Download Biosum User Manual

Sidena 24/06/2007
User Manual
Ver 1.2
_____________________________________________________________________________________________________
BIOSUM
DIFFERENTIAL CELL COUNTER
USER MANUAL
SIDENA
302, avenue de Neuville 78950 Gambais
[email protected] 01 34 87 19 05 01 34 87 19 06
JPD 17/07/07
Sidena 24/06/2007
User Manual
Ver 1.2
_____________________________________________________________________________________________________
Overview
Biosum is a stand-alone differential cell counter which facilitates observation, counting and
classification of cells into 24 different categories.
It can store results in its own memory, transfer them into a PC and/or print directly to a serial printer.
The PC software provided with Biosum can transfer the results to an Excel sheet, and it gives the
possibility to rename the names and mnemonics of all the keys for specific uses.
Operating modes
Biosum is particularly designed for the characterization of spermatozoa, and it shows the results
according to the David method or according to the Kruger method.
It can also be useful for hematology, or generally for any application requiring the classification of cells
or tissues.
Kruger Method
Professor Kruger's method takes into account only the main anomaly of a spermatozoon.
At the end of the counting, Biosum displays the percentage of typical forms.
David Method
The method described by Professor David counts the spermatozoa by noting up to six
abnormalities per specimen, and calculates the IAM (multiple anomalies index). The screen
cells display the number of times when the abnormality has been noted and the number of
times it was associated with one or more other abnormalities.
Hematology mode
The absolute values can be displayed after entering the number of white cells.
The keyboard
The keyboard comprises 30 keys divided into 6 function keys (the 6 keys closest to the screen) and 24
counting keys divided into 4 lines of 6 keys. The key # 1 is placed bottom left side, key # 6 is bottom
right, and so on until the 24th key, which is placed top right side.
Generally, key # 25 is the ESCAPE key and key # 30 is the validation key (ENTER).
SIDENA
25
26
27
28
29
30
19
13
7
1
20
14
8
2
21
15
9
3
22
16
10
4
23
17
11
5
24
18
12
6
302, avenue de Neuville 78950 Gambais
[email protected] 01 34 87 19 05 01 34 87 19 06
JPD 17/07/07
Sidena 24/06/2007
User Manual
Ver 1.2
_____________________________________________________________________________________________________
Getting started
The AC adapter is suitable for supplies from 100 to 260 VAC. Plug in the adapter and switch the
Biosum on with the switch placed at the rear of the case.
It displays the following screen, which is the Main Menu :
QUIT
LIRE
CONF
XFER
EFFAC
COMPT
BIOSUM - V2.2
Hematologie
2 formules en memoire
29/09/06
10 :28 :17
Choisir une touche fonction
The upper line shows the functions available with the 6 keys functions. The actions will change
according to the situation. Here we have the following set of choices :
QUIT
READ
SETUP
XFER
ERASE
COUNT
to leave,
to read the formulas recorded in memory
to configure Biosum
to connect to a PC
to ERASE all the formulas recorded in memory
to start a new counting
Formulas in memory can be erased one by one inside the READ function, or completely by the
ERASE function.
Counting
One must initially enter the identification of the sample. It can have up to 8 characters including figures
0 to 9 and slash (/). The function key “DEL” erases the last character ; function key “ESC” goes back
to the main menu. The “OK” function key validate the identification and starts counting.
The total number of cells (typical and atypical) is displayed at bottom right of the screen, followed by
the character “/” and by the target number to be reached. When starting the counting, the target
number is equal to 100. When one reaches with this number, Biosum sounds and gives a warning. It
is possible then to confirm the end of the counting, or to add one or more times 50 to the target
number by striking the key “+50”. Counting can then be continued.
Thus, if one decides to count until 200 cells, one can press 2 times on the key “+50” before starting
counting.
It is possible to stop counting at any time by striking then “END” function key. Then the counting can
be validated, continued, or aborted.
The “DEL” key cancels the last pressed key ; key “%” displays the results as percentages.
SIDENA
302, avenue de Neuville 78950 Gambais
[email protected] 01 34 87 19 05 01 34 87 19 06
JPD 17/07/07
Sidena 24/06/2007
User Manual
Ver 1.2
_____________________________________________________________________________________________________
The KRUGER method :
- each key increments the content of the corresponding grid cell on the screen.
- the normal spermatozoa must be counted with key # 18 “TYPICAL”.
- key # 6 as no effect.
- striking keys # 19 to 24 (upper line) do not increase the cumulated total counting. These keys are
used to record elements outside the main counting (isolated flagella, for example).
- the line at the bottom of the screen shows the number of typical spermatozoa, the number of atypical
spermatozoa, and the percentage of typical.
The DAVID method :
- the normal elements must be counted directly by sriking on key #18 “TYPICAL”. This increases the
content of the corresponding grid cell.
- the abnormalities are noted by striking one or more abnormality keys ; an asterisk character appears
in the corresponding screen cell. One can note up to 6 abnomalities on one spermatozoon.
- the abnormalities are then validated by the key # 6 “ENTER”. This increments the first figure in the
abnormalities cells and erases the asterisks. The second figure of each cell indicates the number of
times where this abnormality was associated with one or more other abnormalities.
- keys # 19 to 24 (top line) do not increase the total counting. The corresponding cells are immediately
incremented by each striking ; these keys are used to record elements outside main counting (isolated
flagella, for example).
The bottom of the screen displays the index of multiple anomalies (IAM).
Hematology counting :
Striking any key increments the contents of the corresponding screen cell.
The normal (or typical) cells must be counted by striking key # 18 “TYPICAL”.
Key # 6 has no effect.
Function key "ABSO" requires to enter the number of white blood cells, and then displays the absolute
values.
Keys # 23 and 24 do not increase the total counting. These keys are just used to record
characteristics elements which must not be taken inside the cumulated number of cells. The last key
inside then main count is thus key # 22, but it can be changed by the PC software. Its value must be
between 19 and 24 (in the upper key line).
SIDENA
302, avenue de Neuville 78950 Gambais
[email protected] 01 34 87 19 05 01 34 87 19 06
JPD 17/07/07
Sidena 24/06/2007
User Manual
Ver 1.2
_____________________________________________________________________________________________________
The other menus
ESC
MODE
BEEP
CSTD
DATE
IMPR
SETUP MENU
MODE : KRUGER
Beep : YES
Printer : NO
Strike a function key
SETUP menu
•
•
•
•
•
•
•
ESC sends back to the main menu
MODE selects the operating mode :
o Hematology counting
o Spermatozoa counting following DAVID method
o Spermatozoa counting following KRUGER method
BEEP sounds when striking a key
CSTD resets the standard key names (one can change cells names and
mnemonics with the PC software).
DATE sets date and time (it can also be done by the PC software)
IMPR selects the type of serial printer connected to Biosum if any.
Setting date and time
EXIT
DEL
OK
1
2
3
4
5
6
9
8
9
0
/
Entrez date format Jj/Mm/An puis OK
Date :
Enter the date following the format indicated obave, including character "/".
For entering time, the separator character is ": ".
In case of bad keying, the clock will not start.
Date and time are hold by a small internal battery during about fifteen days.
If Biosum is left unused during a longer period, it can be necessary to reset date and time.
Date and time can also be set very easily by the PC software.
SIDENA
302, avenue de Neuville 78950 Gambais
[email protected] 01 34 87 19 05 01 34 87 19 06
JPD 17/07/07
Sidena 24/06/2007
User Manual
Ver 1.2
_____________________________________________________________________________________________________
Connecting to a PC
Copy the file “Biosum_XFER.EXE” to a directory in a Windows PC.
Switch the Biosum on, and strike function “XFER” in the main menu.
Launch the program “Biosum_XFER” on the PC.
The PC tries to connect to Biosum. If it succeeds, one sees “connected” displayed at the top of the PC
screen and on the Biosum screen. If not, there is a warning message. In this case, check that the right
serial port is selected ; if necessary change it, and click again the button “Connect”.
TRANSFERT
Wait for connection…
If connection can not succeed,
Switch power OFF, then switch ON
To finish the transfer and to send Biosum back to its main menu, click the button “Disconnect”, or exit
the PC program.
The PC Program shows the operating mode of the Biosum, and the last counted key.
On the PC screen, the “TRANSFERS” group gives the possibility to :
- receive data from Biosum
- transfer these data to an Excel spreadsheet
- erase the data in Biosum memory
- export the data as a text file.
The “TOOLS” group allows to :
- set Biosum date and time : the PC date and time are then automatically transferred.
- modify the names and mnemonics of the Biosum grid cells.
Changing names and mnemonics
The window which appears when striking button “Edit Grid” shows the mnemonics and the full names
of the cells. It also displays the last counted key, that is to say the last key that increases the total
number ; the following keys will not (see above the 3 operating modes). This key can be changed
only when in the hematology mode and it must remain beetweem 19 and 24.
While clicking “modify”, one can change all the elements of the grid. One must then transfer the new
values to the Biosum by clicking button “Send to Biosum”. These sets of names can also be saved
onto the disc and be reloaded. Each of the three operating modes can have its owm set of names
saved on disk.
SIDENA
302, avenue de Neuville 78950 Gambais
[email protected] 01 34 87 19 05 01 34 87 19 06
JPD 17/07/07