Download qBiomarker Somatic Mutation PCR Array System

Contents
Kit Contents
5
Shipping and Storage
7
Intended Use
7
Product Warranty and Satisfaction Guarantee
7
Technical Assistance
7
Safety Information
8
Quality Control
8
Introduction
9
Principle
9
qBiomarker Somatic Mutation PCR Arrays
10
qBiomarker Probe Mastermix
11
Procedure
11
qBiomarker Somatic Mutation PCR Array plate layout
12
Controls
14
Equipment and Reagents to Be Supplied by User
15
Important Notes
16
General precautions
16
DNA purification
16
PCR setup
17
Data analysis
17
Protocols
 Real-Time PCR Using the qBiomarker Somatic Mutation PCR Array
A, C, D, and F Formats
18
 Real-Time PCR Using the qBiomarker Somatic Mutation PCR Array
E and G Formats
22
 Real-Time PCR Using the qBiomarker Somatic Mutation PCR Array
E and G Formats 384HT Option
29
 Real-Time PCR Using the qBiomarker Somatic Mutation PCR Array
R Format
33
 Real-Time PCR Using the qBiomarker Somatic Mutation PCR Assay 37
Troubleshooting Guide
41
Appendix A: Whole Genome Amplification of Genomic DNA
43
qBiomarker Somatic Mutation PCR Handbook 08/2012
3