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Kit Contents
Catalog #
PD1222-00
PD1222-01
PD1222-02
Preps
4
50
250
ezBind Columns
4
50
250
Buffer A1
2.5 mL
25 mL
125 mL
Buffer B1
2.5 mL
25 mL
125 mL
Buffer N3
400 µL
5 mL
25 mL
Buffer KB
2.5 mL
30 mL
135 mL
Buffer RET
4 mL
50 mL
250 mL
DNA Wash Buffer*
2 mL
15 mL
3 x 24 mL
Endofree Elution Buffer
1 mL
10 mL
30 mL
0.25 mg
(17.5µL)
2.5 mg
(175 µL)
12.5 mg
(625 µL)
1
1
1
RNase A(20 mg/mL)
User Manual
*Add 8 mL (PD1222-00) or 60 mL (PD1222-01) or 96 mL (PD1222-02) 96100% ethanol to each DNA Wash Buffer bottle before use.
Safety Information


Buffer N3 contain acetic acid, wear gloves and protective eyewear when
handling.
Buffer N3, KB and RET contains chaotropic salts, which may form
reactive compounds when combines with bleach. Do not add bleach or
acidic solutions directly to the preparation waste.
Biomiga EZgeneTM EndoFree ezFlow Plasmid Miniprep Kit II Page 5