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BD RevTetTM System User Manual Table of Contents continued List of Figures Figure 1. Mechanism of RevTet-On and RevTet-Off gene expression 5 Figure 2. Packaging of infectious, replication-incompetent retroviral particles 6 Figure 3. Figure 4. Establishing an inducible cell line with the RevTet System Infection of 3T3 cells with pRevTet-Off-IN produces a higher percentage of highly inducible clones 8 9 Figure 5. Overview of the RevTet procedure 13 Figure 6. Dose-response curve for the CHO-AA8-Luc Control Cell Line 19 Figure 7. Fold induction of luciferase activity in different lots of FBS 19 Figure 8. pRevTet-On Vector map 32 Figure 9. pRevTet-Off Vector map 33 Figure 10. pRevTet-Off-IN Vector map 34 Figure 11. pRevTRE Vector map and MCS 35 Protocol # PT3223-1 Version # PR33666 www.bdbiosciences.com BD Biosciences Clontech 3