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Protocol for RayBio® Human Acute Kidney Injury
Antibody Array G-Series 1
TABLE OF CONTENTS
I.
Introduction····················································································1
II.
Product Information·································································4
A. Storage Recommendations·······································4
B. Materials Provided····························································5
C. Additional Materials Required·································5
D. How It Works ·······································································6
E. RayBio® G-Series Glass Chip Layout ·············6
III.
Helpful Tips and General Considerations··············7
A. Preparation and Storage of Samples················7
B. Handling Glass Chips····················································8
C. Incubations and Washes············································8
D. Data Extraction Tips·······················································9
IV.
Protocol··························································································9
A. Preparation and Storage of Reagents············9
B. Blocking and Incubations·········································10
C. Fluorescence Detection············································14
V.
Interpretation of Results···················································15
A. Explanation of Control Spots·······························15
B. Typical Results using G-Series Arrays·········16
C. Background Subtraction··········································17
D. Normalization of Array Data··································17
E. Threshold of Significance·········································18
VI.
Antibody Array Map······························································19
VII.
Troubleshooting Guide·······················································20
VIII.
Selected References····························································22
RayBio® Cytokine Antibody Arrays are patent-pending technology.
RayBio® is the trademark of RayBiotech, Inc.
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RayBio® Human Acute Kidney Injury Antibody Array G-Series 1