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The 100-well Rotor-Disc layout for a 23-gene panel (one disc for one sample) has 4 replicates of 23 genes and a multi-copy reference assay (MRef), arranged as shown in Figure 4. Figure 4. Disc layout for a 23-gene panel on a 100-well Rotor-Disc. One Rotor-Disc can be used to assay one sample. qBiomarker Copy Number PCR Handbook 08/2012 21