Download GIBCO Mouse (C57) MSCs

Transcript
Table of Contents
Contents and Storage........................................................................................... iv
GIBCO® Mouse (C57BL/6) Mesenchymal Stem Cells .....................................1
Methods............................................................................................... 3
Handling GIBCO® Mouse (C57BL/6) MSCs .....................................................3
Thawing and Establishing Cells ..........................................................................5
Subculturing Cells..................................................................................................8
Freezing Cells........................................................................................................10
Differentiation Media ..........................................................................................12
Differentiating GIBCO® Mouse (C57BL/6) MSCs ..........................................14
Osteogenic Differentiation..................................................................................15
Adipogenic Differentiation.................................................................................17
Chondrogenic Differentiation ............................................................................19
Appendix ........................................................................................... 21
Troubleshooting ...................................................................................................21
Additional Products.............................................................................................23
Technical Support ................................................................................................24
Purchaser Notification.........................................................................................25
References..............................................................................................................27
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