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10 - LIMITATIONS OF THE TEST
Insufficient or undetectable seroconversion can possibly be reported in recently vaccinated
animals, due to delayed immune response. Similarly to the official neutralising antibody
titration method and in accordance with regulations (EC) No 998/2003 effective July 3,
2004, the PLATELIA™ RABIES II test should be carried out on samples taken at least thirty
days after vaccination.
11 - EQUIPMENT AND MATERIAL REQUIRED BUT NOT SUPPLIED
Equipment
• Microplate reader equipped with 450 and 620 nm filters (*)
• Microplate incubator set at 37°C ± 2°C.
• Manual, semi-automatic or automatic microplate washer (*)
• Vortex® mixer.
(*) Contact us for detailed information about Bio-Rad instruments validated by our technical
departments.
Material
• Automatic or semi-automatic adjustable or preset pipettes or micropipettes to measure and
deliver 10 to 1000 μl and 1, 2 and 10 ml.
• Graduated cylinders of 25 ml, 50 ml, 100 ml, and 1 000 ml capacity.
• Disposable tubes.
• Distilled or deionized water.
• Sodium hypochloride (bleach).
• Absorbent paper.
• Goggles or safety glasses.
• Disposable latex gloves.
• Container for biohazard waste.
12 - PRECAUTIONS
1. Precautions
The quality of the results depends on correct implementation of the following good
laboratory practices:
• Reagents must be stored at +2°C to +8°C.
• Do not use expired reagents.
• Do not mix reagents from different lots within a given test run with the exception of
generic reagents R2, R8, R9, R10.
• Before use, wait 30 minutes for the reagents to adjust to the laboratory temperature.
• Thoroughly reconstitute reagents, avoiding any contaminations.
• Do not perform the test in the presence of reactive vapors (acids, alkalis, aldehydes)
or dust that could alter enzyme activity of the conjugate.
• Use glassware thoroughly washed and rinsed with deionized water or preferably,
disposable material.
• Do not leave the microplate more than 5 minutes between the end of washing
operation and the reagent distribution.
• Check the accuracy of the pipettes and the good operation of the apparatus used.
• Never use the same container to distribute conjugate and the development solution.
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