Download 1290 Infinity Flexible Cube - User Manual

Transcript
6
Optimizing Lab Performance with Flexible Cube
How to Achieve Lowest Carry Over
Times 1 to 4 are the times when the valve switches, to bypass (for time 1)
or to mainpass and bypass (for times 2, 3 and 4).The times must be
specified in ascending order. You can also switch the times to off. Between
the first and second, and second and third valve switches, a rinse is
executed using the rinse volumes specified in the Injector Cleaning
section.
Injection Valve Movements
You specify the number of times that the injection valve switches from
mainpass to bypass at times 2, 3 and 4 in the field. The maximum value
is 2; default is 1.
NOTE
Time 1
Valve switch directly after sample has been flushed out at high % water might be useful
if sample is highly soluble in water.
Time 2
Valve switch at high % organic phase to effectively remove sample from injection valve
grooves, while avoiding sample retardation on the column.
Time 3
Valve switch at low % organic phase to effectively remove organic solvent from injection
valve groove.
Time 4
Used to repeat valve switch 1, 2 or 3 in the case of extremely sticky sample or solvents.
At each time, the valve is switched twice, from mainpass to bypass to mainpass. These
additional switches must be taken into account when calculating the number of runs that
can be executed until the rotor seal of the injection valve needs to be replaced (EMF).
1290 Infinity Flexible Cube User Manual
93