Download CMS_065522 - Applied Biosystems

Transcript
Index
Symbols
.fsa sample files 38, 51
.hid sample file 50
+A nucleotide addition
defined 80
efficiency of 80
Numerics
3100/3100-Avant instruments 31
3500/3500xL instruments 33
3730 instrument 33
A
accuracy and reproducibility 66
alleles
low frequency 95
off-ladder 67
allelic ladder
figure 13
requirements for accurate genotyping 30
volume per reaction 32, 34, 36
analysis method 42, 55
analysis method, create 42
analyze a project 48, 62
artifacts in data 81
B
bins
import 51
bins, import 38, 51
biohazard safety 114
blood samples 11, 19, 21
Bode Buccal DNA Collector 11, 19, 23
buccal samples 11, 19, 21, 23
buccal swabs 25
C
chemical safety 114
concordance studies 99
contents of kit 16
control DNA, about 17
Copan
FLOQSwabs 87
treated cards 11
D
data collection software 15
data, accuracy, precision, and reproducibility of 66
data, artifacts 81
data, for different populations 88
developmental validation 66
DNA
effect of quantity, figure 85
negative control sample preparation 21, 23, 26
positive control sample preparation 21, 23, 26
sensitivity 83
your sample preparation 21, 23, 26
documentation, related 121
E
electropherogram
causes of extra peaks 75
extra peaks 75
species specificity 82, 86, 87
electrophoresis
Data Collection Software 31, 33
data collection software 35
prepare samples 32, 33, 35
references 31, 33, 35
run module 31, 33, 35
set up of 3100/3100-Avant instruments 31
set up of 3500/3500xL instruments 33
set up of 3730 instrument 33, 35
emission spectra 16
equipment, not included with kit 105
extra peaks, causes 75
characterization of loci, validation 81
AmpFlSTR® Identifiler® Direct PCR Amplification Kit User Guide
125