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Download DNA IQ System-Small Sample Casework Protocol Technical Bulletin
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tb296.1210:EIVD_TM.qxd 3. 11/28/2010 8:09 PM Page 5 Sample Types Examined DNA from the following sample types have been successfully purified at Promega or by external forensic laboratories. Due to the nature of casework samples (i.e, the samples may have been exposed to environmental factors for long periods of time and the amount of biological material may be limiting), DNA yields may vary, and DNA may not be obtained from all samples. Please see the most updated list at: www.promega.com/dnaiqsamples/. Tissue masses, including hair, bone, and sperm, require a proteinase K digestion to obtain reliable amounts of DNA. Contact Promega Technical Services ([email protected]) for the latest information on available protocols. Table 1. Types of Samples From Which DNA Has Been Successfully Isolated Using the DNA IQ™ System. Sample Type Fresh blood Frozen blood Bloodstains S&S 903 paper FTA® paper Cotton Blue denim Black denim Soil Leather Surface to swab Buccal swabs Cotton Rayon CEP paper Swab to FTA® paper Foam swab to paper Cigarette butt Promega External Comments Yes Yes Works with the following anti-clotting reagents: EDTA, citrate, heparin, ACD. Yes Yes Old blood may produce lower yields. Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Toothbrush Yes Envelope Yes Urine Yes Use paper wrapping; filter may form gel if heated with prepared Lysis Buffer. Soak bristles in prepared Lysis Buffer at 60°C for 30 minutes. Soak in 0.5% SDS before adding 2 volumes of prepared Lysis Buffer. Sample from bladder cancer patient. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Revised 12/10 Part# TB296 Page 5
