Download 1290 Infinity Flexible Cube - User Manual
Transcript
6 Optimizing Lab Performance with Flexible Cube How to Achieve Lowest Carry Over Times 1 to 4 are the times when the valve switches, to bypass (for time 1) or to mainpass and bypass (for times 2, 3 and 4).The times must be specified in ascending order. You can also switch the times to off. Between the first and second, and second and third valve switches, a rinse is executed using the rinse volumes specified in the Injector Cleaning section. Injection Valve Movements You specify the number of times that the injection valve switches from mainpass to bypass at times 2, 3 and 4 in the field. The maximum value is 2; default is 1. NOTE Time 1 Valve switch directly after sample has been flushed out at high % water might be useful if sample is highly soluble in water. Time 2 Valve switch at high % organic phase to effectively remove sample from injection valve grooves, while avoiding sample retardation on the column. Time 3 Valve switch at low % organic phase to effectively remove organic solvent from injection valve groove. Time 4 Used to repeat valve switch 1, 2 or 3 in the case of extremely sticky sample or solvents. At each time, the valve is switched twice, from mainpass to bypass to mainpass. These additional switches must be taken into account when calculating the number of runs that can be executed until the rotor seal of the injection valve needs to be replaced (EMF). 1290 Infinity Flexible Cube User Manual 93