E. Assay Diagram Recommended Plate Layout: Key: Blank = Buffer Only Total Binding = Biotin-PTH only Standard 1 = 1000 pg/ml Standard 2 = 100 pg/ml Standard 3 = 10 pg/ml Standard 4 = 1 pg/ml Standard 5 = 0.1 pg/ml Pos Control = Biotin with Item M 13
