Download Plasmid DNA Purification

Plasmid DNA Purification
Table of contents
1 Kit contents
5
2 Kit specifications
7
3 About this user manual
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4 NucleoBond® Xtra plasmid purification system
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4.1 Basic principle
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4.2 NucleoBond® Xtra anion exchange columns
9
4.3 Growth of bacterial cultures
11
4.4 Culture volume for high-copy plasmids
12
4.5 Culture volume for low-copy plasmids
13
4.6 Cell lysis
14
4.7 Difficult-to-lyse strains
14
4.8 Filtration and loading of the lysate
15
4.9 Washing of the column
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4.10 Elution and concentration of plasmid DNA
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4.11 Determination of DNA yield and quality
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4.12 Convenient stopping points
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5 Storage conditions and preparation of working solutions
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6 Safety instructions - risk and safety phrases
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7 NucleoBond® Xtra plasmid purification
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7.1 High-copy plasmid purification (Midi, Maxi) - English
21
NEW!
7.2 High-copy plasmid purification (Midi, Maxi) - German
26
NEW!
7.3 High-copy plasmid purification (Midi, Maxi) - French
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7.4 Low-copy plasmid purification (Midi, Maxi) - English
38
NEW!
7.5 Low-copy plasmid purification (Midi, Maxi) - German
41
NEW!
7.6 Low-copy plasmid purification (Midi, Maxi) - French
45
8 Concentration of NucleoBond® Xtra eluates with the NucleoBond® Finalizers
MACHEREY-NAGEL – 03/2006/ Rev. 01
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