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Allcian Blue Staining Procedure
e
1.
ue sample should
s
be fo
ormalin-fixed and paraffin-embe
edded already.
The tissu
2.
Staining procedure::
a)
Dep
paraffinize slides and hydrate to distilled wa
ater.
b)
Sta
ain in alcian
n blue soluttion for 30 minutes.
c)
Wash in running tap watter for 2 minutes.
d)
Rinse in distilled water.
e)
Visualize unde
er a light m
microscope and
a
capture
e images fo
or analysis.. Blue
staining indica
ates synthe
esis of prote
eoglycans by
b chondro
ocytes.
Fig
g.5 OriCellTM Rabbit ADSCs
s are different iated to chond
drocytes and are stained w
with Alcian Blue
e.
CRYOPR
RESERVA
ATION OF
O CELL
LS USIN
NG OriCe
ellTM
CRYOPR
RESERVA
ATION MEDIA
M
OriCellTM NCR
R Protein-F
Free Cryoprreservation Medium (C
Cat. No. NC
CPF-10001) is a
use freezing
g medium. Its chemically-define
ed and prottein-free
prrotein-free,, ready-to-u
fo
ormulation has
h been optimized to
o stem cells
s and prima
ary cells, th
hus greatly enhancing
he viability and integrity of these cells by prrotecting th
hem from da
amage durring the
th
on
ne-step free
p
U
Unlike othe
er conventio
onal freezin
ng media, which
w
eze-thaw procedure.
re
equire a slow programmed freeze
e, this prod
duct allows the cells to
o be directly
y frozen at 80
0°C.
Cryopreservation
ge the cultu
ure medium
m with fresh
h growth medium
m
24 h
hours before freezing
N
Note: Chang
g.
1.. Collect ce
ells that are
e in the log
garithmic growth phas
se. Perform
m a cell count to
determin
ne the viable cell dens ity.
2.. Centrifug
ge the cells for 3-5 mi nutes at 25
50 x g and 20°C. Rem
move and discard the
supernattant using a pipette.
IMPI0022A2 RB
BXMDͲ01001
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