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Little Dipper® Processor
USER MANUAL
CAT. #1080-XX-1 (115V); 1080-XX-2 (230V)
For FISH
Cat.# 1080-70-1/2 (115V/230V)
For Illumina® BeadChip® Processing
Cat. #1080-30-1/2 (115V/230V)
For Agilent Arrays
Cat. #1080-40-1/2 (115V/230V)
For Roche NimbleGen Arrays
Cat. #1080-80-1/2 (115V/230V)
For Self Spotted Arrays
Cat. #1080-50-1/2 (115V/230V)
FOR RESEARCH USE ONLY
Not for Use in Diagnostic Procedures
Manufactured by:
6FL*HQH‡/DNHVLGH'U6WH)6XQQ\YDOH&$86$‡‡WHFKVHUY#VFLJHQHFRP
Little Dipper® Processor — USER MANUAL
Serial Number
The following serial number identifies the specific instrument you have purchased
and must be referenced when requesting service. A copy is affixed to the
instrument.
Technical Service: (408) 733-7337, [email protected]
H
Warranty
B
SciGene warrants that the Little Dipper® Processor described in this manual shall
be free of defects in materials and workmanship for a period of 12 months from
date of delivery. This warranty does not cover removable parts or accessories,
including removable baths. In the event of a defect during the warranty period,
SciGene’s limit of liability will be to provide repair service at its factory at no
charge or, at its sole discretion, replace the product. All original packaging should
be retained by the customer in the event that the instrument is returned to the
factory for service or repair. The foregoing warranty is void in the event the unit
was abused or modified or used in a manner inconsistent with its intended
purpose. SciGene makes no other warranty, expressed or implied including
warranties of merchantability and fitness for a particular purpose. In no event shall
SciGene be liable for any direct, indirect, special, incidental or consequential
damages or for any damages resulting from loss arising out of or in connection
with the sale, use or performance of the product.
Copyright
Copyright ©2005-2010 SciGene Corporation. All rights reserved. SciGene and Little
Dipper are trademarks of SciGene Corporation, Sunnyvale, California, USA. All
other trademarks used in this manual are the property of their respective owners.
Intended Use
The intended use of this instrument is for washing and drying of microarrays and
fluorescent in situ hybridization (FISH) slides.
This instrument should only be used according to instructions provided in this
User Manual and other SciGene technical documents. If the equipment is used in a
manner not specified by the manufacturer, the protection provided by the
equipment may be impaired.
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Table of Contents
I.
SAFETY NOTICES............................................................................................................................................................3
A.
Instrument Safety ...................................................................................................................3
B.
Conventions and Labels..........................................................................................................3
C.
Spills and Reagent Disposal ....................................................................................................4
II.
SITE PREPARATION .......................................................................................................................................................6
III.
UNPACKING AND SETUP.............................................................................................................................................7
A.
Unpacking and Verifying Items ............................................................................................... 7
B.
Identifying System Components .............................................................................................8
C.
Computer and Power Connections ..........................................................................................9
IV.
SYSTEM OPERATION..................................................................................................................................................10
A.
Installing Baths and Centrifuge Buckets ................................................................................10
B.
Using the Touch Screen ........................................................................................................11
C.
Using the Pause Button.........................................................................................................12
D.
Using the Emergency Stop .................................................................................................... 12
E.
Running a Test Protocol / Practicing Rack Loading ...............................................................12
F.
Running a Pre-Installed Protocol ...........................................................................................14
G.
Using the Temperature Controllers.......................................................................................17
V.
CUSTOM PROTOCOLS...............................................................................................................................................18
A.
Adding a Protocol .................................................................................................................18
B.
Deleting a Protocol ...............................................................................................................19
VI.
PERIODIC MAINTENANCE........................................................................................................................................21
A.
Cleaning Before Every Use ....................................................................................................21
B.
Cleaning After Every Use.......................................................................................................21
C.
Monthly Cleaning and Calibration.........................................................................................21
VII.
SYSTEM CALIBRATION..............................................................................................................................................22
A.
Temperature Controller Calibration ......................................................................................22
B.
Slide Rack Position Calibration..............................................................................................24
C.
Centrifuge Loading Calibration ............................................................................................. 26
D.
Touch Screen Calibration......................................................................................................26
VIII. SPECIFICATIONS ..........................................................................................................................................................28
IX.
ORDERING INFORMATION ......................................................................................................................................29
X.
TROUBLESHOOTING ..................................................................................................................................................30
XI.
SERVICE............................................................................................................................................................................31
A.
Lockout Procedure................................................................................................................31
B.
Fuse Replacement.................................................................................................................31
XII.
DECLARATION OF CONFORMITY ........................................................................................................................32
Manual 1.6, Software 1.4 June 2010
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Little Dipper® Processor — USER MANUAL
I.
SAFETY NOTICES
A. Instrument Safety
Before operating the instrument, read the information in this section
concerning hazards and potential hazards. Ensure that everyone involved
with the instrument’s operation is instructed in both general safety
practices for laboratories and specific safety practices for the instrument.
B. Conventions and Labels
The following is a glossary of the safety conventions and symbols that are
used on the Little Dipper® Processor. Whenever such symbols appear on
instruments, please observe appropriate safety measures.
1. Warnings
Failure to comply with the following warnings that are affixed to the
product can lead to possible personal injury or death.
This symbol indicates the potential for a Pinch
Point. Do not place fingers in the slot in the
Z-axis tower.
This symbol indicates the potential presence of a
Hot Surface. Turn off the temperature controller
and leave sufficient time for cooling or use heat
protection prior to handling a bath.
Biological hazards can be associated with the
materials used on this instrument. Potentially
infectious waste and other hazardous waste must
be treated in accordance with good laboratory
practice guidelines.
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2. Cautions
Failure to comply with the following cautionary statement affixed to
the product may lead to possible personal injury.
Do not touch the bottom metal surface of the bath
during operation. Insert the bath liner and fill with
buffer before turning on power to each bath.
The Little Dipper System weighs approximately
59 lbs (26.5 kg). Use caution when lifting the unit
to avoid personal injury.
Do not under any circumstances lift the unit by the
cylindrical tower or its extended gripper arm!!
This will damage the instrument!
Do not under any circumstances run a protocol while
a bath cover is in place.
To achieve temperatures above 70˚C, a bath cover
must be used to obtain and maintain temperatures.
When the robot is in use, baths running at high
temperatures may need to be topped off due to
evaporation.
C. Spills and Reagent Disposal
Observe all national, regional, and local regulations
for waste disposal and management.
Biological and other hazards can be associated with the
materials used on this instrument. Potentially infectious
and other hazardous waste materials must be treated in
accordance with good laboratory practices.
This symbol indicates the potential presence of a Hot
Surface. Use care when cleaning a spill in this area
to avoid being burned.
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1. Cleaning Spills
a. Turn off the instrument.
b. Wipe the spill using an absorbent material. Always pull the
liquid to the front of the instrument, away from the control
panel and around the bath positions.
c. Clean the deck with a cloth or paper towel and an
appropriate disinfectant solution (e.g. 70% ethanol) for the
type of spill present.
d. Wipe the deck dry.
e. Dispose of the material in a manner that is in accordance
with good laboratory practices.
2. Disposing of Reagents
Some chemicals used on this instrument can be hazardous.
Please adhere to good laboratory practices when disposing of
hazardous chemicals.
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II.
SITE PREPARATION
The Little Dipper Processor requires a level surface area of at least 30x22
inches (76 x 56 cm) with a vertical clearance of 30 inches (76 cm) (Fig. II-1).
Locate the unit within 3 feet (1 meter) of a single power outlet that delivers at
least 1700 Watts/20 Amps of constant power.
Clear the benchtop for the instrument before unpacking any cartons. Allow
unimpeded access to the Emergency Off button and main power switch on
the right side of the instrument.
Fig. II-1. Allow proper clearance for the instrument.
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III.
UNPACKING AND SETUP
A. Unpacking and Verifying Items
The Little Dipper Processor is usually shipped in two cartons — a large
carton for the instrument and small carton for accessories. The
instrument weighs approximately 59 lbs (26.5 kg). Never attempt to lift
or move the instrument alone. Use caution when lifting the unit to
protect yourself and others from personal injury.
Do not lift the unit by the robot arm. Always grip the
instrument from underneath the base when lifting!!
1. Open the large carton and remove the interior box and foam.
2. With the aid of another person, carefully lift the instrument by its
base only, and place it directly on the benchtop (Fig. III-A-1).
Ensure clearance around the instrument making sure not to block
the cooling fan in the back.
3. Use scissors to cut the white tie wrap that prevents the gripper
arm from rotating during shipping (Fig. III-A-2).
4. Unpack the interior box that contains the touchscreen, power cord
and user documentation.
5. Open the smaller carton and remove the accessories and
additional components. Items correspond to the specific
configuration (catalog number) of the instrument.
6. Verify all packing list items are received in good condition.
Fig. III-A-1. Lift by the base.
Fig. III-A-2. Cut tie wrap.
Table 1. Items Provided in Shipment
INSTRUMENT Carton
Little Dipper Processor
User Manual
Power cord
Touch screen with cable
ACCESSORIES Carton
* Baths, Glass Inserts, Slide Racks
* Bath Covers
* Digital Thermometer
* Centrifuge Buckets, Pads and Spacers
*Varies by configuration.
Contact SciGene Customer Service [[email protected]] or your local
distributor regarding any discrepancy.
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B. Identifying System Components
Main
Instrument
Fig. III-B-1.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
Main Power Switch – Turns on power to the unit
Bath Power Switch – Turns on power to each bath, for heating liquid
Temperature Controller – Used to set and observe bath temperature
Temperature Sensor – Transmits bath temperature to controller.
Standard Bath – Removable insert, compatible with most liquids/solvents
Low Volume Bath - Removable stainless steel insert
Glass insert – Protects stainless steel from stir bar abrasion
Gripper Arm – Rotates and moves slide racks up and down in baths
Gripper – Opens, closes and secures the slide rack assembly
Fan – Draws air through the instrument cabinet for cooling
Emergency Switch (EMO) – Cuts power to the instrument
Slide Rack(s) – Holds slides during processing.
Slide Rack Handle – Attaches to
Hybex and 24-position slide racks.
Centrifuge Access Panel –
Internal View
Automatically opens for the gripper
of Centrifuge
arm to insert a slide rack
Centrifuge Latch – Keeps lid closed
Touch screen Cable Connection –
Receptacle for attaching control cable
Touchscreen – Used to create, store
and run protocols and for calibration
Centrifuge Swinging Buckets – Red
(balance) and green (sample) buckets
are removable. Sample bucket receives
slide rack from gripper.
Temperature Calibration Connection
- Receptacle for cable to thermometer
Power Cord Connection - Receptacle
for power cord
Stirbar Speed Control – Rotating
switch to adjust stirbar speed
Stirbar – Spinning bar that creates a
Fig. III-B-2.
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gentle vortex in the bath
C. Computer and Power Connections
1. Connect the braided cable to the touchscreen and to the
receptacle labeled “TOUCHSCREEN” on the right side of the
instrument, using the circular connectors (Fig. III-C-1).
2. Connect the power cord to the receptacle labeled “POWER” on the
right side of the instrument (Fig. III-C-2) and to a power source
that delivers 15 Amps or more for 115V units or 8 Amps or more
for 230V units. Avoid connecting to a circuit with a centrifuge,
freezer or other equipment that draws significant power.
3. Turn on the main power switch on the right side of the
instrument. The touchscreen should illuminate and the motors
should activate (Fig. III-C-3).
4. Briefly turn on power to each of the five baths using the power
switch located above each bath position (Fig. III-C-4). Verify that
each temperature controller illuminates and then turn off power to
each bath position.
Baths should not be powered on for more than a few
seconds without buffer in the bath inserts.
Do not heat dry baths.
Fig. III-C-1. Connect touchscreen.
Fig. III-C-2. Connect power cord.
Fig. III-C-3. Turn on main power.
Fig. III-C-4. Briefly turn on baths.
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IV.
SYSTEM OPERATION
A. Installing Baths and Centrifuge Buckets
1. Clean bath inserts, racks and stir bars by rinsing in running tap
water, followed by 100 ml of DI water and then 100 ml of ethanol.
Dry with a lint-free towel (Fig. IV-A-1).
2. Clean sensors with DI water and a lint-free towel (Fig. IV-A-2).
3. According to the instrument configuration, install standard or
heatable low volume baths by first rotating temperature sensors
up, placing baths and then lowering sensors. For low volume
baths without temperature control, leave sensors down while
placing baths (Fig. IV-A-3).
4. Place glass inserts in all low volume baths (Fig. IV-A-4).
5. Place a stir bar in all baths. Note that low volume baths use low
profile stir bars (SciGene cat. #1080-11-1) (Fig. IV-A-5).
Fig. IV-A-1. Clean baths and racks.
Fig. IV-A-3. Add removable baths.
Fig. IV-A-2. Clean sensors.
Fig. IV-A-4. Add glass inserts.
6. Unlock and open the centrifuge.
7. Insert a red bucket by hanging it on the round tabs on the rotator
arms marked “BALANCE RACK”. Repeat using a green bucket on the
rotator arms marked “SAMPLE RACK” (Fig. IV-A-6).
8. Place an absorbent pad in the bottom of each bucket and put a
black frame spacer on top (Fig. IV-A-7).
9. Balance the centrifuge by inserting a rack with the same number
of slides as the sample rack into the red bucket (Fig. IV-A-8).
10. Close and lock the centrifuge (Fig. IV-A-9).
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Fig. IV-A-5. Add stir bars.
Fig. IV-A-6. Insert buckets.
Fig. IV-A-7. Insert pads and spacers.
Fig. IV-A-8. Load balance rack.
Fig. IV-A-9. Lock centrifuge.
.
B. Using the Touch Screen
Use the handheld touchscreen to navigate between screens, select
and run protocols, create new protocols and control instrument
movement (Fig. IV-B-1). If the touchscreen fails to respond, it may
require calibration. See Section VI C. for details.
Fig. IV-B-1. Use touchscreen controls.
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Fig. IV-C-1. Pause protocols as needed.
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C. Using the Pause Button
The action of the instrument can be immediately stopped and restarted
using the illuminated red “PAUSE/RESUME” button at top right on the
touchscreen. When pressed, the instrument will stop and the button light
will flash. To resume the protocol, press the button again (Fig. IV-C-1).
D. Using the Emergency Stop
To immediately stop movement of the gripper arm and stir bars, press
the red Emergency Stop (EMO) button on the right side of the instrument
(Fig. IV-D-2). Bath heaters will continue to receive power. To restore
power to the gripper arm and stir bars, release the EMO button by
rotating it a quarter turn clockwise (Fig. IV-D-2). To move the arm, press
Control Panel on the Home Page and use the up | down | left | right
buttons. To remove the slide rack, hold the rack and press the Open
Gripper button on the Control Panel screen. The protocol will not
resume upon releasing the EMO switch and must be restarted from the
beginning.
If the touch screen does not illuminate when the unit is
turned on, rotate the red EMO button on the side of the
instrument to release the emergency stop.
Fig. IV-D-1. Press EMO to stop.
Fig. IV-D-2. Rotate to restore power.
E. Running a Test Protocol / Practicing Rack Loading
This test verifies the mechanical operation of the instrument and
provides the operator an opportunity to practice loading the slide rack
onto the gripper. An empty slide rack is automatically moved from bath
to bath with agitation, followed by a brief centrifugation. No liquid is
required for this test and temperature controllers should remain OFF.
1. Ensure that baths and buckets are properly installed (see section
IV. A. Installing Baths and Centrifuge Buckets) and correspond
to the size of the slide racks to be used. Verify that the all
temperature sensors are rotated down.
2. Turn on main power located on the right side of the instrument.
3. Using the touchscreen, press Run Protocol from the home screen
then select the Quik-tst protocol (Fig. IV-E-1).
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4. Hold an empty slide rack centered in Bath #1, aligned with the
black lines on the instrument and slightly below the bath fill line.
5. Start the protocol by pressing Start Now in the lower right corner
of the touchscreen. The gripper arm will lower into Bath #1 with
the gripper open. Align the holes in the handle with the two
bumps in the gripper paddle as the gripper closes (Fig. IV-E-2).
If the gripper closes and the rack is not held securely, press the
red EMO switch immediately (Fig. IV-D-1). Release the rack, if
needed, by pressing Open Gripper from the Control Panel
screen, then select and start the protocol again.
Immediately press the EMO switch to stop the
instrument, if the slide rack fails to mount on the
gripper properly or touches the sides of the bath.
6. The instrument will now agitate the rack in each bath.
7. Observe the placement of the slide rack in the baths. The rack will
automatically move from the last bath into the centrifuge and spin
for a minute.
If the rack touches the sides of the baths, consult section VII. B.
Slide Rack Position Calibration.
8. When the protocol is complete, unlock and open the centrifuge lid
to remove the rack from the sample bucket.
Fig. IV-E-1. Quik-tst protocol.
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Fig. IV-E-2. Load slide rack.
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F. Running a Pre-Installed Protocol
Do not under any circumstances run a protocol while
a bath cover is in place.
To achieve and maintain temperatures above 70˚C,
a bath cover must be used, while the machine is NOT
in motion. Baths at high temperatures may need
buffer(s) added to offset evaporation.
The instrument comes configured with validated protocols installed.
Refer to the Little Dipper Processor Method corresponding to the
microarray or FISH protocol to be performed for details on how to set up
the instrument. Method sheets can be found online [www.scigene.com/
support/protocols] or can be requested from SciGene Customer Service
[[email protected]] or your local distributor.
The following are general guidelines on how to set up and run a preinstalled protocol.
1. Ensure baths, racks and stir bars are free of buffer residue and
dust. See section VI. A. Cleaning Before Every Use.
2. Insert baths, glass inserts (for low volume baths) and stir bars as
specified for the protocol (Fig. IV-F-1).
3. Rotate all temperature sensors down (Fig. IV-F-2).
Fig. IV-F-1. Place baths and stir bars.
Fig. IV-F-2. Rotate sensors down.
Fig. IV-F-3. Turn on main power.
Fig. IV-F-4. Set temperature(s).
4. Add recommended buffer(s) to the fill line.
5. Turn on main power to the instrument (Fig. IV-F-3).
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6. Turn on power to bath(s) to be heated and set temperature(s) (Fig.
IV-F-4). Cover any baths set above 70°C. Allow time for baths to
reach set temperature(s).
7. Place a balance rack into the centrifuge with same number of
slides as the sample rack (Fig. IV-F-5).
8. Add microarray or FISH slides to sample rack. To insert microarray
slides, submerge the sample rack in a glass dish filled with the same
buffer to be used in Bath #1. Remove slides from the hybridization
cassette and insert into the submerged rack (Fig. IV-F-6).
9. Remove any bath covers.
10. Move the sample rack to Bath #1.
11. On the touchscreen home page, press Run Protocol, then select
the desired protocol and press Start Now (Fig. IV-F-7).
12. Carefully lift the sample rack and load onto gripper (Fig. IV-F-8).
The instrument will perform the steps as programmed.
13. At completion (depending on the protocol), remove the rack from
the centrifuge (Fig. IV-F-9) or from above the last bath (Fig. IV-F-10),
if running a protocol without centrifugation, by pushing the
thumbpad to open the gripper paddle.
14. Clean the instrument per section VI. B. Cleaning After Every Use.
Fig. IV-F-5. Insert balance rack.
Fig. IV-F-6. Add slides to sample rack.
Fig. IV-F-7. Press Start Now.
Fig. IV-F-8. Load sample rack.
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Fig. IV-F-9. Remove rack (option 1).
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Fig. IV-F-10. Remove rack (option 2).
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G. Using the Temperature Controllers
Temperature controllers are used to regulate heating of the five bath
positions. Each controller is calibrated at the factory to provide an
accurate bath temperature when operated between 35 to 90°C. The
following instructions are general guidelines for setting temperature(s).
1.
Select a bath position (according to your protocol) and turn ON the
switch above the bath to provide power to the temperature
controller. The controller will display current temperature.
2.
Press and hold the SET button to view the previous set temperature.
Baths should not be powered on for more than a few
seconds without buffer in the bath inserts.
Do not heat dry baths.
Do not touch the bottom metal surface of the bath
during operation. Insert the bath liner and fill with
buffer before turning on power to each bath.
This symbol indicates the potential presence of a
Hot Surface. Turn off the temperature controller
and leave sufficient time for cooling or use heat
protection prior to handling a bath.
3.
To change the set temperature, press and hold the SET button, then
press the up or down arrow buttons until the desired temperature is
shown. Release the SET button. Allow time for the bath to reach the
set temperature and stabilize.
Refer to section VII. A. Temperature Controller Calibration for
information on calibrating the controllers.
Fig. IV-G-1. Temperature controllers.
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Fig. IV-G-2. Set temperature.
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V.
CUSTOM PROTOCOLS
A. Adding a Protocol
Custom protocols can be created using the touchscreen. The
touchscreen can store up to 14 protocols with 1 to 9 steps each.
1. From the touchscreen Home page, press New/Edit Protocol. On
the Create/Edit Protocol screen (Fig. V-A-1)., use the up/down
buttons to move the cursor “»” to “NEW”. Press Select to go to the
Name Protocol screen (Fig. V-A-2).
2. Within the Name Protocol screen, use the shift key to move the
“^” (up arrow) within the numeric 10 key pad until the desired
letter is indicated. Press to select the indicated letter displayed
above the key pad. Continue for up to eight characters.
3. When finished, press Enter to go to the Step Input screen (Fig. VA-3) and then press Step 1.
4. When the Parameters screen displays, press the Bath # button,
enter “1”, and then press Enter.
Fig. V-A-1. Create/Edit Protocol screen.
Fig. V-A-2. Name Protocol screen.
Fig. V-A-3. Step Input screen.
Fig. V-A-4. Step 2 Parameters screen.
5. Select the remaining parameters for Step 1 of the protocol and
then enter the desired values within the ranges indicated.
All protocols start from Bath #1.
Table 2. Parameter Settings for Custom Protocols
Parameter
Agitation Rate
Agitation Time
Stroke Length
Pause Time1
Manual 1.6, Software 1.4 June 2010
Range
0-600
0-32,000 seconds
Standard or Long
0-32,000 seconds
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1 Time the instrument pauses at the end of the step with rack remaining submerged.
2 After agitation, time that the rack is suspended above bath, before moving
6. To add a Pause within the protocol, do one of the following:
•
•
Enter time for rack to remain submerged at end of step
Enter 9999 for User Control. Rack will stay submerged until
the operator presses the touchscreen to resume the protocol.
This is beneficial for protocols that require more than 5 baths.
7. When all values for Step 1 are entered, press Save and Exit to
return to the Step Input screen.
8. Continue to enter values for additional steps (max. 9) (Fig. V-A-4).
Remember to program “C” for the centrifuge, if ending in a drying
step.
9. When finished creating the protocol, press Save & Exit to return to
the Select Protocol screen. Up to 3 steps are displayed at one time
on the touch screen. Each screen of the protocol must be saved
separately before saving the entire protocol.
B. Deleting a Protocol
1. To delete a protocol, press Control Panel from the home screen
then Advanced Settings.
2. Press the Delete Protocol button to open the screen.
3. Choose a protocol to delete using the up and down arrows and
then press Select. A confirmation screen appears saying “Delete
Protocol?” Choose YES to confirm.
4. Press the BACK button on each of the previous screens to return
to the home screen.
Fig. V-B-1. Advanced Settings screen.
Fig. V-B-2. Delete Protocol button.
Fig. V-B-3. Delete Protocol screen.
Fig. V-B-4. Delete Protocol confirmation.
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Little Dipper® Processor — USER MANUAL
VI.
PERIODIC MAINTENANCE
The following instructions for periodic cleaning and maintenance can be
found online at www.scigene.com/support/LD_Periodic_Maintenance.pdf
A. Cleaning Before Every Use
Baths, racks and stir bars must be free of buffer residue and dust before
processing slides. If they were not cleaned and stored properly after the
last use, wash and dry them as follows:
1.
2.
3.
4.
5.
6.
Fill baths containing the rack(s) and stir bars with tap water.
Wipe racks, stir bars and baths with a lint-free towel.
Rinse in running tap water.
Rinse with approximately 100-200 ml of DI water.
Rinse with 100 ml of ethanol and dry with a lint-free towel.
Clean temperature sensors with DI water and a lint-free towel (Fig.
VI-A-1).
B. Cleaning After Every Use
1. Wash baths, stir bars and racks with running tap water then rinse
in DI water and ethanol and dry with a lint-free towel. Store in
clean plastic bags to protect from dust (Fig. VI-B-2).
2. Clean temperature sensors with DI water and lint-free towel.
3. Wipe exterior of instrument cabinet with a damp paper towel.
Fig. VI-A-1. Clean temperature sensors.
Fig. VI-B-2. Place clean baths in bags.
C. Monthly Cleaning and Calibration
1. Calibrate bath temperatures using a NIST-certified thermometer.
See section VI. A. Temperature Controller Calibration or
SciGene’s document “Bath Temperature Validation Methods for
CLIA-Regulated Laboratories”.
2. Remove and wash the centrifuge buckets and spacers using warm
water and lint-free towels.
3. Replace the absorbent pad in each centrifuge bucket.
4. Clean all exterior surfaces of the cabinet, gripper paddles and
gripper arm housing with damp, lint-free towels.
5. Remove the centrifuge buckets. Clean the inside surfaces of the
centrifuge and rotor with damp, lint-free towels.
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6. Inspect the centrifuge buckets for signs of wear or deformation
where they hang from the rotor. Replace if worn or bent.
VII.
SYSTEM CALIBRATION
The instrument is calibrated and tested at the factory and should not require
adjustment under most circumstances.
A. Temperature Controller Calibration
The temperature controllers are calibrated at the factory to provide accurate
bath temperatures from ambient +5 to 90°C. Re-calibration is
recommended only if the display varies by more than one degree (1°C) to
the temperature shown on an externally connected NIST certified digital
thermometer (Cat. #1051-52-0). The following procedure can be found
online: [www.scigene.com/support/LD-Method_Temp.Calibration.pdf].
1. Add water to bath fill line and ensure temperature sensor is down
in contact with liquid in the bath (Fig. VII-A-1).
2.
Set the temperature on the controller to 65°C and allow 20
minutes to stabilize (Fig. VII-A-2).
3. Using the cable provided with the digital thermometer, plug one
end into the blue receptacle above the bath and the other into the
thermometer (Fig. VII-A-3).
4. Turn ON the thermometer. The bath temperature will be displayed.
5. On the temperature controller, press the Infinity Key (∞) for three
seconds until “OPEN” appears.
6. Press the Down Arrow (↓) four times until “Cal” appears.
7. Press and hold the SET key. The existing offset value between the
controller and digital thermometer is displayed.
8. Calculate the difference in the temperature shown on the
controller and the digital thermometer; (e.g. the controller
displays 65°C and the digital thermometer displays 63.5°C, the
difference is -1.5°C).
9. If the controller display varies by more than one degree (1°C) from
the NIST certified digital thermometer, press and hold the SET key
and use the up and down arrows to enter the offset value
calculated in Step 7 above. For example, if the controller displays
a temperature that is 1.5°C higher than the digital thermometer,
adjust the offset value to minus 1.5 (-1.5).
10. Press the Infinity Key (∞) to exit calibration. The bath temperature
is now calibrated for the set temperature you selected. Check and
calibrate the controllers for the remaining baths, as necessary.
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Fig. VII-A-1. Fill bath.
Manual 1.6, Software 1.4 June 2010
Fig. VII-A-2. Set temperature.
23
Fig. VII-A-3. Use thermometer.
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B. Slide Rack Position Calibration
The instrument is calibrated and tested at the factory for proper rack
positioning and should not require adjustment unless a new touchscreen
is installed. The following tests check proper alignment. Baths should
remain OFF with no liquid needed.
1. Bath #1 Loading
a. Insert standard baths into Baths #1 and #2.
b. Insert and activate stir bars.
c. Hold a 12-position rack in Bath #1, aligned with the black lines
on the instrument and slightly below the fill line.
d. Using the touchscreen, press Control Panel | Advanced
Settings | Bath 1 Loading (Fig. VII-B-1). The gripper arm will
move over Bath #1, open and lower. Align the slide rack handle
onto the paddle bumps on the open gripper. The gripper will
not close.
e. While continuing to hold the rack on the open gripper, use the
left/right and up/down arrows on the touchscreen to position
the rack in the center of the bath, above the rotating stir bar
such that it does not touch the rotating stir bar.
f. When finished, remove the rack from the gripper and click
Save/Exit to save settings.
Fig. VII-B-1. Calibrate Bath 1 Loading screen.
Fig. VII-B-2. Center Sliderack in Bath 2 screen.
2. Bath #2 Center Calibration
a. Insert standard baths into Baths #1 and #2 with stir bars.
b. Activate stir bars.
c. Hold a 12-position rack in Bath #1, aligned with the black lines
on the instrument and slightly below the fill line.
d. Using the touchscreen, press Control Panel | Advanced Settings
| Bath 2 Center (Fig. VII-B-2). The gripper arm will move over Bath
#1, open and lower. Load the slide rack handle onto the paddle
bumps on the gripper as it closes. Let go of the rack.
e. The gripper arm will move the rack to Bath #2 and stop. Verify
that the rack is centered in Bath #2. Use the left/right arrows to
adjust the position if necessary.
f. Press Save/Exit when finished.
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C. Centrifuge Loading Calibration
This test ensures the rack is automatically inserted into the sample
bucket of the centrifuge.
1. Place a 12-position rack in Bath #1. In the Advanced Settings
screen, press C’fuge Loading (Fig. VII-C-1). Load the rack onto the
gripper (Fig. VII-C-1). The gripper will close and then move the rack
to the centrifuge. The centrifuge access panel opens automatically
and the gripper arm lowers the rack inside, stopping at a set
position in the green bucket.
2. Look down through the access panel to verify that the rack is
centered from side to side in the bucket. Use the arrow buttons to
adjust the position of the arm and centrifuge rotor if needed.
3. Press Save/Exit. The arm will move up and down in the centrifuge
and then release the rack. Make sure the rack gently falls into
place into the green bucket.
Fig. VII-C-1. Calibrate Centrifuge Loading screen.
Fig. VII-C-2. Rack loading into centrifuge.
D. Touch Screen Calibration
If the touchscreen fails to respond to touch, it may need calibration.
1. To access the Touchscreen Calibration screen, press the
touchscreen while the instrument is powering up.
2. Touch point 1 (Fig. VII-D-1).
3. On the Point 2 screen, touch point 2.
4. On the Point 3 screen, touch point 3 (Fig. VII-D-3).
5. After the Home screen displays, check that the screen now
responds to touch. If it does not respond, contact SciGene
Technical Service [[email protected]] or your local distributor.
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Fig. VII-D-1.Touchscreen calibration point 1.
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Fig. VII-D-2.Touchscreen calibration point 3.
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Little Dipper® Processor — USER MANUAL
VIII.
SPECIFICATIONS
Table 3. Little Dipper Processor Specifications.
Electrical
Power supply
115V AC, 50/60 Hz; 1700W; 15 Amps (cat. # 1080-XX-1)
230V AC; 50/60 Hz; 1700W; 8 Amps (cat. # 1080-XX-2)
Processing
Control
Slide capacity
Centrifuge speed
Temperature Control
Software-controlled by external touchscreen
Holds up to 24 slides per batch
690 RPM ± 15 RPM
Range: Ambient: +5 to 90°C (41 to 194°F)
Regulation: ± 0.5°C from set point
Bath volumes
Standard: 670 ml
Low volume, heatable: 270 ml
Low volume: 210 ml
standard
low volume, heatable
low volume
Environmental
Ambient temperature
Relative humidity
Overvoltage category
Operation: +15 to +32°C (58 to 90°F)
Storage: -20 to +60°C (-4 to 140°F)
Operation: 20 to 80% RH non-condensing
Storage: 10 to 90% RH non-condensing
II
Physical
Dimensions
Weight (net)
51 x 71 x 56 cm (20 x 28 x 22 inches) (H x W x D)
26.5 kg (59 lbs)
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IX.
ORDERING INFORMATION
Table 4. SciGene Ordering Information for the Little Dipper Processor.
SciGene Cat. #
Product Description
UOM
1080-10-1
Standard Bath, 670 ml.
Each
1080-10-2
Low Volume Bath, 210 ml. Includes a glass insert and stir bar.
Each
1080-10-5
Low Volume Heatable Bath, 275 ml. Includes a glass insert and stir bar.
Each
1080-10-3
Glass Insert for Little Dipper Low Volume Bath, 5/pk.
1080-11-0
Stir bars for Little Dipper standard baths, 10/pk.
10/PK
1080-11-1
Stir bars for Little Dipper low volume baths, 10/pk.
10/PK
5/PK
1080-12-0
Bath cover.
Each
1080-12-1
Bath cover, 5/pk.
5/PK
1080-20-0
Slide Rack, 24-Position for standard 3 inch slides.
Each
1080-20-1
Slide Rack, 12-Position for standard 3 inch slides. Includes handle.
Each
1080-20-2
Slide Rack, 12-Position for Illumina 3.25 inch slides. Includes handle.
Each
1080-20-3
Slide Rack, 24-Position for Illumina 3.25 inch slides.
Each
1080-21-0
Absorbent Pads for Slide Rack Buckets, Hybex and 24-position, 25/pk.
25/PK
1080-21-1
Absorbent Pads for Slide Rack Buckets, 12-Position, 25/pk.
25/PK
1080-22-1
Slide Rack Bucket for Samples, green, Hybex and 24-position racks.
1080-22-2
Slide Rack Bucket for Balance, red, Hybex and 24-position racks.
Each
1080-22-3
Spacer for Rack Bucket, 2/pk, Hybex and 24-position racks.
2/PK
1080-23-1
Slide Rack Bucket for Samples, green, 12-position racks.
Each
1080-23-2
Slide Rack Bucket for Balance, red, 12-position racks.
Each
1080-23-3
Spacer for Rack Bucket, 2/pk, 12-position racks.
1080-24-0
Handle for Hybex or 24-position racks.
Each
1080-31-0
Little Dipper Upgrade Kit for Illumina BeadChips.
Each
1080-41-0
Little Dipper Upgrade Kit for Agilent Arrays.
Each
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Each
PK
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X.
TROUBLESHOOTING
Table 5. Troubleshooting the Little Dipper Processor.
Indication
Instrument does not
power up.
Instrument is receiving
power but robot will not
respond.
Protocol does not run.
Bath temperature varies
by several degrees from
Probable Cause
Corrective Action
Power disconnected
Check power connections.
Main power switch is OFF.
Turn main power switch ON.
Fuse has blown.
Replace fuse with same type.
Emergency stop button
Twist the red EMO button on the side of the
Pause button has been
Press the red pause button on the top of the
has been activated.
activated.
instrument to release the emergency stop.
touch screen.
Temperature controller
needs to be re-calibrated.
Follow the instructions in section VII. A.
NIST-certified
Touchscreen does not
Touchscreen needs
Follow the instructions in section VII. A.
respond.
calibration
Touchscreen Calibration.
Temperature Controller Calibration.
thermometer.
Question
How do I clean the
instrument?
How do I safely move
the instrument?
How do I get help with a
custom protocol?
Answer
Refer to section VI. C. Periodic Maintenance and/or request a “Periodic
Maintenance” document (Fig. X-1) from Customer Service
[[email protected]].
Request a “Packing the Little Dipper® Processor” document (Fig. X-2) from
Customer Service [[email protected]].
Request a “Little Dipper Troubleshooting Questionnaire” (Fig. X-3) from
Customer Service [[email protected]].
Fig. X-1.Maintenance instructions.
Manual 1.6, Software 1.4 June 2010
Fig. X-2.Repackaging instructions
30
Fig. X-3.Troubleshooting questionnaire.
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XI.
SERVICE
If the need for service should occur, contact SciGene Technical Services
[[email protected]] or your local distributor.
A. Lockout Procedure
If a malfunction or need for service occurs, comply with the following
lockout procedure for restricting use of the instrument. All users, upon
observing that the instrument is locked out for servicing or maintenance
shall not attempt to start, energize, or use the equipment.
1. Notify all affected users that servicing or maintenance is required
and that the instrument must be shut down and locked out to
perform the servicing or maintenance.
2. If the machine is operating, shut it down by the normal stopping
procedure (turn off baths and main power or press the EMO switch).
3. Disconnect the power cord from the wall socket and from the
instrument so that the machine is isolated from energy sources
(Fig. XI-A-1).
4. Twist and remove the fuse (Fig. XI-A-2). Place in a locked safe.
5. Contact SciGene Technical Services [[email protected]] or
your local distributor to arrange for service.
Fig. XI-A-1. Disconnect power cord.
Fig. XI-A-2. Remove fuse.
B. Fuse Replacement
The fuse may be replaced by turning off and unplugging the unit, then
gently twisting and removing the old fuse. Replace with a new fuse with
the same electrical rating (250V, 15A for 115V or 250V, 8A for 230V).
˜
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XII.
DECLARATION OF CONFORMITY
Little Dipper Microarray Processing System, Model 650c
SciGene
617 N. Mary Avenue
Sunnyvale, CA 94085 USA
Declares that the above referenced product(s) meets the essential requirements of
the following European Union Directives by using the relevant standards shown
below to indicate compliance.
EMC Directive 2004/108/EC
EN 61326-1
2006
Electrical equipment for measurement, control and
laboratory use to include:
EN
EN
EN
EN
61000-4-2
61000-4-3
61000-4-4
61000-4-6
1995
2006
2004
2007
LVD Directive 2006/95/EC
EN 60204-1
2006
EN 61010-1
2004
2nd Edition
+A1: 1998 +A2: 2001
Electrical equipment of machines
Safety requirements for measurement, control and
laboratory use
Part 1: General requirements
Terry Gill
Sunnyvale, California, USA
Name of Authorized Representative
Place of Issue
Director of Product Manufacturing
Title of Authorized Representative
JuO\ 2010
Date of Issue
Signature of Authorized Representative
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