Download Blue Pippin User Manual - UTA
Transcript
978-744-2244 12 ● www.sagescience.com ● [email protected] Running a Protocol The default screen on the BluePippin is a tabbed format. The Main Tab is the first tab and the default screen. 12.1 Overview The Main Tab is the screen with which users load protocols, run the instrument, and monitor optical signal and electrophoresis. Main Tab Lane Status Panel Graph Display Protocol Status Panel Image Display Control Panel 12.2 Starting a Run 1. Go to the “Main” Tab. 2. Place the calibration fixture on the optical nest. See Section 5 for the detailed information on the calibration procedure. 3. Press “CALIBRATE”. A calibration window will launch. 4. In the “Target I ph, mA” field, enter 0.60 for dye-free cassettes or 0.30 for Midori Green dye cassettes. BluePippin Operations Manual software v 6.00 cassette definition Set 10 12-1