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CONTENTS 3.21.Fluorescence separation unmixing (Spectral type) - Part 2: In case that control sample is used 3.22.Fluorescence separation 45 unmixing (Spectral type) - Part 3:In case that number of fluorescent dye kinds only is known (Blind Unmixing) - 47 3.23.Image save 48 3.24.Save to CD-R 49 3.25.System shut down 50 Annex 1 Relationship between confocal principle and tuning mechanism 51 Annex 2 Image acquisition of less noises 52 Annex 3 Method to change width or position of slit in manual mode 53