Download For Reference Purpose Only!

CircuLex
Human YKL-39 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
On
ly!
TM
Inter-assay Precision (Precision between assays)
Two samples of known concentration were tested in five separate assays to assess inter-assay
precision.
• Inter-assay (Run-to-Run, n=5) CV=5.0 - 7.4 %
Human YKL39 conc. (pg/ml)
Pu
1
2
3
4
5
MAX.
MIN.
MEAN
S.D.
C.V.
Sample 2
909.0
863.9
797.4
822.5
860.4
909.0
797.4
850.6
42.7
5.0%
rp
os
e
Sample 1
347.9
286.1
305.5
313.8
301.2
347.9
286.1
310.9
23.0
7.4%
3. Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of
human YKL-39 were serially diluted with the Dilution Buffer to produce samples with values within the
dynamic range of the assay.
ce
Linearity
900
en
700
600
500
400
er
Human YKL-39 conc. (pg/mL)
800
300
200
ef
100
0
rR
0
Fo
Cat#: CY-8087
0.2
0.4
0.6
0.8
1
Sample dilution ratio
12
Version#: 120710