1
Download Plasmid DNA Purification
Transcript
Protocol at a glance (Rev. 01) Plasmid DNA Purification 1 Mini Mini NucleoSpin® Plasmid NucleoSpin® Plasmid QuickPure Cultivate and harvest bacteria cells 30 s 11,000 x g 2 3 4 5 6 Cell Lysis 30 s 11,000 x g Buffer A1 250 µl Buffer A1 250 µl Buffer A2 250 µl Buffer A2 250 µl Buffer A3 300 µl Buffer A3 300 µl Clarification of lysate 5 - 10 min 11,000 x g 5 min 11,000 x g load supernatant load supernatant 1 min 11,000 x g 1 min 11,000 x g Buffer A4 600 µl Buffer AQ 450 µl 1 min 11,000 x g 4 min 11,000 x g Bind DNA Wash silica membrane Dry silica membrane Drying is performed during centrifugation of the single washing step 2 min 11,000 x g 9 Elute highly pure RNA 50 µl buffer AE 50 µl buffer AE RT 1 min RT 1 min 1 min 11,000 x g 1 min 11,000 x g
