Download - Advanced Cell Diagnostics

Contents
Chapter 1. Product Information ........................................................ 6
About this guide ............................................................................................ 6
Product description ........................................................................................ 6
Background............................................................................................... 6
Overview .................................................................................................. 6
Kit contents and storage ................................................................................. 7
RNAscope® VS Probes ............................................................................... 7
RNAscope® Control Slides .......................................................................... 7
RNAscope® VS Reagents ............................................................................ 8
Required materials from Ventana™ Medical Systems ........................................ 9
Equipment and buffers ............................................................................. 10
User-supplied materials ............................................................................ 10
Chapter 2. Before You Begin ......................................................... 11
Important procedural guidelines ................................................................... 11
Chapter 3. Prepare and Pretreat Samples ........................................ 12
Prepare FFPE sections .................................................................................. 12
Materials required ................................................................................... 12
Fix the sample ......................................................................................... 12
Dehydrate, embed, and cut the sample ..................................................... 12
Chapter 4. Semi-automated RNAscope® VS Assay ........................... 14
Workflow ................................................................................................... 14
Prepare the materials ................................................................................... 15
Materials required ................................................................................... 15
Prepare the instrument ............................................................................. 15
Prepare instrument reagents ..................................................................... 15
Prepare detergent .................................................................................... 16
Prepare deparaffinization reagents ........................................................... 16
Prepare mounting reagents....................................................................... 16
Prepare 1X Pretreat 2 .............................................................................. 16
Create an instrument protocol ................................................................... 16
Print the labels ......................................................................................... 19
Manually pretreat the samples ...................................................................... 20
Materials required ................................................................................... 20
Bake the slides ......................................................................................... 20
Deparaffinize FFPE sections ...................................................................... 20
RNAscope® VS Assay for the Discovery® XT User Manual - Red
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