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Figure 7 Electropherogram of GeneScan-500 run under denaturing conditions Using the Standard Sizing Curve The Standard Sizing Curve is a measure of how well the standard definition matches the GeneScan size standard, and whether or not it is linear. To align the data by size, GeneScan calculates a best-fit least squares curve for all samples. This is a third-order curve when you use the Third Order Least Squares size calling method. For all other size calling methods it is a second-order curve. 32
