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CircuLex Mouse Adiponectin ELISA Kit User’s Manual For Research Use Only, Not for use in diagnostic procedures On ly! TM the adiponectin trapped in the well in the first Reaction. After washing, the colorimetric substrate for the enzyme is added to all wells and incubated. The color development is terminated by the addition of a stop solution. The intensity of the color is measured at 450 nm. The concentrations of the samples tested are calculated using the absorbance values of the adiponectin standard solutions assayed at the same time. Summary of Procedure Add 100 µL of diluted sample to the wells rp os e Incubate for 1 hour at room temp. Wash the wells Add 100 µL of HRP conjugated anti-Adiponectin antibody Incubate for 1hour at room temp. Wash the wells Pu Add 100 µL of Substrate Reagent Add 100 µL of Stop Solution rR ef er en ce Measure absorbance at 450 nm Fo Cat#: CY-8051 3 Version#: 120807
