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Touchscreen Robotic needle arm Washing station 2D code reader Rack detector Fluidics Syringe pumps MACS Enrichment Unit Main switch Figure 1.1: Front view of MACSQuant Instrument. The key accessible components of the MACSQuant Instrument are: • A n integrated computer controlled by a touchscreen runs the MACSQuantify™ Software, which is used for sample acquisition as well as for data analyses. • A fully automated computer-controlled robotic needle arm delivers samples to the sample injection port, which is located in the washing station. The uptake needle as well as the sample injection port are automatically washed during and after cell processing for your convenience. • The MACS Single Tube Rack is used for the analysis of individual samples. It offers the start of your analysis by the push of a button. • The optional MACS MiniSampler allows fully automated multiple sample processing. It holds different tube racks that are automatically recognized by a 2D code reader, as well as the MACS Reagent Rack 4. • A color-coded and easy to access fluidics system supplies the MACSQuant Instrument with all buffers and solutions required during and after operation. The sheath particle filter prevents any debris from entering the fluidics system. • Bottles for Running Buffer, Washing Solution, and Storage Solution are placed in bottle holders. Liquid waste is collected in a separate waste bottle. All fluid levels are constantly monitored and visualized by the illumination of the fluid bottles. • The MACS Enrichment Unit with the optional MACSQuant Column allows easy analysis of particularly challenging cell samples, such as rare cell populations in high volumes of buffer, or precious low volume samples. 58