Download 21200 - Protocol (50 prep)

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Transcript 
Flow Chart 1
Procedure for Differential Red Blood Cell (RBC) Lysis
Collect Blood in 4.8mM EDTA
Add 5 Volumes of RBC Lysis Buffer.
Vortex and incubate for 3-5 minutes.
1. Centrifuge to pellet cells
2. Gently decant supernatant
Add 2 Volumes of RBC Lysis Buffer.
Vortex
1. Centrifuge to pellet cells
2. Gently decant supernatant
White Leukocyte Pellet
3
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