIRCUIT
The part of the hydraulic circuit that supervises the discharge of the liquids is
shown in the diagram below.
The circuit is designed so that the Chorus trio can be connected to a collection
tank or to a central drain.
main waste
well
S3
waste full
sensor
safety
return tube
pp8
S4
Waste warning
sensor
Waste tank
The following devices intervene:
pp8:
pump for main waste well
6.1 THE MAIN WASTE WELL
11
10
3
9
2
1
4
8
5
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
7
aspiration pump a
waste intake (blue)
liquids intake
pump expulsion
waste outlet (red)
outlet line
outlet closed return
connector
warning sensor S3
error sensor S4
outlet closed return
inlet
wash well drain
6
The waste well is the device that regulates the evacuation of the liquids not
needed by the Chorus trio.
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The liquids coming from the waste collection tube of the pumps reach the well
through the fitting, while the liquids coming from the dispenser wells arrive from
the hole in the cover through a tube.
Peristaltic pump pp8 takes in the liquid collected from the fitting and sends it
towards the red outlet, connected to the well by the junction, through the outlet
line; the connection to the blue outlet is ensured by the fitting.
Besides collecting and expelling waste liquids, the well monitors the proper
functioning of the circuit through the use of an auxiliary waste outlet well,
connected to the outlet line by the fitting and to a series of level sensors.
Since the hydraulic circuit is directly controlled by the waste well, the use of
external level sensors are therefore not necessary.
6.1.1 NORMAL OPERATION
S3
S4
pp8
The liquids to be discarded are sent through one inlet (waste inlet).
When the liquid reaches the level of the warning sensor (WS), drain pump pp8 is
immediately activated and the liquid is evacuated through the output indicated
with the colour red on the rear panel.
The liquid flows toward the outlet without entering in the recirculation circuit, as
the diameter of the tube for this circuit is much smaller.
The intervention of the warning sensor is not reported during normal operation.
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6.1.2 OBSTRUCTED DRAIN
S3
S4
pp8
When the drain is obstructed or cannot be used, for example if the tank is not
connected, the liquid pushed by pump pp8 is sent into the recirculation circuit
and then returns into the waste well, raising the liquid level inside the well.
The main waste well therefore begins to fill.
There is an error sensor (ES) in the well placed at the maximum allowable filling
level.
The instrument stops and a fatal error is generated when the sensor is reached.
If the obstructed drain is resolved before the ES is reached, the liquid is
evacuated as normal by pump pp8.
6.1.3 FULL WASTE TANK
S3
S4
pp8
When the waste tank is full and the cap has been screwed on correctly in order
to seal the tank, the waste liquid will come back into the instrument through the
blue pathway (safety input), filling the auxiliary well until the error sensor is tripped,
at which time the instrument will generate a fatal error.
To summarize:
WS activated = drain problems
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6.1.4 FAULTY DRAIN PUMP
S3
S4
pp8
When drain pump pp8 has broken down, the liquid continues to flow into the
waste well, initially activating the warning sensor (WS), and then the error sensor
when the well is full. A fatal error is generated at this point.
To summarize:
WS activated = drain problems
6.2 WASTE CIRCUIT TESTING PROCEDURE
6.2.1 CHECKING OF WASTE LEVEL WARNING SENSOR S3
1. Connect the water tank with saline solution
2. Interconnect the two outlets of the waste tubes
3. Activate SV4 (Valves / SV4) and Pp3 (Pumps / Pp3)
4. Check that the flow of water comes out of UGEL 1.
5. Activate SV5 (Valves / SV5)
6. Check that the flow of water comes out of UGEL 2.
7. Let the water rise up to the level of the warning sensor (Waste well half full)
S4
8. Check that the Warning alarm activates (Waste well half full) and that PP8
drains the well
6.2.2 CHECKING OF ERROR SENSORAND WASTE LEVEL
1. Continue filling until the water that comes out of the RED pathway returns
into the well through the BLUE pathway.
2. Wait
until
the
waste
level
error
sensor
S3
is
while Pp8 continues to run, the Waste well full alarm must activate
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3. Disconnect the waste line from the RED PATHWAY and insert it in the tank
and wait for the well to empty and Pp8 to stop.
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7 TEMPERATURECONTROL
7.1 TEMPERATURE CONTROL OF THE INSTRUMENT
The diagram of the temperature control devices on the instrument is shown in
the figure.
First of all, the instrument is considered to be “closed” from a thermal point of
view, since the container that houses it is thermally insulated. It should be noted
that there are heat sources inside the instrument heater, power supply, lamp, motor drivers,
motors, etc...).
The following are found inside the instrument to regulate the temperature:

cooling fans that take in the air inside the instrument

air vents with dust filter, where air is drawn in for internal cooling

internal temperature sensor.
2
1.
2.
3.
4.
fans
protective housing
temperature sensor
air vents
1
3
4
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7.2 MEASURING CHAMBER
One of the specifications for performing a test is the temperature at which the
reaction must occur. The operating temperature range can vary from 25°C to
40°C and is automatically programmed before every cycle.
Heating occurs by the emission of hot air into the heat chamber (where the tray
rotates) by means of a heater, where a fan directs hot air into the measuring
chamber.
The hot air also heats the upper part of the tray for the strips and consequently
the strips themselves.
The emission of hot air is regulated by a system that uses the temperature
sensor inside the chamber.
The tray must get to temperature before the instrument can be used.
At start-up, a wait of up to 25’-30’ may be required to allow the heat chamber
and the tray to get up to temperature (typically 38°C) if the machine is cold.
6
4
3
1
5
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1.
2.
3.
4.
5.
6.
lower base
heater
temperature sensor
measuring chamber walls
heat chamber (internal)
tray
2
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7.3 HEATER
7
6
5
1
2
3
4
5
6
Fan
Housing
Heating coil
Dust filter
Flange
Fastening holes
2
3
4
1
The heater is the device that works to keep the temperature in the heat chamber
stable.
It is composed of a coil wrapped around the heating unit and encased by a PVC
cover; a 50x50 mm wide fan with a 24 V DC power supply is used to transfer the
heat into the chamber. This hot air then passes into the heat chamber through
the heater cylinder.
The impurity filter is positioned between the fan and the protective grill.
The heater is mounted on the upper plate of the Chorus trio by means of the 2
fastening holes in the support flange.
7.3.1 ELECTRICAL PROPERTIES OF THE HEATER
Rated voltage: 24V DC
Resistance value 3 ohms
Power output: 192 W
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7.4 CYCLE TEMPERATURE
The cycle temperature is determined through the operating temperature of the
tests identified on the tray.
If the operating temperature of the tests is not homogeneous:
 the cycle is stopped
 the incongruent strips are indicated on the display
The cycle will only restart if there are tests in the identification that have the
same operating temperature.
If the temperature of the tray is not at the required temperature, the cycle will
only restart when the system returns to temperature.
7.5 STAND-BY TEMPERATURE
When the cycle is finished, the temperature of the tray is regulated by the standby value.
The stand-by temperature, normally set at 30°C, prevents the tray from cooling
and reduces the successive heating time of the cuvettes.
7.6 PROCEDURE FOR TESTING THE TEMPERATURE CONTROL SYSTEM
7.6.1 CHECKING OF THE TEMPERATURE SENSOR
1. Remove the protective housing
2. Safely heat the instrument’s temperature sensor and check that the rear fans
start when 35° is exceeded.
The temperature inside the instrument is checked through Commands /
Hardware controls / Thermometers / Instruments and must be below 35° in
all seasons and when the ambient temperatures is < 30°C.
7.6.2 CHAMBER TEMPERATURE CONTROL
The following is required to start this test:
-
The instrument,
temperature
complete
with
housing,
switched
off
and
at
room
-
a cuvette in position 1 with 300µl of water in well 6 and a thermometer.
-
The Settings / Parameters / General settings / Idle temperature
parameter must be set at 38°
Switch on the instrument and check that the Strip Temp temperature in the
Start \ Service \ Info window rises to 38° within 30’.
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7.6.3 PROGRAMMING THE STAND-BY TEMPERATURE
CHSMIT30
The stand-by temperature, or rather the temperature when the instrument is at
idle, is set in this phase.
The temperature must be checked in order to avoid long waits at the start of a
new cycle so that the instrument can get up to the operating temperature, which
is typically near 38°.
It is recommended that the control parameter be set to 30°.
The heating test must be performed with the protective housing of the chamber
inserted.
1. The programming of the stand-by temperature is done from the parameters:
Settings / Parameters / General settings / Idle temperature
2. The setting of the temperature influences the time. The default value is 30°.
Check that the internal thermometer reads 30°
3. Later, set the parameter to 25°C and check that the system cools and
remains at 25°
4. Then set it to 40° and check that the system heats and remains at 40°
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8 SPEAKER-PRINTER DISPLAY
8.1 DISPLAY
2
1
3
7
4
1. Display
2. Touch screen
display area
3. Display lamp cable
4. Display data cable
5. Inverter
6. TSI- Touch Screen
Interface board
7. Touch screen cable
6
5
INVC186
HITACHI
CN1
CN2
The Chorus trio is equipped with a liquid crystal display touch screen; this allows
you to interact with the instrument by simply touching the screen with your
fingers.
The touch screen interface board adapts the flat cable that comes out of the
touch screen to the cable that will then go to the CPU 2010
The inverter board instead supplies power to the back-lighting lamp for the
display.
8.2 THE SPEAKER
The speaker is a device that emits sound signals for instrument errors that are
modulated according to the error detected:
-
Recoverable errors - high-pitched intermittent signal
-
Warnings - low-pitched intermittent signal
-
Fatal errors - Two-tone signal that cannot be deactivated
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Chorus trio – SERVICE MANUAL
CHSMIT30
8.3 THE PRINTER
1. Roller
The Chorus trio is fitted with a thermal printer that reports the results of the
various instrument tests and the results of previous tests onto paper.
Thermal paper must be used for this type of printer and is supplied in rolls. As
can be seen in the figure above, to insert the paper one must raise lever 2, place
the edge of the paper under roller 1, let it pass under the roller until it comes out
above, then lower the lever.
8.3.1 PRINTER TESTING
8.3.1.1
Loading the paper
From the Chorus manager window Commands / Hardware controls / Sensors.
1. Raise the lever, remove the paper and lower it
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Chorus trio – SERVICE MANUAL
2. Check that the signals below are in the following state:
-
Out of paper sensor is on (Printer carriage open)
-
Printer malfunction sensor is off (Printer Parer Ok).
CHSMIT30
3. Manually introduce the paper until the roller begins to move it, then check
that the signals are in the following state:
-
Out of paper sensor is off
-
Printer malfunction sensor is off
4. Raise the lever and check that the signals are in the following state:
 Out of paper sensor is off
 Printer malfunction sensor is on
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Chorus trio – SERVICE MANUAL
9
CHSMIT30
ELECTRONIC PARTS
The main electronic boards of the Chorus trio are described in this chapter.
9.1 GENERAL MAP
Fig. 9.1.1 shows the overall diagram of the instrument with the electronic boards
and all the devices that are connected to it.
As can be seen, the CPU 2010 is the board that controls all the other boards
(Low Power, Connectors, Power Supply), all the motors (Wash1-3, Syringe, Tray,
Disp1-2, X Carriage, Filter and TSD), the two thermostats (Instrument and
Chamber), the optical channels, the LCD display (Touch Screen & Display), the
printer
and
the
Internal
Bar
Code
Reader.
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Chorus trio – SERVICE MANUAL
CHSMIT30
Optical Channels
Sheet 2
Buffer #1
Buffer #2
Buffer #3
Buffer #4
Inverter
Rear Fan
Washing Well #2
Washing Well #1
Waste Error
Waste Warning Level
Waste Output Closed
Printer
Touch Screen
& Display
TSI Board
Instrument
Thermostat
Chamber
Thermostat
Sheet 1
Cleaning Solution #1
Cleaning Solution #2
Cleaning Solution #3
Cleaning Solution #4
WASH #3
SYRINGE
TRAY
DISP #1
DISP #2
X CARRIAGE
FILTER
CPU 2010 Board
DRIVER 2010 Board
WASH #2
LOW POWER
WASH #1
LID
SPS
Dispenser #1
Dispenser #2
Tray Lock Device
( TLD )
SID
P1
PP8
EV10
EV5
PP1
PP2
EV11
TSD
EV6
Heater
EV4
EV2
Internal Bar Code Reader
PP4-1
PP4-2
Speaker
EV1
Connectors
PP3
POWER SUPPLY
PP5
EV7
PP6
EV3
PP7-1
EV8
PP7-2
EV9
Sheet 3
POWER IN
Output Connectors
Version 3.0 – Revision 0 - 29/11/12
81 of 151
Sheet 4
Chorus trio – SERVICE MANUAL
SYRINGE
TRAY
Fig. 9.1.1 - Instrument Map Sheet 2 of 5
Version 3.0 – Revision 0 - 29/11/12
82 of 151
CPU 2010 Board
WASH #3
DRIVER 2010 Board
WASH #2
Inverter
Optical Channels
Rear Fan
WASH #1
Touch Screen
& Display
TSI Board
Chamber
Thermostat
Instrument
Thermostat
Sheet 1
CHSMIT30
Chorus trio – SERVICE MANUAL
CHSMIT30
Sheet 2
Washing Well #2
Washing Well #1
Waste Error
Waste Warning Level
Printer
LOW POWER
CPU 2010 Board
Buffer #1
Buffer #2
Buffer #3
Buffer #4
LID
SPS
Dispenser #1
Dispenser #2
Tray Lock Device
( TLD )
SID
P1
PP8
Fig. 9.1.1 - Instrument Map Sheet 3 of 5
Version 3.0 – Revision 0 - 29/11/12
Cleaning Solution #1
Cleaning Solution #2
Cleaning Solution #3
Cleaning Solution #4
83 of 151
DRIVER 2010 Board
Chorus trio – SERVICE MANUAL
Sheet 3
DISP #1
DISP #2
DISPENSATION
CARRIAGE
FILTER
CPU 2010 Board
CHSMIT30
TSD
Internal Bar Code Reader
MODEM BOARD
Speaker
POWER SUPPLY
Output Connectors
Fig. 9.1.1 - Instrument Map Sheet 4 of 5
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84 of 151
Chorus trio – SERVICE MANUAL
CHSMIT30
Sheet 4
PP8
EV10
CPU 2010 Board
Heater
LOW POWER
EV5
POWER SUPPLY
Fig. 9.1.1 - Instrument Map Sheet 5 of 5
Version 3.0 – Revision 0 - 29/11/12
85 of 151
PP2
EV11
EV12
EV4
EV2
EV1
PP3
PP4-1
PP4-2
PP5
EV7
PP6
EV3
PP7-1
EV8
PP7-2
EV9
POWER IN
PP1
Chorus trio – SERVICE MANUAL
CHSMIT30
9.2 CPU 2010 BOARD AND DRIVER 2010 BOARD
The Chorus trio’s central processing unit was divided into two boards: CPU2010
and DRIVER2010.
The CPU 2010 is the board that controls all the functions of the Chorus trio.
All the functions of the Chorus trio refer to it, from the moving of the motors, to
the management of the hydraulics, to the acquiring of analog signals coming
from the optical sensors, to viewing on the LCD display, etc.
The board is found on the left side, looking at the instrument from the rear,
under the display/printer unit, and is fixed to the frame by 4 screws in its 4
corners (see Fig. 9.2.1).
The DRIVER 2010 board is mounted alongside the CPU 2010 and a majority of
the connectors are mounted on it. In particular are the connectors for the motors
and the thermometers
9.2.1 POWER SUPPLY
The CPU 2010 board is powered directly from the Power Supply Unit through
connector CN11 (+5V,+12V,-12V) and cable CAB 536. The voltages are reported
by means of 4 LEDs ( +5V - DL5, +12V - DL6, -12V - DL7, +24V - DL8). The
DRIVER 2010 board is powered through connector CN11 (+24V).
Fig. 9.2.1 Location of the CPU 2010 and DRIVER 2010 inside the instrument
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86 of 151
Chorus trio – SERVICE MANUAL
CHSMIT30
TP23
J2
CN2
C5
1
C12
C9
CN3
L8
C25
C22
C19
L157
L156
L15
L154
C312
L155
C29
CN18
CN17
1
C44
C43
CN6
R51
R60
DL1
C125
BNX1
C132
C139
BT1
C128
TP26
X2
L49
CN9
R48
C122
R59 C124
1
C157
CN10
C146
IC18
C142
IC17
L53
C147
DL2 DL3 DL4
K1
L54
C154
C156
C160
C159
L55
L56
R83
L57
L58
R186
L138
R187 DL6
CN14
C164
C163
L64
R84
TP15
DL5
CN11
R82
L63
R184
R185
C290
C289
L139
C291
L140
C293
L141
C294
C295
L142
L69
L68
L135
DZ5
D4
L70
C283
C284
TP16
C161
DZ4
C165
D3
C169
L65
R85
L71
C167
IC50
R79
R78
R77
L143
C292
R81
C158
C151
Fig. 9.2.2 Layout and connections - complete view
87 of 151
CN7
C101
C281
IC13
JP1
TP14
C166
C102
R47
L51
R70
L62
C162
L43
C105
L44
C109
C112
L47
L48
TP22
C282 L42
C108
C118
DZ3
R56
DZ2
R67
R68
R71
C145
R73
R75
C153
L133
R41
L37
C94
L38
C98
C99
L40
C103
L41
IC11
L132
C107
R45
R44
DZ1
L45
C133
R74
1
C91
IC10
IC49
C285
TP28
R46
L46 C111
R50
IC12
R53
C117
C113 TP27
C121
R57 R58
R55 IC15
R61
R62 R63
L50
R65
C131
C135
L52
C130
C134
IC16
C149
R72
L66
L67
CN12
C83
L32
L36
C89
C88
C87
R36
R35
R34
R33
R32
R31
C86
L39
C97
C96
C120
IC14
R66
C140
D1
FA3
L35
C90
L136
L134
C286
R43 R183
TP10
R76
C155
R80
C54
R25
C79
C119
D2
CN14
C83
L41
L61
L60
L40
C287
TP100
C86
L44
C82
CN13
MX1
IC19
L59
L30
R27
L34
R38
C85
TP101
IC19
CN11
F1
2 1
CN12
C67
R92
R84
R78
DL1
C116
R52
C152
IC4
C55
IC8
C95
X1
L36
C150
CN17
R64
R72
C68
R69
C143
L22
C53
L21
C52
R19
C51
R18
C50
C49
C45
C42
C93
C138
L32
R100
R46
R58
R56
C62
L33
C288 L137
R54
R64
C137
C136
C141
L37
C69
IC17
PAD10
R26
R57
C49
R45
R17
PAD9
R35 R36
CN10
C39
C50
Version 3.0 – Revision 0 - 29/11/12
Q1
R2
R7
R6
C4
C3
C2
L3
R5
R4
C8
C41
C40
L29
C81
CN20
C66
R91
R96
R77
C127
R55
IC10
Sheet 3
L29
C55
C74
C82
TP13
C115
C144
C148
C71
C77
L31
L30
R28
TP4
R49
C67
C76
C75
J4
R26
R42TP2
TP3
TP11
C106
C126
C129
C65
C48
C29
L31
C61
C64
IC6
C68
R40
C123
C84
L43
C56
C73
C80
C81
R30
R39
C114
C80
R16
L23
C48
L20
L23
C66
C100
C85
C61
C38
IC9
Sheet 2
L24
L28
IC9
C110
L42
C79
L39
L35
R15
PAD8
R33 R34
L4
CN19
C60
R54
C58
C20
CN5
C16
IC7
C77 C78
C64
IC13
C57
L2
L3
L12
CN16
C60
C65
R37
C104
L38
L26
C14
C15
CN22
CN18
R61
R59
R71
R14
PAD7
R31 R32
R13
CN9
R25
C34
TP1000
L11
C63
C72
C78
R29
C92
C76
L33
R76
R53
C47
L1
C70 L27
J3
C84
R12
IC12
C37
C28
C39
R103
R21
R23
C69
R24
L34
IC8
C23 C24
C21
C58
C57
C59
L26
C62
CN21
R83
R75
R70
R11
PAD6
R43 R44
C19
CN4
R24
R10
L10
C27
L13
L25
C51 R63
R52
C46
C27
L19
IC5
C59
L21
C36
IC7
C47
C87
L45
R99
C63
L28
R10
CN8
L17
C11
L18
C12
L19
C13
L20
R23
C33
TP24
C37
Q1
R95
R90
R74
R9
PAD5
R29 R30
R51
C45
C26
C20
C26
R15
R17
R69
C35
IC6
CN16
IC16
R106
R8
C18
CN3
R22
C54
R82
C44
C25
L9
IC18
R105
R7
R50
C34
IC5
C11
C10
R8
L25
PAD4
R27 R28
CN7
L13
C8
L14
C9
L15
C10
L16
R21
IC2
C28
IC3
L18
C46
R98
R94
R89
C43
C24
C14
C15
L4
L17
C33
IC4
C1
C38
R108
R104
R107
R88
L27
C56
IC11
R20
C6
R60 R68
R49
IC1
R5 R6
R97
C23
C17
CN2
C6
L11
C7
L12
C42
IC3
PAD3
R41 R42
C5
L2
L6
C16
C17
L7
C18
R9
R11
R12
R13
R14
R16
C32
C35
C36
CN4
IC15
L5
R102
C73
C74
C75
CN15
L22
C52
C13
R87
R81
R73
R67
R3 R4
CN6
PAD2
R39 R40
R48
C32
L10
R101
C70
C71
C72
R86
R80
R66
R62
C41
R19
L9
JS5
L1
C7
CN1
IC14
L8
C22
FA2
J1
R3
IC51
R47
R18
IC2
CN1
1
R189
C4
C31
Sheet 1
JS4
C40
JS3
C3
L24
C53
JS2
R1 R2
C2
L6
L7
JS1
C21
PAD1
R37 R38
L5
R93
R85
R79
R65
IC1
R1
CN15
FA1
C30
C1
C168
FA4
Sheet 4
Chorus trio – SERVICE MANUAL
CHSMIT30
Sheet 1
TP23
IC1
L5
C21
R1 R2
C2
L6
C3
C40
R93
R85
R79
R65
C30
C1
PAD1
R37 R38
Wash #1
CAB511
L24
C53
R47
CN1
IC14
R18
L7
C4
L8
C31
R62
C41
IC2
R87
R81
R73
R67
R3 R4
CN6
IC15
C32
R108
R107
R88
L27
C56
C33
R50
R95
R90
R74
R99
C63
L28
R10
PAD5
R29 R30
R9
C18
88 of 151
R22
R51
Q1
R103
R69
C44
C25
CN16
IC16
R106
R82
C34
IC5
IC18
C54
R105
R8
R21
L25
PAD4
R27 R28
C24
R7
CN7
CN3
L13
C8
L14
C9
L15
C10
L16
R98
R94
R89
C43
IC4
Fig. 9.2.2 Layout and connections - Sheet 1
Version 3.0 – Revision 0 - 29/11/12
R60 R68
R104
R20
Syringe CAB514
Optical Filter
CAB512
R49
IC11
L11
C7
L12
C23
C17
CN2
C6
R102
C73
C74
C75
R97
IC3
L10
PAD3
R41 R42
C5
C42
R5 R6
Wash #3 CAB511
CN15
L22
C52
R19
L9
R101
C70
C71
C72
R48
PAD2
R39 R40
C22
R86
R80
R66
Wash #2 CAB511
C87
L45
Chorus trio – SERVICE MANUAL
Sheet 2
CHSMIT30
Low
To CN1
PowerBoard CAB540
R1
CN15
FA1
CN1
FA2
J1
R189
J2
R3
CN2
IC51
R7
R6
C4
C3
C2
L3
R5
R4
C8
C7
C6
C1
C5
C14
C15
L4
L11
L157
L156
C34
TP1000
L154
C312
L155
L13
L15
R17
C27
C29
C33
C23 C24
C21
C25
C26
R15
TP24
R10
L10
C22
C28
L8
L9
IC3
C20
C19
CN4
L7
C18
R9
R11
R12
R13
R14
R16
C32
C35
C36
IC2
CN3
L6
C16
C17
1
C12
R8
C9
IC1
C13
L5
L2
C11
C10
L1
To CN xx Connectors board
CAB xxx
Q1
R2
JS4
JS5
JS3
JS1
JS2
1
To CN xx Power Supply board
CAB xxx
L12
C37
1
CN18
C43
C44
CN17
C38
L18
CN16
L17
CN6
IC6
C66
C68
C74
C76
L30
89 of 151
C77
L31
CN7
C75
J4
C67
C71
C73
Fig. 9.2.2 Layout and connections - Sheet 2
Version 3.0 – Revision 0 - 29/11/12
C61
C64
C63
L28
R25
C79
L29
C80
C56
C60
C72
C78
IC4
C58
C65
IC8
IC7
C70 L27
J3
To CN21 Power Supply board
CAB xxx
L24
L25
C57
C59
L26
C62
R21
R23
C69
R24
C48
L20
L23
C54
C55
IC5
L22
C53
L21
C52
R19
C51
R18
C50
C49
L19
C45
C42
C41
C40
C47
C39
C46
Chorus trio – SERVICE MANUAL
CHSMIT30
Internal BarCodeReader
CAB533
R22
R12
L38
C77 C78
R53
L26
C64
IC13
C57
C81
L36
CN20
To Instrument
Thermoregulation
Board CAB531
C84
L43
L37
C69
R91
R96
R77
R55
IC17
DL1
CN11
2 1
F1
CN12
IC19
R92
R84
R78
R72
C67
R64
C50
IC10
C68
CN17
PAD10
R46
R58
R56
CN10
C39
C62
L32
R100
R57
C49
R45
R26
PAD9
R35 R36
R17
C29
L31
C65
C55
C48
IC9
C66
C38
L3
C20
CN5
L23
L29
C58
C15
C80
C61
L2
X-Carriage
CAB513
L35
R16
R25
R54
PAD8
R33 R34
R15
C28
C14
L4
L42
C79
L39
C47
L1
C16
CN19
CN9
CAB515
C60
Optical Channel #5 ,#6
CAB516/3
C85
L34
CAB510
C37
CN22
CN18
R61
R59
R14
R24
R71
C27
PAD7
R31 R32
IC7
IC8
Tray
R76
C46
R13
Disp #2
Optical Channel #3 ,#4
CAB516/2
IC12
C36
C19
CN4
L17
C11
L18
C12
L19
C13
L20
C59
L21
C51 R63
R52
C76
L33
CAB510
R23
PAD6
R43 R44
R11
IC6
C26
CN21
C45
CN8
Disp #1
R83
R75
R70
C35
Tds CAB566
Optical Channel #1 ,#2
CAB516/1
R99
C63
L28
C18
L16
L30
C86
L44
C82
CN13
L40
CN14
C83
L41
To Chambe r Thermoregulation
Board CAB530
To Power Supply
Board CAB536
To Speaker
CAB543
Fig. 9.2.2 Layout and connections - Sheet 3
Version 3.0 – Revision 0 - 29/11/12
90 of 151
Sheet 3
Chorus trio – SERVICE MANUAL
CHSMIT30
IC11
R51
DL1
C125
BNX1
C132
BT1
C128
R60
CN9
IC10
R48
TP26
DZ3
X2
L49
L53
To Power Supply Board
CAB536
C147
DL2 DL3 DL4
C154
K1
C151
C156
R83
L57
L58
R187 DL6
CN14
C164
C163
R84
DL5
CN11
DZ4
91 of 151
R186
L138
To Display VGA
CAB xxx
C160
C159
L56
L55
TP15
L64
R184
R185
C290
C289
L139
C291
L140
C293
L141
C294
C295
L142
L69
L135
L63
R82
C165
R85
L71
C169
D4
L68
L66
L67
L70
C283
C284
TP16
C161
DZ5
IC50
R79
R78
R77
L143
C167
Fig. 9.2.2 Layout and connections - Sheet 4
Version 3.0 – Revision 0 - 29/11/12
C122
L54
TP14
C166
IC13
R59 C124
JP1
CN10
L65
D3
D2
D1
1
C102
R47
1
C292
C158
R81
L62
L61
L60
L59
To Touch Screen
CAB544
CN12
L43
C105
L44
C109
C112
L47
L48
TP22
C282 L42
C118
TP100
C162
L37
C94
L38
C98
C99
L40
C103
L41
IC17
IC19
FA3
C91
R41
C101
C281
C107
R45
C139
C142
C157
R71
C145
R73
R75
C153
L136
L134
L133
C108
L51
R67
R68
C146
R76
C155
R80
R56
DZ2
R46
L46 C111
R50
IC12
R53
C117
C113 TP27
C121
R57 R58
R55 IC15
R61
R62 R63
L50
R65
C131
C135
L52
R70
C149
R74
C152
IC18
R72
C150
L45
R66
C140
R69
C133
IC16
C137
C143
R44
DZ1
C130
C134
R54
R64
C138
C141
C120
C116
C119
C127
C126
C129
IC14
R52
C123
Sheet 4
IC49
C104
C115
L36
C89
C88
C87
R36
R35
R34
R33
R32
R31
C95
X1
L39
C97
C96
C92
R43 R183
TP10
C286
C285
L132
TP28
TP13
TP4
R49
C106
C110
C144
C148
C287
R40
TP101
C100
C136
C85
C288 L137
R42TP2
TP3
TP11
R39
C114
R38
C86
C93
R30
IC9
To Display QVGA
CAB xxx
MX1
C168
FA4
Chorus trio – SERVICE MANUAL
CHSMIT30
Sheet 1
Sheet 2
TP23
Q1
R2
JS5
J2
CN2
R7
R6
C4
C3
C2
L3
R5
R4
C1
C5
C23 C24
C21
L11
L157
L156
L15
C34
TP1000
L154
C312
L155
L13
C25
C27
C29
R17
R10
L10
C22
C26
R15
C33
TP24
L8
C19
L9
C28
IC3
CN3
IC2
C20
1
C12
C9
C14
C15
L4
L12
C37
1
C44
C43
R108
L18
CN18
CN17
C38
R104
R20
R107
IC11
R88
L27
C56
C8
R60 R68
R49
C11
C10
R5 R6
R97
C23
C17
CN2
C6
L11
C7
L12
C42
IC3
PAD3
R41 R42
C5
L2
L6
C16
C17
L7
C18
R9
R11
R12
R13
R14
R16
C32
C35
C36
CN4
IC15
L5
R102
C73
C74
C75
CN15
L22
C52
R8
R87
R81
R73
R67
R3 R4
CN6
PAD2
R39 R40
R48
C32
L10
R101
C70
C71
C72
R86
R80
R66
R62
C41
C7
C31
R19
IC1
C13
L8
C6
CN1
IC14
L1
C22
FA2
J1
R3
IC51
R47
R18
IC2
R1
CN1
1
R189
C4
L9
JS4
C40
JS3
C3
L24
C53
JS2
R1 R2
C2
L6
L7
JS1
C21
PAD1
R37 R38
L5
R93
R85
R79
R65
IC1
CN15
FA1
C30
C1
CN16
L17
CN9
R51
DL1
C125
BNX1
C132
BT1
C128
R60
IC17
JP1
1
C142
CN10
L53
C147
DL2 DL3 DL4
C154
K1
C151
C156
C160
C159
L56
L55
R83
L57
L58
R184
R185
C290
C289
L138
R187 DL6
CN14
C164
C163
L64
R84
TP15
DL5
CN11
R82
L63
R186
L135
DZ5
L139
C291
L140
C293
L141
C294
C295
L142
L70
C283
C284
TP16
C161
DZ4
C165
C169
R85
L71
C167
IC50
R79
R78
R77
L143
C292
L69
L68
92 of 151
CN7
R48
X2
L49
Fig. 9.2.3 Test Points and Settings (complete view)
Version 3.0 – Revision 0 - 29/11/12
CN6
IC11
C101
C281
C122
R59 C124
TP26
C139
L65
D4
C83
L41
IC13
L54
TP14
C166
L43
C105
L44
C109
C112
L47
L48
TP22
C102
R47
C118
L51
R81
C158
D3
C162
L37
C94
L38
C98
C99
L40
C103
L41
C282 L42
C108
DZ3
R56
DZ2
C157
R71
C145
R73
R75
C153
R41
IC10
L132
C107
R45
L45
C146
IC18
L62
1
C83
L32
IC49
R44
DZ1
R67
R68
R70
R74
CN12
C54
L36
C89
C88
C87
R36
R35
R34
R33
R32
R31
C86
L39
C97
C96
C133
C149
R72
L66
L67
FA3
C91
L133
C285
TP28
R46
L46 C111
R50
IC12
R53
C117
C113 TP27
C121
R57 R58
R55 IC15
R61
R62
R63
L50
R65
C131
C135
L52
C130
C134
IC16
R66
C140
D1
L40
IC4
L22
C53
L21
C52
R19
C51
R18
C50
C55
C119
R76
C155
R80
L35
C90
L136
L134
C286
R43 R183
C120
IC14
C116
R52
D2
C86
L44
C82
CN14
C287
IC19
L61
L60
CN11
F1
2 1
CN12
R25
C95
X1
C152
L59
L30
CN13
MX1
TP100
IC19
R92
R84
R78
DL1
C67
R64
R72
C68
CN17
C62
TP101
TP10
L32
R100
R46
R58
R56
C50
IC17
PAD10
R26
R57
C49
R45
R17
CN10
C39
IC10
C79
C93
C150
L37
C69
R91
R96
R77
C49
C45
IC8
L29
L36
CN20
C66
L29
C55
R69
R55
PAD9
R35 R36
C29
C137
C143
C144
C148
R27
L34
R38
C85
C288 L137
R54
R64
C138
C141
C65
C48
IC9
L31
L33
R28
TP4
R49
C127
C136
C81
R16
L23
C38
L3
C20
CN5
C15
C82
TP13
C115
C126
C129
C84
L43
C77
L31
L30
R40
C123
C80
C61
C58
R26
R42TP2
TP3
TP11
C106
C114
C74
C76
C75
J4
R30
R39
C67
C71
C73
C80
C81
C100
C85
R15
PAD8
R33 R34
R25
L2
L4
L35
CN9
L1
R54
C61
C64
IC6
C66
C68
L28
IC9
C110
L42
C79
L39
C47
C14
C16
CN19
C60
C37
C28
IC7
C77 C78
C64
IC13
C57
C56
C60
C63
C65
R37
C104
L38
L26
L34
IC8
CN22
CN18
R61
R59
R71
R14
PAD7
R31 R32
R13
C19
CN4
R24
C76
L33
R76
R53
L24
C58
C72
C78
R29
C92
R12
IC12
C46
C27
C70 L27
J3
C84
C59
L21
C36
IC7
R103
CN21
R83
R75
R70
R11
PAD6
R43 R44
CN8
L17
C11
L18
C12
L19
C13
L20
R23
R21
R23
C69
R24
C51 R63
R52
C48
L20
L23
L25
C57
C59
L26
C62
R99
C63
L28
R10
C45
C26
CN16
R51
C35
IC6
IC5
R95
R90
R74
R9
C18
CN3
R22
C87
L45
Q1
R69
PAD5
R29 R30
C25
R106
R8
IC16
C44
IC5
C54
R82
C34
C42
R50
L19
IC18
R105
R7
CN7
R21
C41
C40
C24
L25
PAD4
R27 R28
IC4
L13
C8
L14
C9
L15
C10
L16
R98
R94
R89
C43
C47
C46
C39
C33
C168
FA4
TP23
CHSMIT30
C30
C1
IC1
C21
R1 R2
C2
C3
PAD1
R37 R38
L5
L6
R93
R85
R79
R65
Chorus trio – SERVICE MANUAL
L24
C53
C40
R47
CN1
IC14
R18
L7
C4
L8
C31
R87
R81
R73
R67
R3 R4
CN6
R19
IC15
R60 R68
R49
R108
R107
R104
R88
L27
C56
IC11
R20
PAD3
R41 R42
C23
C17
CN2
C6
L11
C7
L12
C42
IC3
R5 R6
C5
R97
L9
R102
C73
C74
C75
CN15
L22
C52
C32
L10
R101
C70
C71
C72
R48
PAD2
R39 R40
C22
R86
R80
R66
R62
C41
IC2
C33
R50
R51
R99
C63
L28
C45
R12
R23
PAD6
R43 R44
R11
CN8
C59
L21
C51 R63
R52
IC12
L38
C77 C78
R53
L26
C64
IC13
C57
C81
L36
CN20
L37
C69
R91
CN11
2 1
F1
CN12
L30
C86
L44
C82
CN13
L40
CN14
C83
L41
Fig. 9.2.3 Test Points and Settings Sheet 1
93 of 151
IC19
R92
R84
R78
DL1
C67
R64
R72
C68
CN17
C62
L32
IC17
PAD10
R58
R56
R46
C50
R100
R57
C49
R45
R26
CN10
C39
IC10
Version 3.0 – Revision 0 - 29/11/12
C66
R77
R55
PAD9
R35 R36
R17
C29
C84
L43
C65
C55
C48
IC9
L31
R96
L3
C20
CN5
L23
C38
L29
C58
C15
C80
C61
L2
Sheet 1
L35
R16
R25
R54
PAD8
R33 R34
R15
C28
C14
L4
CN19
CN9
L1
C16
L42
C79
L39
C47
IC8
C85
L34
C60
C37
CN22
CN18
R61
R59
R14
R24
PAD7
R31 R32
R13
C27
R71
C46
IC7
C76
L33
R76
C36
C19
CN4
L17
C11
L18
C12
L19
C13
L20
C26
CN21
R83
R75
R70
C35
IC6
R103
R95
R90
R74
R10
PAD5
R29 R30
R9
C18
R22
C87
L45
Q1
R69
C44
C25
CN16
IC16
R106
R82
C34
IC5
IC18
C54
R105
R8
R21
L25
PAD4
R27 R28
C24
R7
CN7
CN3
L13
C8
L14
C9
L15
C10
L16
R98
R94
R89
C43
IC4
PROGRAMMAZIONE
MICROPROCESSORE J1, J2
SEGNALI DI PROGRAMMAZIONE
FPGA IC3, IC6
Chorus trio – SERVICE MANUAL
CHSMIT30
R1
CN15
FA1
CN1
FA2
J1
MASSA DIGITALE
Q1
R2
JS4
JS3
JS5
JS1
JS2
1
R189
J2
R3
CN2
IC51
R7
R6
C4
C3
C2
L3
R5
R4
C7
C8
C6
C1
C5
C14
C15
L4
IC2
C23 C24
L11
L157
L156
C34
TP1000
C25
C22
C21
L154
C312
L155
L15
C33
R17
R10
L10
C27
L13
R15
TP24
L8
C26
C29
CN4
IC3
C20
C19
L9
C28
CN3
L6
C16
C17
L7
C18
R9
R11
R12
R13
R14
R16
C32
C35
C36
1
C12
R8
C9
IC1
C13
L5
L2
C11
C10
L1
L12
C37
1
C44
C43
CN18
CN17
C38
L18
CN16
BATTERIA
L17
C58
C286
IC49
DL1
BNX1
BT1
C128
+12V
C125
C147
DL2 DL3 DL4
K1
MASSAANALOGICA
C151
C156
TP15
R186
R187 DL6
L58
C164
C163
R84
R83
L64
L138
94 of 151
L57
DL5
CN14
C160
C159
L56
L55
CN11
R82
R184
R185
C290
C289
L135
L63
DZ4
C165
R85
L71
C169
L139
C291
L140
C293
L141
C294
C295
L142
L69
L68
L66
L67
L70
C283
C284
TP16
C161
DZ5
IC50
R79
R77
R78
C167
Fig. 9.2.3 Test Points and Settings Sheet 2
Version 3.0 – Revision 0 - 29/11/12
R60
CN9
R51
C154
TP14
C166
C132
C139
D4
D1
1
C102
TP26
DZ3
L49
L51
L65
D3
D2
CN12
C122
X2
L54
L143
C292
C158
R81
L62
L61
L60
L59
FA3
IC13
R59 C124
TP100
C162
R48
R47
C118
+5V
IC17
L53
IC19
Sheet 2
L43
C105
L44
C109
C112
L47
L48
TP22
C282 L42
C108
JP1
CN10
C157
R71
C145
R73
R75
C153
R41
L37
C94
L38
C98
C99
L40
C103
L41
1
C142
C146
R76
C155
R80
R56
DZ2
R67
R68
R70
C149
R74
C152
IC18
R72
C150
C111
R46
L46
R50
IC12
R53
C113 TP27
C117
C121
R57 R58
R55 IC15
R61
R62
R63
L50
R65
C131
C135
L52
C130
C134
R66
C140
R69
C143
C133
IC16
C138
C141
R44
DZ1
L45
R54
R64
C137
C136
C120
C116
C119
C127
C126
C129
IC14
R52
C115
C123
C107
R45
R43 R183
TP10
L132
TP28
TP13
L133
C285
C101
C281
TP101
L136
L134
IC11
C287
IC10
L39
C97
C96
C95
X1
C104
R42TP2
TP3
TP11
TP4
R49
C91
C288 L137
R40
C106
L35
C90
L36
C89
C88
C87
R36
R35
R34
R33
R32
R31
C86
C93
MX1
LAMPADA
C83
R27
L34
R38
C85
CN7
C82
L33
R28
C100
-12V
C77
L31
L30
L32
R25
R26
IC9
C110
C144
C148
C74
C76
C75
J4
R30
R39
C114
C71
C73
R37
C92
BACK LIGHT DISPLAY
C67
C68
L28
C80
C81
C79
L29
C84
R29
IC6
C66
C65
ACCELEROMETRO
C61
C64
C63
C72
C78
C56
C60
IC8
IC7
C70 L27
J3
CN6
L24
L25
C57
C59
L26
C62
R21
R23
C69
R24
IC4
Test point canali analogici
TP2, TP3, TP4, TP10,
TP11, TP13
C54
C48
L20
L23
C55
IC5
L22
C53
L21
C52
R19
C51
R18
C50
L19
C49
C45
C42
C41
C40
C39
C47
C46
C168
FA4
Chorus trio – SERVICE MANUAL
CHSMIT30
9.2.2 DESCRIPTION OF THE TEST POINTS AND CPU 2010 BOARD JUMPERS
Test Point Number
Description
TP2
Positive of the tester on TP2 and negative on TP15. Measure of the analog signal of CH2. Signal variations from 0 to 2.5V
TP3
Positive of the tester on TP3 and negative on TP15. Measure of the analog signal of CH1. Signal variations from 0 to 2.5V
TP4
Positive of the tester on TP4 and negative on TP15. Measure of the analog signal of CH3. Signal variations from 0 to 2.5V
TP10
Positive of the tester on TP10 and negative on TP15. Measure of the analog signal of CH6. Signal variations from 0 to 2.5V
TP11
Positive of the tester on TP11 and negative on TP15. Measure of the analog signal of CH4. Signal variations from 0 to 2.5V
TP13
Positive of the tester on TP13 and negative on TP15. Measure of the analog signal of CH5. Signal variations from 0 to 2.5V
TP14
Positive of the tester on TP14 and negative on TP24. Check the presence of -12V. Also reported by the start-up of DL6.
TP15
Analogical Ground
TP16
Positive of the tester on TP16 and negative on TP24. Check the presence of +12V. Also reported by the start-up of DL5.
TP22
Positive of the tester on TP22 and negative on TP24. Check the presence of +5V. Also reported by the start-up of DL1.
TP24
Digital Ground
TP26
Positive of the tester on TP26 and negative on TP15. Measure of the analog signal of the lamp voltage. Signal variations from 0 to 2.5V
TP27
Positive of the tester on TP27 and negative on TP15. Measure of the analog signal of the accelerometer. Signal variations from 0 to 2.5V
TP28
Positive of the tester on TP26 and negative on TP15. Measure of the analog signal of the lamp voltage. Signal variations from 0 to 2.5V
JP1
[1-2] (Default). On/off control of the back light of the display. [2-3], lamp display always on.
J1
Programming condition for the microprocessor
J2
Reset condition for the microprocessor
J3
Reset condition for the microprocessor
J4
No Maskable Interrupt of the microprocessor
JS1
Closed (default). Programming signal FPGA IC3, IC6
JS2
Open (default). Programming signal FPGA IC3, IC6
JS3
Open (default). Programming signal FPGA IC3, IC6
JS4
Open (default). Programming signal FPGA IC3, IC6
JS5
Open (default). Programming signal FPGA IC3, IC6
Version 3.0 – Revision 0 - 29/11/12
95 of 151
Chorus trio – SERVICE MANUAL
CHSMIT30
9.2.3 DESCRIPTION OF THE TEST POINTS AND THE DRIVER 2010 BOARD JUMPERS
Jumper Number
Description
TP23
Positive of the tester on TP23 and negative on TP24. Check the presence of +24V. Also reported by the start-up of DL8.
Pad1
[2-3] (Default). Current setting for Wash1 motor. Minimum value
Pad2
[2-3] (Default). Current setting for Wash2 motor. Minimum value
Pad3
[2-3] (Default). Current setting for Wash3 motor. Minimum value
Pad4
[Open] (Default). Current setting for Syringe motor. Maximum value
Pad5
[1-2] (Default). Current setting for Filter motor. Mean value
Pad6
[2-3] (Default). Current setting for Tray Synchronization Device motor. Minimum value
Pad7
[2-3] (Default). Current setting for Disp1 motor. Minimum value
Pad8
[2-3] (Default). Current setting for Disp2 motor. Minimum value
Pad9
[Open] (Default). Current setting for Tray motor. Maximum value
Pad10
[Open] (Default). Current setting for Sliding motor. Maximum value
Version 3.0 – Revision 0 - 29/11/12
96 of 151
Chorus trio – SERVICE MANUAL
CHSMIT30
9.2.4 TROUBLESHOOTING
The CPU 2010-DRIVERS 2010 boards have been completely realized in Surface
Mount Technology (SMT), and therefore integrated circuits cannot be replaced
unless SMT reworking equipment is made available.
Version 3.0 – Revision 0 - 29/11/12
97 of 151
Chorus trio – SERVICE MANUAL
CHSMIT30
9.3 LOW POWER
9.3.1 DESCRIPTION
The Low Power board controls all the hydraulic actuators (solenoid valves,
electromagnets, pumps) and in which all the instrument’s functional sensors
(error sensors, tank sensors, alarm sensors) enter and are mixed.
The board is found on the right side, looking at the instrument from the rear, and
is fixed to the frame by 4 automatic hooks located in its 4 corners (see Fig.
9.3.1).
9.3.2 POWER SUPPLY AND CONNECTIONS
Fig. 9.3.2 shows all the connections for the board, which is powered directly from
the Power Supply Unit through connector CN4 and cable CAB 358.
The power supply voltages are +24V and +5V naturally at the ground (GND),
and their presence on the board is indicated through two LEDs ( DL1, +5V and
DL2, +24V).
There are also two fuses on the socket (F3, at 5V and F4 at +24V) to protect the
board and the instrument.
All these components can be seen in Fig. 9.3.3.
Fig. 9.3.1 Layout of the Low Power board in the instrument
Low Power Board
Version 3.0 – Revision 0 - 29/11/12
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Chorus trio – SERVICE MANUAL
CHSMIT30
To EV7
To EV1
To EV2
To EV3
To EV4
To EV5
Door Lock CAB 553
Short circuit
Strip Sensor
CAB 548
Tray Lock CAB 552
Door Lock Sensor
CAB 550
EV9 CAB 527
Short circuit
To CN5 CPU2003
Board
CAB 540
EV8 CAB 526
To Power
Supply Board
CAB 538
To CN4 - Level
Sensors Board
CAB 541
Fig. 9.3.2 Layout and connections - Sheet 1
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PP7 - CAB 524
PP4 - CAB 521
P1 - CAB 520
PP3 - CAB 519
PP1 - CAB 517
Needle2 CAB558A
PP6 - CAB 523
PP5 - CAB 522
Needle1 - CAB 558
Washing Well
#2 - CAB 529
PP2 - CAB 518
Washing Well
CAB 528
Waste
CAB 555
#1
Error
Waste
Warning
Level CAB 556
PP8 CAB 525
To EV12
Fig. 9.3.2 Layout and connections - Sheet 2 of 2
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Jumper Setting
JP1
1-2 (default)
TP1
Analogical
For solenoid valves
EV1,EV2,EV3,EV4 at 24V.
For EV at 12V pos. 2-3
TP2
Digital
ground
JP2
1-2 (default)
For solenoid valves
EV5,EV7 at 24V.
+24V LED
For EV at 12V pos. 2-3
+5V LED
F3 - +5V
Fuse
F4 - +24V
Fuse
Fig. 9.3.3 Test Points and Settings Sheet 1 of 2
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Needle 1 &
Activation LED
Fig. 9.3.3 Test Points and Settings Sheet 2 of 2
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9.3.3 TROUBLESHOOTING
All the integrated circuits (I.C.) mounted on the board are on the socket,
therefore they can be easily replaced in case of breakdown. The components that
are more susceptible to breakdown than the others are those controlling the
actuators (pumps, solenoid valves, solenoids).
Listed below are the actuators and the I.C. that control them.
Actuator
I.C.
Type
EV1, EV2, EV3, EV4
IC8
L293E
EV5, EV7
IC9
L293E
EV8, EV9
IC10
L293E
P1, PP1, PP2, PP3
IC11
L293E
PP4, PP5, PP6, PP7
IC12
L293E
PP8, EV12
IC13
L293E
Other possible board anomalies could be:
Anomaly
Countermeasures
LED DL1 is off
Replace fuse F3
(the +5V are not present)
LED DL2 is off
Replace fuse F4
(the +24V are not present)
Needle1 or 2 sensor does not work
(also LED DL3 does not turn on)
The level sensors do not work
Responsible I.C.:
IC1,IC2,IC3,IC4,IC5,IC6,IC7
Responsible I.C.: IC15,IC16,IC18
(various wells, tanks)
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9.4 POWER SUPPLY
The power supply unit is located in the lower left part of the Chorus trio (see Fig.
9.4.1)
and
can
be
accessed
from
the
rear.
Its main function is to transform the line voltage into low voltage direct current
needed to power the instrument.
Fig 9.4.1 Location of the power supply unit
Power supply
If the power supply unit needs to be repaired or replaced, follow the procedure
below.
1) Disconnect from the mains power supply.
2) Remove the protective housing
3) Disconnect all the connections described in Fig.4.3
4) Loosen the 4 screws ( F1-F4) Fig. 9.4.2, that fasten it to the frame.
5) Lift the rear part to allow it to come out of the profile of the plastic
housing and slip it out of the instrument.
If it needs to be opened, unscrew the 6 screws (V1-V6) that fasten the sheet
metal cover and then remove it by sliding it towards the rear part of the power
supply unit.
The boards contained in the power supply unit will be described in the following
chapters.
F2
Fig.
Power Supply Unit
9.4.2
V3
Chassis
V4
V2
V1
V5
V6
F3
F1
F4
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9.5 DESCRIPTION
The Rev.3 Power Supply board is responsible for different things inside the power
supply unit; from regulating the lamp voltage, to the controlling of the heater, to
the switching of the output voltages from the power supply unit and the signals
from the CPU 2010 that go to the Modem board, to powering the cooling fans.
Fig. 9.5.1 shows the positioning of the board in the case of the power supply
unit, while Fig. 9.5.2 shows the connections that go in and out of the power
supply unit.
Power Supply Board
Fig. 9.5.1 Location of the Power Supply Board
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A CN3 di
CHSMIT30
CPU 2010 CAB xxx
A CN6 di
CPU 2010 CAB xxx
A CN7 di
CAB xxx
PSU2
CN1
D1
CN2
CPU 2010
1
+5V
PP1
CN3
CN4
COM
IC1
+ V
Scheda Connectors
- V
Scheda Power Supply
IC6
N
CN2
L
MC
IC4
Q1
C3
C2
L2
L1
L
Q2
R5
R9
C4
Q3
R2
R3
C1
Q4
R4
R10
CN3
R1
R8
CN3A
N
Q2
- V
L5
L6
R6
DL2
R7
- V
PSU1
- V
+ V
+ V
+ V
CN5
C5
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TP1
JP1
L3
C6
L4
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CN1
CN9
L8
CN10
J1
CN8
DL1
C7
C8
L7
CN7
CN4
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CHSMIT30
To CN21 CPU 2010 V5 Board
CAB 535
To CN2 Modem BoardCAB 539
Power Supply PSU1
Fig. 9.5.2 Layout and connections - Sheet 1 of
2
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Lamp CAB 542
CHSMIT30
Heater CAB 554
CPU 2010 V5 Board
CAB 536-536A
Low Power Board - Printer
CAB 538-538A
Power Supply PSU2
Power Supply Fan
Fig. 9.5.2 Layout and connections - Sheet 2 of 2
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9.5.1 TROUBLESHOOTING
Listed below are possible problems that may be caused by the Power Supply
Board.
The lamp does not switch on
Is D2 on?
YES
check the connection that goes to the lamp or check the lamp
itself
NO
1) Are there 24V between TP1 (GND) and Pin 1 of CN2?
2) Are there 5V between TP1 and R1?
If both conditions are met this indicates that IC1 is damaged
and the board needs to be replaced
If 1) is yes and 2) is no, then the command is not arriving
from the CPU. Vice versa, if 2) is yes and 1) is no then the
power supply unit that regulates the 24V (PSU1) is damaged
and it needs to be replaced.
The heater doesn’t work
If you have an oscilloscope, place the probe between TP1 (GND) and TP3.
Do you see a square wave? YES
1) The heater is OK? (check the resistance continuity)
2) Are there 24V between TP1 (GND) and Pin 1 of CN2?
Replace the heater if 1) is not true or power supply unit PSU1 if 2) is not true.
change the board
NO
Try to replace IC5. If the defect continues,
To replace the board, disconnect all the wires and unscrew the 4 screws located
in its corners.
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9.6 CONNECTORS BOARD
CHSMIT30
9.6.1 DESCRIPTION
The function of the Connectors Board (C0nnectos 2010 V4) is to interface the
Chorus trio with the outside world. There are connectors to connect it to different
peripheral devices. Any faults to the board can jeopardize the possibility of
connecting the Chorus trio to an external PC, therefore making it impossible to
update the firmware or to simply communicate with the instrument.
Power supply
The Connectors Board is powered at +5V and at +3.3V between the CAB xxx
that originates from the CPU2010 Board. The presence of power can be checked
by observing whether LED Dl1 (+5V) and LED Dl2 (+3.3V) are on.
The board is fastened to the chassis with 5 screws located in the corners of the
board. If it must be replaced, just remove the connections, the fastons to the
power connector and unscrew the 5 screws. To make the extraction of the board
easier, the power socket must also be removed by loosening the two screws
holding it to the rear panel of the power supply unit.
Fig. 9.6.1 shows the layout of the board inside the chassis.
Fig. 9.6.1 Location of the Connectors2010 Board
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CN1
A CN6 CPU 2010 Board
CAB xxx
CN2
A CN3 CPU 2010 Board
CAB xxx
Fig. 9.6.2 Layout and connections Sheet 1 of 2
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C1
Q1
Q2
R3
R2
L1
C2
R1
L2
R4
C3
R8
Q4
R10
Q3
R5
R9
C4
R6
R7
DL1
DL2
CN3
TP1
C5
L4
C6
L3
C8
C7
L8
L7
L6
L5
CN3A
JP1
J1
CN4
CN5
Connettore
MEMORY CARD
Connettore
Connettore
Connettore
HOST
Fig. 9.6.2 Layout and connections - Sheet 2 of 2
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BARCODE
READER
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CHSMIT30
9.6.2 TROUBLESHOOTING
The board was realized using SMD technology, therefore the board cannot be
repaired in the field.
CN1
Q1
CN2
C1
Q2
R3
R2
L1
C2
R1
L2
R4
C3
R8
Q4
R10
Q3
R5
R9
C4
R6
R7
DL1
DL2
CN3
TP1
C5
L4
C6
L3
C8
C7
L8
L7
L6
L5
CN3A
JP1
J1
CN4
CN5
Fig.9.6.3 Signalling LED
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10 SERVICE PROCEDURES
10.1 PROGRAMMING CPU 2010
If the board ever needs to be replaced after a fault, use the following procedures
to speed up the technical intervention and to ensure a positive result.
Firstly, you must verify that you can connect to the instrument; this is done
through a serial cable from the "EXT SERVICE" connector to a PC which has the
WinChorus trio program.
10.1.1
SERVICE APPLICATION
Launch the program and check if the
instrument is connected by observing
whether the firmware release sign
appears. Then wait for a window to
open.
Fig. 10.1.1 Entry into the program
Fig. 10.1.2 Connection verification
Depending on whether we were able to connect with the instrument or not, we
have two choices:
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10.1.2
CONNECTION WITH CHORUS TRIO SUCCESSFUL
CHSMIT30
The first operation to perform is to download the instrument’s hardware
parameters onto our PC so that they can then be reloaded onto the new board.
To do this from the application click on Settings and then Parameters
Fig. 10.1.3 HW Parameters
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The hardware parameters setting window will appear. Click on Save to file
Fig. 10.1.4 HW Parameters
The name with which to save the parameters file is requested; give it a logical
name (i.e. Chorus trio followed by the machine’s serial number)
and then confirm with Save
Fig. 10.1.5 Saving the HW parameters
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10.1.3
SAVING METHODS
CHSMIT30
At this point we can start to save the methods, which are nothing more than the
tests the machine is able to perform.
From the application click on Methods and then on Save to file
Fig. 10.1.6 Transfer of Method Set
Fig. 10.1.7 Transfer of Method Set window
Now click on Save.
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10.1.4
CONNECTION WITH CHORUS TRIO UNSUCCESSFUL
CHSMIT30
If you were not able to connect, this means that something happened that relates to the
serial connection, and there are two possible scenarios:
1) The fault is hardware related; in this case you will not be able to connect in
any manner.
2) The microcontroller lost the information to be able to communicate with the
outside; in this case you can still connect by downloading a basic program
(loader.bin) into its memory which will allow us to dialogue with the board.
To verify which of the two situations we are in, do as follows:
Click on Commands from the main menu then on Firmware / Write
The window instructing you to press the programming button will appear.
Fig. 10.1.8 Initial programming
Select the programming file and click on Open.
If we get a Device error message, this means that hardware is the problem and
you can try to install the loader program. If this is not possible, the only other
option is to change the board.
Fig. 10.1.10 Time-out Error
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If the connection is active the Chorus
By clicking on Write the following window will appear and the *.bin file will be
downloaded into the micro-controller’s memory
Fig. 10.1.11 Check Sum
But if a communication error appears, as in this case, a loader program must be
installed.
To do this, with Chorus manager active, insert the Boot loader board at the USB
cable and wait for the Message Box:
Insert the Boot loader onto the CPU2010 board and wait.
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Then switch off Chorus and disconnect the Boot loader board, then reprogram
the Chorus in the previously indicated manner.
We now need to reload the previously saved parameters and methods.
10.1.5
LOADING OF PARAMETERS AND METHODS
Turn on the instrument. The errors regarding the hardware parameters will
appear on the first screen.
From the main menu of Chorus manager trio (on the PC) click on Settings and
then Parameters
Fig. 10.1.14 Loading of HW Parameters
The hardware parameters setting window will appear. Click on Load from file
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Fig. 10.1.15 HW Parameters Window
The name of the file to load will be requested. Select the file that we previously
saved and then confirm with Open
The hardware parameters setting screen will again appear. Click on Store.
Lets now move to the loading of the methods.
From the main menu of WinChorus trio (on the PC) click on Methods and then on
Load from file
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Now click on Open and the previously selected parameter will be saved.
Load all the methods by clicking on Store.
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10.2 PROGRAMMING THE TC1100
10.2.1
PREREQUISITES
The following must be available in order to program the TC1100 barcode reader:
•
“sm@rtset rel 1.10” configuration software from Datalogic (setup is
found in the “*” folder of the CD)
•
“serial interface for TC110.xml” configuration file, located in the “*”
folder of the CD
•
Premade serial connection cable (see diagram)
•
A 5V power supply unit to power the reader
•
A PC with an open serial port
10.2.2
CONNECTING THE READER TO THE PC
Connect the RS232 connector of the premade cable to an open serial port on the
PC and connect the two molex connectors in their respective sockets (J1 and J2)
on the reader.
Connect the 5V power supply unit to the cable and check that the red LED on the
reader turns on.
10.2.3
INSTALLATION OF THE CONFIGURATION SOFTWARE
Launch the setup file sm@rtset rel 1.10.exe and follow the instructions.
A new “Datalogic” link will be created in the programs section in the Start Menu.
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10.2.4
LAUNCH THE CONFIGURATION PROGRAM
CHSMIT30
Launch
the
program
Sm@rtset
(start
menu/programs/Datalogic/sm@rset/sm@rtset) and close the information screen
Select Load from file in the window below
Select the “serial interface for TC110.xml” configuration file (can be found on
the CD)
A summary screen will be displayed; click on end
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Select the interface entry from the right menu and check the reader parameters
(in the example window, the reader is already set in RS232 mode).
Click on the synchronize icon (circled in red)
In the window below, click the button circled in red; a notice will inform you that
the device was recognized. Click on exit
Check that the Save in reader’s EEPROM option has been selected and click on
Write configuration
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Click on next
The configuration summary screen
Click on end and exit the program.
will
be
displayed
when
The device has now been correctly programmed.
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10.2.5
CHSMIT30
WIRING DIAGRAM OF THE CONNECTION CABLE
The power must be supplied externally through a 5V power supply unit.
-
J2
Jack
1
2
3
4
5
6
7
8
+
Molex 51021-0800
6
1
J1
1
2
3
4
5
6
7
8
Gnd
Rx
Tx
Molex 51021-0800
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10.3 PROGRAMMING THE DLC6065 BARCODE READER
The CCD DLC6065 reads barcodes automatically as well as on contact. The front
window projects a line of light which must cross the entire code.
The best conditions for reading are obtained when the reader handle is kept
parallel to the surface on which the code is found (see fig. 10.4.1).
Fig. 10.4.1.
10.3.1
CONNECT THE READER TO THE INSTRUMENT THROUGH THE RS232 CABLE
Insert the RJ connector of the RS232 cable (CAB350) into the socket located at
the base of the reader and push until the tab clicks into place; connect the other
end of the cable to the serial port on the Chorus trio labelled Barcode (fig.
10.4.2).
Warning: since the serial port also supplies the power, it is best to connect the reader with the
instrument off.
Fig. 10.4.2.
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10.3.2
DISCONNECT THE RS232 CABLE
CHSMIT30
To disconnect the cable from the reader, just lightly push a pointed object (i.e. a
paper clip) into the hole located near the base and slip off the RJ connector (fig.
10.4.3).
Fig. 10.4.3.
10.3.3
ENABLING SERIAL COMMUNICATION
The DLC 6065 reader exits from the factory ready for use with the Chorus trio.
If, for any reason, the reader loses the default configuration, serial
communication must be restored. This operation is done by having the reader
scan the codes reported below in sequential order (to read a code just rest the
wand on the code and press the button).
•
The reader goes into configuration mode by scanning the first barcode
(command $+)
•
The reader enables the serial interface by scanning the second barcode
(command CP0)
•
The reader saves and exits the configuration mode by scanning the third
barcode (command $-)
Note: This operation can be done from any software window.
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10.4 PROGRAMMING BARCODE READER ZEBEX Z 3080
The CCD Z 3080 reads barcodes automatically as well as on contact. The front
window projects a line of light which must cross the entire code. The best
conditions for reading are obtained when the reader handle is kept parallel to the
surface on which the code is found (see fig. 10.5.1).
Fig. 10.5.1.
10.4.1
CONNECT THE READER TO THE INSTRUMENT THROUGH THE RS232 CABLE
Insert the RJ connector of the RS232 cable (CAB350) into the socket located at
the base of the reader and push until the tab clicks into place; connect the other
end of the cable to the serial port on the Chorus trio labelled Barcode (fig.
10.5.2).
Warning: since the serial port also supplies the power, it is best to connect the reader with the
instrument off.
Fig. 10.5.2.
10.4.2
DISCONNECT THE RS232 CABLE
To disconnect the cable from the reader, just lightly push a pointed object (i.e. a
paper clip) into the hole located near the base and slip off the RJ connector (fig.
10.5.3).
Fig. 10.5.3
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10.4.3 PROGRAMMING
PARAMETERS
This table gives the default settings of all the
programmable parameters.
The default
settings will be restored whenever the
"Reset" programming label is scanned and
the laser scanner is in programming mode.
DEFAULT VALUES
PARAMETERS
OF
OPERATING
Function
Default
Values
Scanning Mode Selection
Trigger mode
Header and trailer
None
Inter-Message delay
Normal
Inter-Character delay
Normal
Message/Block mode
selection
Message
Send command in block
mode
Code identifier
transmitting
Disable
PREDEFINED
IDENTIFIERS*
MSI barcode identifier
code
P
MATRIX 25 barcode
identifier code
G
DEFAULT VALUES OF KEYBOARD
EMULATION PARAMETERS SETTING
Function
Keyboard
selection
Default Values
type
IBM PC/AT
USA
Message terminator
Enter/ carriage
Return
DEFAULT
VALUES
OF
RS-232C
SERIAL
COMMUNICATION
PARAMETERS
Function
Communication
Medium
L
*
Disable
Good read beeper tone
selection
Code 93 barcode identifier
code
BARCODE
Default Values
Handshaking protocol
None
ACK/NAK response
time setting
300 msec
Baud rate
9600
Data bit
8
Stop bit
1
Parity
Mark
Message terminator
selection
CR/LF
Code 39 barcode identifier
code
M
DEFAULT
VALUES
OF
EMULATION PARAMETERS
ITF 2 of 5 barcode
identifier code
I
Function
Chinese post code
identifier code
H
*
*
WAND
Default
Values
Wand emulation speed
Normal
Wand emulation output
Black
High
=
UPC-E barcode identifier
code
E
UPC-A barcode identifier
code
A
Note: For wand emulation, the configuration
is only effective for the items with asterisk
(*).
EAN-13 barcode identifier
code
F
EAN-8 barcode identifier
code
DEFAULT
VALUES
OF
EMULATION PARAMETERS
FF
Codabar barcode identifier
code
N
*
Code 128 barcode
identifier code
K
*
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Function
Default
Values
Keyboard Type
US
Keyboard
Message Terminator
Enter
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DEFAULT VALUES
PARAMETERS
Function
Reading
codes
*
Selection
*
OF
Code
Default
Value
Code 39
Enable
ITF 2 of 5
Enable
Chinese Post
Code
Disable
UPC/EAN/JAN
Enable
Coda bar
Enable
MSI
Enable
Code 93
Enable
EAN-128
Disable
*
MATRIX 25
Disable
Disable
Codes
Standard
Start/stop
characters
Not
transmitting
Check digit
Disabled
Concatenation
Off
Interleaved
Length
6-32 digits
2 of 5
Check digit
Disable
Chinese Post
Length
10~16 digits
Code
Check digit
Transmit
Format
All
Addendum
Disable
UPC-E=UPC-A
Disabled
UPC/EAN/JAN UPC-A leading
digit
Transmit
Coda bar
Code 128
UPC-A check
digit
Transmit
UPC-E leading
digit
Transmit
UPC-E check digit
Transmit
Type
Standard
Start/stop
characters
A, B, C, D
Length
6~32 digits
FNC 2 append
Disable
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Length
Variable
Check digit
Transmit
Italian
Pharmacy
Transmit "A"
Character
Not
transmitting
MATRIX 25
Length
Fix 10 digits
Check digit
Disable
MSI
Note: The configuration of the items with
asterisk
(*) is effective when being appointed in advance.
PROGRAM
PROCEDURE
BARCODE MENUS
Disable
ISSN/ ISBN
Code 39
Disable
Disable
*
*Italian
Pharmacy
Check digit
Disable
Code 128
ITAT
CHSMIT30
DECODING
NO
NO
YES
YES
SYSTEM SETTING
Start of Configuration
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

RESET
CHSMIT30
The reading of the
"RESET” label turns
all the parameters
back
to
default
values.
When you intend to
turn your scanner
back
to
default
parameter,
please
scans the "Start of
configuration"
label
first,
then
scan
"RESET" label
• The
reading
of
"ABORT"
ABORT
all
parameters
Reset
PC/AT
End
2. Quick Settings for RS 232 Mode
the
label
discards
Program
the
Program
Reset
RS-232C
End
read
prior to the "End of
configuration".
RS-232C
• The scanner remains
in the last interface
mode
PC/AT
when
the
scanner is reset. The
label below should be
scanned
if
Program
German Keyboard
the
scanner is configured
USB
the first time.

WAND EMULATION
3. Quick Settings for German Language Keyboard
The reading of the
“SHOW
VERSION”
label will be show
firmware version.
End
SHOW VERSION
End of Configuration
QUICK SETTING
1. Quick Settings for Keyboard Wedge Mode
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10.5 MAINTENANCE
10.5.1
ROUTINE MAINTENANCE
Must be done as needed by the user and involves the replacing of consumables,
which are:
• The replacement of the wash and buffer tanks when indicated by the
instrument
•
The replacement or emptying of the waste tank.
•
The thermal paper for the printer.
If an initial check is performed at instrument start-up, a control procedure of
every device (electronic, mechanical and hydraulic) is carried out.
This procedure involves:
•
priming if the instrument was idle for more than 5 hours
•
washing of the internal parts
•
checking the functionality of the syringes, washers and dispensers
•
check of the optical calibration.
Any anomalies or errors will appear in the error window and, depending on the
type, may or may not be removed by the user (see Operating Manual).
The wash procedure must be launched at the end of the work day in order to
prevent incrustations from forming on the needles, tubes, etc. (see Operating
Manual).
The sanitization procedure is one of the maintenance operations to be
performed at the user’s discretion.
This procedure is similar to the washing except that a tank of antibacterial
solution
is
used
in
place
of
the
cleaning
solution.
The purpose of this procedure is to eliminate bacterial colonies and fungi
which, over time, can begin to grow in the hydraulic circuit.
This procedure can even be performed at a frequency of every two weeks if
more than 30,000 tests are performed on the instrument per year.
Refer to the operating manual for the performing of this procedure.
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10.5.2
PERIODIC MAINTENANCE
CHSMIT30
Must be performed by Technical Personnel and involves working on the inside
of the instrument. The material needed to perform the maintenance, besides the
standard supplied replacement parts, are the following:
1. Sponges for the dispenser needles
2. Springs for the dispenser needles
3. Springs for the washer needles
4. Strip holder springs
5. Dispenser needle
6. Aspiration needle
7. Washer needle
8. Sleeve DBL 408 or LBBR4
9. Novoprene tube 2.5 x 1 for Sr 10/30
10.Novoprene tube 4.1 x 1.6 for Sr 10/50
11.Tuva tube 1.5 x 3
12.Cristal tube 3 x 5
13.Cristal tube 6 x 9
14.Cristal tube 9 x 13
15.Diaphragm for pump NF 30
16.Hydraulic fittings:
• CPC MS 2
• CPC ME 2
• Standard WES 6 1/8
• Standard GES 6 1/8
• Standard WES 8 1/4
• Value X230-6
• Value Y220-6
• Value Y210-6
• Value T210-6
• Value AC-6
17.OR 2-015
18.OR 2-004
19.OR 2-007
20.OR internal diameter 8 mm thickness 1mm
21.Optical filter 650
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22.Optical filter 610
CHSMIT30
23.Heat filter 03FHA007
24.Toothed belt T 2.5 x 305 H 20
25.Toothed belt T 2.5 x 480 H 6
26.Probe
27.Sensor screws
28.Miscellaneous screws
29.Gloves
30.Safety glasses
31.Small jars (20 x 20 x 15) for immersing the parts
32.Cleaning liquid with disinfecting and degreasing properties
33.WD40 (lubricant spray)
34.Silicone oil
35.Silicone grease
36.Needle cleaning pads (0.6 0.8 mm drill bit)
37.Pad for cleaning the probes (steel or PVC rod, diameter 2.5 mm length 30
cm)
Use the following precautions before starting any intervention:
-
Check the number of tests performed since the last maintenance intervention
-
Start the sanitization procedure
-
Unplug the instrument from the electrical power outlet
-
Wear protective glasses and gloves
The maintenance for each device will be described in the following paragraphs.
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10.5.3
WASH WELL FOR THE DISPENSER NEEDLES
CHSMIT30
Dirt accumulates in the flat area around the slit from which the well is
accessed, above and inside the well itself (see Errore. L'origine riferimento
non è stata trovata.0.5.1 )
The following is therefore required:
•
Disassemble the wash well
•
Remove and replace the sponges with new ones
• Disassemble all the hydraulic fittings, including the sprayers, removing all
possible gaskets or sealing elements
• Immerse all the parts in good condition in a disinfecting and cleanser
solution and replace any worn parts.
Sponge #2
Fig. 10.6.1
Sponge #1
Dry and reassemble all the parts of the well, making sure that the teflon gaskets
have been replaced. Before remounting it on the platform, clean it.
Fig. 10.6.2
Hydraulic
wash
fitting
Hydraulic
drain
fitting
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10.5.4
DISPENSERS #1 AND #2
CHSMIT30
Dirt mainly accumulates: on the needles, at the base of the dispensers, on the
drive and around the slider guide.
The following is therefore required:
-
Disassemble the two dispensers from the X carriage
-
Disassemble the needles
-
Check that the needles are in good condition and that the line is perfectly
open and free of incrustations. If the user complains about perforation errors,
replace the suspected needle and replace both after 5000 tests.
-
Check the needle-cable sensor contact.
-
Disassemble the bracket guide, clean any incrustations with WD40 and
lubricate the guide with silicone oil.
-
Check the needle spring and replace it if rusted.
-
Reassemble the unit without mounting it onto the drive.
Fig. 10.6.4 Dispenser
Bracket
Guide
Needle
Fig. 10.6.3 Dispensers #1 and #2 mounted on the X carriage
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10.5.5
CHSMIT30
X-AXIS GUIDE
-
Loosen the screws holding the motor in order to loosen the belt
-
Remove the drive from the guide carriage and clean it
-
Disassemble the plate guide, being careful that the carriage does not come
off, then clean and lubricate it.
-
Check that the belt is not worn, otherwise replace it.
-
Clean the platform before remounting everything.
Belt
Platfor
Drive
Motor
Guide
Fig 10.6.5
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10.5.6
CHSMIT30
WASHERS 21 – 25 – 28
Dirt mainly accumulates on the needles and at the base of the washers
(see Fig. 10.5.6)
-
Remove the three washers from the plate
-
Remove the needles from the washers
-
Check that the needles are in good condition and that the lines are perfectly
open and free of incrustations.
-
Remove the guide from the bracket for all washers
-
Clean the bracket and the guide and any incrustations with WD40 and
lubricate the guide with silicone oil.
-
Check the needle springs and replace them if rusted or after 5000 tests.
-
Check the spherical slide couplings of the needles. Replace if worn or
encrusted.
After the platform has been cleaned, the units can be remounted.
Bracket
Guide
Fig 10.6.6 Washer dirtiness
Needl
Fig. 10.6.7 Washers #1 and #2
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10.5.7
CHSMIT30
OPTICAL UNIT
During normal operation dirt and dust deposit on the tray and on the lenses of
the optical unit, which must therefore be removed and cleaned separately.
The optical unit is made up of two parts:
•
Emitter: a plastic block on which the 2 optical fibers and the lens plate are
mounted. This piece is mounted on the upper platform
•
Receiver: a plastic block on which the printed circuit for the optical
receivers and the lens plate are mounted. This piece is mounted below the
lower platform
Let’s see where they are positioned in the machine.
Layout of the emitters
Emitter 3-4
Emitter 1-2
Emitter 5-6
Optical fibres
Fig. 10.6.8
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To remove the emitter from the machine, just loosen the two screws fastening it
to the upper platform. When lifting it from the platform, we can now see from
below the lenses that we will take out by removing the two screws.
Using a cloth, delicately clean the lenses, being careful not to scratch them.
Warning: when the lenses are remounted, their convexity direction must be
respected.
Light emitter
Fastening
screws
li ht
itt
Lenses
Lens fastening
screws
Fig. 10.6.9
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Positioning of the Receivers
As mentioned previously, the receivers are mounted below the lower platform.
The figure below shows their layout
Receiver 5-6
Receiver 5-6
Receiver 1-2
Fig. 10.6.10
To remove the receiver, loosen the two screws fastening it to the lower platform.
Slide it from its seat and unplug the wire from the board. Now that we can see
the lenses, take off the plastic by removing the two screws.
Using a cloth, delicately clean the lenses, being careful not to scratch them.
Warning: when the lenses are remounted, their convexity direction must be
respected.
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Receiver
Receiver
board
Lenses
Lens fastening
screws
Fastening
screws
i
Fig. 10.6.11
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10.5.8
CHSMIT30
WASHER WELLS
 Remove the two wells from the lower platform by loosening the screws,
 Clean the sensor contacts, removing any incrustations on the contacts and
in the body of the well
 Check the conductivity of the sensors
 Make sure that the platform is clean before repositioning them.
Sensors
Well
Fig. 10.6.11
10.5.9
UPPER PLATE
Dirt accumulates in the points where the devices are mounted, therefore clean
the platform around the devices, especially in the slits and any other places
where dirt is found. Use disinfecting/cleaning liquid and dry.
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10.5.10 CAROUSEL
The cleaning of the carousel is done in two phases:
-
upper plate of the tray, accessing the slit on the platform in
correspondence with the x-axis shifting (see Fig. 10.5.12) and clean,
sector by sector, both the upper part and inside the strip positioning
chamber.
Be sure not to use abrasive liquids and/or substances that can remove the numbering printed on the
tray.
Fig 10.6.12
Check of the strip holding springs.
Insert a strip in each of the thirty strip positions and check that the spring holds
it in place. Replace it if it does not hold.
To remove the springs, take off the sealing ring to which the fastening screws
access from the special opening in the platform.
10.5.11 PERISTALTIC PUMPS
•
Disassemble the heads, leaving the pump in its place
• Remove and clean the plastic residues on the rollers and on the internal
walls of the head
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•
Replace the tube
•
Remount the head
CHSMIT30
Fig 10.6.14
10.5.12 DIAPHRAGM PUMP
•
Disassemble the head of the pump
•
Clean the internal diaphragm and the head
Remount the head, being careful of its direction of flow in order to avoid
switching the aspiration/expulsion flow
(see fig.10.5.7)
Fig 10.6.15
10.5.13 HYDRAULIC WASTE AND SYRINGE UNIT
Dirt mainly accumulates in the contact points between removable parts (ex:
covers, hydraulic fittings, sensors, caps, brackets, etc. (see fig. 10.5.9). Perform
the following steps for a deep cleaning, using the normal disinfecting/cleaning
liquid.
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• Disassemble the waste well unit by removing the two screws holding it to
the instrument’s frame.
•
Remove the syringe unit (Fig. 10.5.9) from the bracket (1).
Fig 10.6.16
10.5.14 HYDRAULIC UNIT
-
Disassemble the head of the peristaltic pump (PP8)
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-
Remove and clean the plastic residues on the rollers and on the internal walls
of the head
-
Replace the tube and remount the head.
-
Remove the waste well (from the rod) and completely disassemble it.
-
Clean them by immersing in cleaning liquid for 30’.
-
Clean the sensor contacts.
-
Check all the hydraulic fittings and replace those that are worn.
-
Reassemble the wells, remount the fittings and replace the seals (teflon and
silicone).
-
Check the conductivity of the sensors
-
Check the cleanliness of the tubes and replace them if they are not clean.
-
Reposition the well on the rod (2)(see fig.10.5.8)
Sensor
Fig. 10.6.17
10.5.15 SYRINGE UNIT
-
Unscrew the syringes from the Plexiglas support
-
Extract the piston from the syringe, clean it and grease the teflon tip with
silicone
grease
(this
operation
requires
a
lot
of
skill)
Warning: when handling the syringe, do not push the piston so that it exits the side of the ring nut
otherwise the tip will become damaged when it is reinserted.
-
Check the conditions of the syringe O-rings and replace them if necessary.
-
Screw the syringe onto the Plexiglas support and fasten the piece.
-
Check the hydraulic fittings by disassembling them from the Plexiglas
manifold, removing the silicone
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-
Put new silicone on the fittings and reposition them on the Plexiglas manifold
-
Clean the syringe feed screw with WD40 and lubricate with silicone oil.
-
Check the tightness of the screws, especially those for the joint
-
Reposition the syringe on the cross beam (1 Fig. 10.5.8 ) and remount
everything on the instrument’s frame.
250
μl
Plexiglas
support
Fig 10.6.18
10.5.16 ROTATION
The rotation requires no special maintenance operations besides cleaning of the
dust and checking the voltage and the condition of the belt.
10.5.17 TRAY SYNCHRONIZATION DEVICE
Check the tightness of the screws of the coupling for the tray synchronization
device
10.5.18 LAMP BOX AND FILTER HANDLER
-
Remove the lamp box from the LCD support
-
Remove the filter handler from the lamp box
-
Remove the support guide, clean it and lubricate it
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-
Remove and clean the filters, being careful not to scratch them or leave
halos. Check their overall condition and replace if necessary
-
Check the OR spacer for the filters: when the filters are mounted, their
container must have no play.
-
Check the heat filter. Clean and replace if broken or worn.
-
Remove any trace of residue and dust.
-
Remount the filter handler
-
Remount the lamp box
-
Remount the box onto the LCD support
10.5.19 TANK PROBES
Dirt mainly accumulates in the hydraulic piping (incrustations) and on the sensor
contacts (incrustations and rust).
For incrustations on the sensors, clean them with disinfecting/cleaning liquid.
In case of rust, clean with WD40 and disinfecting/cleaning liquid and/or replace
the probe.
Check the conductivity of the sensors
Check the hydraulic piping.
10.5.20 TUBING
This check pertains to all the tubes in the instrument. Replace any tube that has
mould or other residues.
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