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Directionsfor Usefor the Fibrosystem@- The Total Systemfor CoagulationTesting
i
[:ibrometer@ PrecisionCoagulation Timer
C a t a l o gN u m b e r6 0 4 1 5
N H H T CR e f . N o . H 8 2 9 2 - 2 1 3 4 1 3
f h e r m a l P r e pB l o c k
C a t a l o gN u m t r r 6 0 4 1 9
Nl{RlC Ref.No. }'tBZ9Z"Z135'13
,rlrrtorrrutic
E lectric Pipette
CatalogNumber 60420
NH R tC Ref" No. H 8292.213613
I
TRADEMARKS
.
The followingaretrademarks
of .Becton,DickinsonandConrpany- BBL, BART, Fibrometer,
FibroPlastin,
FibroSystem,
FibroTip,FibroTrol,FibroTubeand B-D.
ABB[g
d r v r s r o no l B e c t o n , D r c k r n s o na n d C o m p a n y @
C c r i ; k e y s v r l l eM, D 2 1 0 3 0 U S A
CONTENTS
"
Precautions
,iv
Section I
FibroSystemo- The Total System for CoagulationTesting
1
Section 2
T " h eF i b r o m e t e r @P r e c i s i o nC o a g u l a t i o nT i m e r
l
Section 3
T h e A u t o r n a t i cP i p e t t e
23
.
Section 4
'ilre
T hermal Prep Block
33
:
Section5
StandardOperatingProcedure: ProthrombinTime Test
'.iection
6
PreventiveMaintenanceProcedurefor 60420 & 60422
Automatic Pipettes
43
I
47
I
kction 7
Use of FibroTubeo DisposablePlasticCoagulationCups with the
PrecisionCoagulationTimer
Fibrometer@
....
53
*ction I
1-roubleShooting and Repair
TroubleShooting
RepairProcedures
ReplacementPartsLists
Wiring List - Fibrometer@
Wiring Diagram- Fibrometer@
SchematicDiagram- Fibrometer@
......
61
72
77
81
82
84
Section 9
W a r r a n t ya n d R e p a i rP o l i c y
87
Bibliography
91
t
.
iii
a
'
PRECAUTIONS
R e a dt h i s m a n u a lb e f o r et e s t i n gc o n t r o l so r c l i n i c a l ' s a m p l ewsi t h F i b r o S y s t e m @ e q u i p m e n t .
T h i s m a n u a lh a sb e e nd e s i g n e du t i l i z i n ga s t e p - b y - s t efpo r m a t . l t i s m e a n tt o p r o v i d e
all the
i n f o r n r a t i o nt h a t i s n e e d e dt o r u n a c c u r a t et e s t sf o r f i b r i n f o r m a t i o n .N o t o n l y w i l l t h i s m a n u a l s e r v ea s a h a n d y r. e f e r e n c eb, u t a l s oa s a n i m p o r t a n tt r a i n i n gg u i d ef o r n e w p e r s o n n e l .
.-
FOR YOU R PROTECTION, the Fibrometer@
P r e c i s i o nC o a g u l a t i o nT i m e r a n d t h e T h e r m a l
P r e pB l o c k a r e e q u i p p e dw i t h a t h r e e - w i r eg r o u n d i n gp l u g . U s e dp r o p e r l y ,t h i s g r o u n d i n g
plug will protect you from shock hazards.Should a two-prong receptaclebe encountered,it
ntust be replacedwith a properly groundedthree-wirereceptaclein accordancewith the
National ElectricalCode and local codesand ordinances.This work shouldbe done by a
q u a li f i e d e l e c t r i c i a n .
DO NOT,underany circumstances,
cut or removethe grounding
prongfrom the plug.
UNPLUGthe powercordbeforecleaning
performing
or
any of the proceduresdescribed
in
tlre sectionentitled,"TroubleShooting".
DO NOT performany servicing
exceptfor thoseitemsspecifically
statedanddescribed
in'
this manual.Adviceon specific
problems
technical
canbe obtainedby contacting
the TechnicalServlces
Depar-tment
at BBL, PostOfficeBox 243,Cockeysville,
Maryland21030or
telephone
toll-free,(800)638-8663;
in Maryland
call(301)666-0100coltect.
DO NOT openany instrument
your
coverunderany circumstances.
To do so may invalidate
warranty.
DO NOT removelabelsor warningtagsfrom this equipment.
eachproduct.
only asdirectedin the packageinsertaccompanying
UseFibrosystemReagents
iv
S E C T I O NO N E
F I B R o S Y S T E M-@
T H E T o T A L S Y S T E MF O RC O A G U L A T I OTNE S T I N G
FibroSystem,
the registered
trademarkof Becton,DickinsonandCompsnV,
isa total system
containing
all the equipment,
reagents
anddisposable
supplies
needed
for accurate
coagulaconeeptprovidesa reliablemethodfor performingany labor. tion testing.The FibroSystem
atory testwhichutilizesa f ibrinclot asthe endpoint. lts rangeof operationincludes
diag
rrusticevaluation,
monrtoringanticoagulation
therapy,andmeasuring
the effectof heparin
artdprotaminesulfateon wholebloodduringopenheartsurgery,renaldialysis,
etc.
EO UI P ME N T
'
'1.
Timer
Fibrometer@Precision
Coagulation
-l
Precision
is the Fibrom€ter
he majorcomponentof the FibroSystem
Coagulation
Timer.
The instrument
isdesigned
will be calledthe Fibrometer.)
{ln thismanual,thiscomponent
1nautomatically
detecrfibrinclot formation.
?. ThermalPrepBlock
the Fibrometer.This unit is a precisedevicefor preThe ThermalPrepBlockcomplements
Temperature
equilibriumis maintained
reagents
andplasmato testingtemperature.
bvarming
1d
0 d e e p w e l l s . T h e s l r a l l o w w e l lascwciol lm m o d a t e
at37.2t0.5Cin20shallowwellsan
to hold standard12 X75
FibroTubs@Coagulation
Cups.The deepwellshavebeendesigned
mrn test tubes.
3. AutomaticPipette
is the AutomaticPipette,which is capableof
The third componentin the FibroSystem
testing.Onceplugged
the 0.1 ml and0.2 ml volumesnormallyusedin coagulation
dispensing
into the Fibrometsr,the AutomaticPipettewill servea doublefunction.This hand-held
pipetteliquids,and it willautomaticallyinitiatethe Fibro'
pipettinggunwill accurately
meterclot detectingandtimingmechanisms.
D I S P O S A B LSEU P P L I E S
,
reactioncup for usewith the Fibromsterand the ThermalPrepBlock is
The reqommended
Cup.Threefactorsmakethesecupsan important
Coagulation
the FibroTubeDisposable
to en$lre
component.First,they aremadeto carefullycontrolleddimensions
FibroSystem
they are
Second,
well.
reaction
Fibrometor
in
the
proper
is
maintained
liquid
level
that the
to
block
from
heating
proper
transfer
heat
that atlowthe
madeof specialmaterials
fiquid.
which eliminateerrorsdueto contamirlation.
Finally,thesecupsareonetimedisposables
pipettetip for usewith the AutomaticPipetteis the FibroTiorMDisposThe recommended
PipetteTipsshouldalwaysbe usedwhenpipetting
atrlePipetteTip. FibroTipDisposable
tips areeasyto useand
clean,disposable
with the AutomaticPipette.Thesechemically
e|iminatethehazardsassociatedwithmouthJripetting
RN
EAGENTS
BBL@COAGULAT|O
i
Whenthesereagents
aspart of the FibroSystem.
a broadlineof reagents
BBL manufactures
performthe mostmoderncoagulation
areusedasdirected,any laboratorycanconveniently
for thesetestsareprovidedin the packageinsert
tests.Gompleteand detailedprocedures
personnel
shouldreadthe insertbeforeusingthe
eachproduct. Atl
which accompanigs
is shownin the charton the Rextpage.
product.A completelist of BBL coagulationreagents
t
ti.
i
I
BBL@COAGULATIONREAGENTS
.
40775,76t FibroPlastin@
$/ith Calcium
RabbitBrainThromboplastin
408.21,22
t FibroPlastin@
ll Liquid RabbitBrainThromboplastin
with Calcium
40777
ControlPlasrna
t FibroTrol@
CitrateNormalCoagulation
40778
ControlPlasma
t FibroTrol@
OxalateNormalCoagulation
40779,80t FibroLet@
ActivatedPlateletFactorieagen.
.
40802
ControlPlasrnaKit
t FibroTrol@1O20CitratedAbnormalRangeCoagulation
40805
ControlPlasmaKit
t Fibroirol@10-20OxalatedAbnormalRangeCoagulation
40813
t FibroFacrMCorrectionReagentKit
40823
BARTTMReagent
40631
t PlateletFactorReagent
40636
FreePlasma,BovineSoorce
t Prothrombin-
40637
SodiumOxalate,0.1Molar
40662
CalciumChloride,0. )32 Molar
40638
CalciumChloride,0.02Molar
40639
CalciumChloride,0.025molar
40640
Generation
GenerationKir (for performingthe Thromboplastin
t Thromboplastin
Time Test)
Owren ProthrombinTime Roagent
4Cf.41,42t* ThromboTest@
* Trademark of Nyegnard& Co., A/S, Oslo, Norway
t Refrigerate.Store a!2 to 8C'
O
a
a
a^'-.4
a
o o o
o o o
0
o
o
o
I lk:uglI
ilffi|
t
t
n
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r
it
t t
I
II
t
t
u
o o
o o
00
o o
oo
o o
l
I
\-
/
Sirrqle
F ibrorneter
t
Pairof
F i l :ro m e te r
Urrrts
Fibronreterand
T h e rm a lPrep
B l o c kU n i t
P ai rof Fi brometer
U n i t sw i t h T h e r r n a l
P r e pB t o c kU n i t
P ai rof Fi brometerU ni tsw i th
P a i ro f T h e r m aP
l r e pB l o c kU n i t s
F i g u r e1 -1 The ModularConcept
I ' H E M O D U L A RC O N C E P T
Due to the smallarnountof electricityusedby the FibroSystemcomponentsand their
specialdesignfeatures,modular conrbinationsare possible.Built into oppositesidesof both
plugsand
the Fibrometerand the Thermal Prep Block are specialpolarizedthree-pronged
plugged
into
any
be
other
Fibrocan
these
machines
of
that
any
receptacles.
This means
power
from
one
three-wire
line
meter or Thermal Prep Block. The first machinewill obtain
At the sametime, line power will passon to the second
cord pluggedinto a wall receptacle.
machinenow pluggedinto the first. lf a third machinewere to be pluggedinto the second,it
too would receivepower. This modular conceptwill not overloada circuit becauseof the
small amount of electricityactuallybeingusedby eachmachine.For example,a system
composedol two FibrometerTimers.andtwo Thermal Prep Blockswould usea maximum
current of 3.0 amperes.
The modular concept is expandedstill further by pluggingthe Automatic Pipettecord directly into the connectoron the front of the Fibrometer.
E x a m p l e so f m o d u l a rc o m b i n a t i o n sa r e s h o w ni n F i g u r e1 - 1 .
.
sEcTtoNTWO
T H E F T B R o M E T E RPO
REcISIoN
c o A G U L A T I o NT I M E R
T H E F I B R O M E T E@
HP R E C I S I OCNO A G U L A T I OTNI M E R
usE
I
T i m e r i s a n e l e c t r o - m e c h a n i cianls t r u m e n t h a t ' w i l l
T h e F i b r o m e t e rP r e c i s i o nC o a g u l a t i o n
cieterminethe presenceof a fibrin clot. The Fibrometeris both a clot sensingdeviceand a
t i m e r . E a c hu n i t a l s oc o n t a i n s c o n s t a ntte m p e r a t u r ew a r m i n ga n d r e a c t i o nw e l l s .T h i s ' b a s i c
clesignallowsthe Fibrometerto be usedas the primary pieceof equipmentin most of the
c o a g u l a t i o nd e t e r m i n a t i o nns o w i n c o m m o n u s e .
l
i
I
P R IN C I P L E S
OFOPERATION
:
l-listorically,those wisfiingto perform coagulationdeterminationsutilizedthe wire loop
marrualmethod for measuringthe critical clotting end point. The Fibrometerwas designed
t o r n e c h a n i c a l lrye p l i c a t et h i s p r o v e nm a n u a lm e t h o d ( 1 , 2 ) . N o w a f t e r m o r e t h a n t e n y e a r s
of succesful useand millions of field determinations,the Fibrometeris a time'tested'device.
The Fibrometeris a ruggedunit which ensuresthe quality performancedemandedbY modern laboratorystandards.When usedfor testsendingwith fibrin formation, this machineis
capableof sustainedaccuracy,consistencyand reproducibility.
are detailedon the following page.
and specifications
The performancecharacteristics
'
.-
TIMER
PRECISION
COAGULATION
THE FIBROMETERO
andSpecifications
Characteristics
Performance
'
Temperature 37.2,0.5 c
Heating
DetectorCycleTime
0.5 sec.
Machine
Resolution
I 0,5sec.
TimingRange
0.0to 999.9sec..
StandardDeviation'
0.3 sec.for timesunder25.0sec.
0.6 sec,for timesover25.0sec.
DilutionCurves,
usingthe Fibromotorhavetha same
Curvesestablished
obtainedby visualmethod&
as
thoso
characteristics
TestVolumes
0.3ml and0.4ml
PatientSamplesize0'lmlor0.2mlofpatient'splasna,wholeblood,or
serum
occasionallY
LiquidHeating
- Times
fluidr:5 minutes
Refrigerated
liquids:3 minutes
Roomtemperature
andWeights Height(probeupl ' 178mm {7"}
Dimensions
Width- 102mm (4"!
Depth.-184mm (7.25"1
NetWeight- 3 Kg (7 lbs.)
Weight- 4.8 Kg (10.5lbs.)
Shipping
Time
Warm-Up
Machine
10minutes
Approximately
ElectricalspecificationsllTvolts,OQttz'Q'Qamps(CatalogNo'6O415)
No' 6M161
117volts,50 Hz,0.6amps(Catalog
by daraobtalneduring$e ouick one-tti-grtschniquolnd th. ws'?strsgn'll
G"*-*..
(3'4)'
or ".,pportsd
O"len'' te:t for Prothrombinsnd Proconverdn
-"airi."iion "ru
10
a
.
C O M P O NE N T P A N T S
The Probe Unit
l
l
The clot detection arm of the Filrometer
is the probe unit. Two probe units are
availableand may be used interchangeably
on any Fibrometer. Each Fibrometer
comeswith one standardprobe, BBL€'}
C a t a l o gN o . 6 0 4 1 7 .T h i s p r o b e i s d e s i g n e d
to be usedwith sampleshavinga volume
o f 0 . 3 m l . F o r s a m p l e st h a t r e q u i r e0 . 4 m l ,
the usermay order a specialprobe, BBL
'
C a t a l o gN o . 6 0 4 1 8 .
1
4- MOVING
E LE C TR ODE
T h e p r o b e u n i t i s m a c l eo f a c y l i n d r i c a lb o d y ,
two sensoryelectrodes,and a heavy wire
calledthe probe foot, which extendsfrom the
baseof the assembly.Thesestructuresmay be
s e e ni n F i g u r e2 - 1 .
STATIOI{ARY
E LE C TR O DE
The entire probe unit fits smoothly into the
probe sleevewith the electrodespositioned
d i r e c t f yo v e r t h e r e a c t i o nw e l l ( F i g u r e2 - 2 1 .
Betweentests,the probe is placed in the UP
position.This allowsfor easycleaning,insertion of FibroTube@cups and pipetting.
For detailsconcerningthe probe drop and
electrodemovement,seeCOMPONENT
PARTPRINCIPLES
OF OPERATION.
.,
,
Figure2-1.TheProbeUnit
The Electrodes
steel,arethe only parts9f the Fibro'
madefrom #304 stainless
Two durablemetalelectrodes,
is a movinganda statignaryelec'
There
mixture.
reaction
meterthat makecontactwith the
in an elliptieal
by sweeping
rnixture
trode.The movingelectrodecyclesthroughthe reaction
will pick up the initlalfibrin thread(monopattern.A smallhook on the tip of thiselectrode
for creatingan electricpotentialbetweenthe
mer),The stationaryelectrodeis responsible
"sensing"position,the stations{;Blectrode
or
DOWN
Whentlre probeis in the
etectrodes.
remainsin the reactionmixture.
The,ProbeFoot
The,heavywire extendingfrom the baseof the probeis the probefoot. The movingelectrode
is an extensionof this probefoot. A cam raisesand lowersthe probefoot, thus movingthe
electrode.The probefoot alsois the contactpoint betweenthe movingelectrodeand the de
tectioncircuitbf the Fibromstor.
1l
P R O B EA R M
M O V I N GE L E C T R O D E
S TA TION A R YE LE C TR OD E
R E A C T I O NW E L L
W A R MIN GW E LLS{ 6I
INDICATOR LAIVIP
READOUTRESETBUTTON
DIGITALREADOUT
I ND E XI N G
DOT
W
POWER
CONNECTION
TIMERBAR- .(
O N - O F FS W I T C H
PL U G .INJ A C K 6 U T L E T ' ---<
( F O RA L T E R N A T EO P E B A T I O NW I T H
W I T H A U T O M A T I CP I P E T T E I
Heatin g B l o c k
'
-
,,: .tig'ru 2'2'ihe" Fibrometor
^:
'' " ': :
" '":
"'^:'
to heatand hold liquidsat37.2t 0.5 C. The
The c,hrome-plated
heatingblock is designed
longthermalconstantto gnsurethat the proper
a
has
which
of
metal
is
block itse,'i composed
prewarming
wellsanda reactionwell arebuilt into the
will be held.Six
testingtemperature
block.
12
The DetectionCircuit
,.
The functionof the detectioncircuitdsto sense
the circuitformed(completed)
whena fiprin i
$trandis detectedby the movingelectrode.Whena f ibrin strandis detecied,the Fibromeier i
will stoptimingandceasemechanical
action.lf no strandof fibrin ispresent,
thereis no flow
of currentthroughthe detectioncircuit.However,whena fibrin strandisdetected,current
f lowsthroughthe detectioncircuitanddeenergizes
a relay.The relaycontactsopenand stop
both the timingandthe mechanical
operation.
The detectioncircuitmay be classified
asa
bistableor flipf lop transistorized
,
circuit.
,,
The iylotorand CountingUnit
,;
I
The Fibrometercontainsa synchronous
clockmotorto run the countingunit andto prqpuce;
electrodemovement.The precisespeedof the thismotor is regulatedby the frequencyqf the
powerline.The motor hastwo shafts.Oneshaftmovesthe camat 60 rpm.The othershaft
drivesa pulsedcountingunit at 600 rpm.Thisarrangement
enables
the countingsystemio
' havea resolutionof 0.1 second.
,
r
Readout Reset Button
I
This small red button resetsthe counter to zero when depressedfirmly for at leastone second.
Indicator Lamp
:
The function of the indicator lamp is to let the user Know when the machinehas reachedoperatingtemperature.This light will come ON only when operatingtemperaturehas beenobtained. lt will stay ON as long as the machineremainsat this temperature.
I
DO NOT performFibrometertestsunlessthe indicatorlight is ON.
l'
i
Safety
l
a
The Fibrometeris certifiedfor safetyby the LosAngelesCountyTestingLaboratoryand is
(CSA).
listedby the Canadian
Standards
Association
:
.
i
I
coMPoNENTPARTpRtNCtpLESOFOPERATION
;,
'
1
This sectionshouldbe readand understoodcompletelybeforebeginningroutineuseof the
FibrometeJ.
The faclorsouttinedandexplainedbelow'arevital to accuracy.
i
I
HeatingSystem
- -,r,
:t::
.
i
The heatingblock cctntains
two mercurythermostats
andh l;rgate1-that
will bring liquidsin the
weffs to 31.2 t 0.5 C. The operationof thed iherniostatsis automatic.The temperatutecant
not be adjustedby the operator.
i
This unit will bring liquidsat room ternporatuF€,to,approximately
35 C in aboutten minutes.
takesover.This
ther.rnostat
At this point, the indicator:
light corneson arld-the",fine"-"control
sensitive,
tfrermostatwill reactto a 0,'1Q tempgr-aty,rp
changeinsidethe block and maiptaina
temperature
of 37.2 t 0.5 C.
i
t-
13
I
i
1
I
L{
i:
'6.
i!
t{:
b
MechanicalAction - Probe Drop
The Fibrometercan be activatedmanuallyby depressing
the TIMER bar or automatically
when the Automatic Pipettehasdeliveredits last bit of liquid into the reactioncup.There is
a built-in delay of 0.5 to 1.8 secondsbeforethe probe drops into the reactionwell. The delay
allowsfor the removalof the pipette tip from the reactioncup. This delay will not affect the
test results.
After the built-in delay,the sensingprobe assemblydrops into the reactionwell. Beforethe
testingof a sample,the probe is placedin its rbst position abovethe reactionwell. In this
position,the probe restsupon the probe support lever.This leveris mechanicallyconnected
to a solenoid.When the delay circuit energizes
the solenoid,the leversnapsback insidethe
probe sleeveto deprivethe probe of its support and causesit to drop. The drop placesthe
electrodesinto the proper position insidea FibroTubecup in the reactionwell.
MechanicalAction - ElectrodeMovement
Movement of the sweepingelectrodeis suppliedby a segmentedcam that rotatesat 60 rpm
and raisesthe probe foot every 0.5 second.This sweepsthe electrodethrough the reaction
mixture every one-halfsecond.The electrodemovesin the desiredellipticalpattern through
the reactionmixture. This can be viewedas a four step process:
1) The moving electrodesweepsthrough the reaction mixture.
2l Theelectrode liftscompletely outof the reactionmixtureto a point 1.27 mm above
the liquid level.(NOTE.:This is why the FibroTube cup is of such importafice iri the
FibroSystem.Useof thesecups en$rresproper liquid level.)
3) The electrodebecomeselectricallyactive.
4) The electrodeis returned to the liquid level and snreepsthrough the reaction mixtule
to completean ellipticalpattern.
Clot Detection
As the initialf ibrin strandformsin the reactionmixture,it is pickedup on the next sweep
of the movingelectrode(seeFigure2-31.Whenthis electrodeis liftedto a point1.27mm above
the levelof the liquidin the FibroTubecup,a portionof the f ibrinstrandwill remainin the
in the liquidreactionmixture,a
remaining
electrode
liquid.At this point thereis a stationary
the
movingelectrodesituated1.27mm abovethe liquidlevelanda fibrin strandconnecting
mixture.
to the reaction
movingelectrode
is liftedto its positionabovethe liquidlevel,it becomeselec'
Whenthe movingelectlode
tricallyactive.Rtfipriilstrandwi!l completethe cirbuit.Cu?rentflowsfrom the stationary
The
through:theliQuidandthroughthe fibrln strandto the movingelectrode.
electrode,
detection
and
the
cam
to
electrode
circuitis completedascurrentf lowsf rom the moving
the Fibrometerand its timingdeyiceto stop'
circuit.Thecompletionof this c.ircuitqauses
This isiuhy the Fibrometeris known asa f ibr:inswitch.detectiondevice.
FibroTubecupsenNoteonceagain,the levelof the liquid''inthe reactisnwell is'critical.
surethat the properliquidlevelcanbe maintairnd.
14
MOVING
ELECTRODE
UPPOSITION
ST A T IO N AR Y
EL EC T R OD E
MOVING
E L EC T R OD E
DOWN
POS T T tO N
FIBROTUBEO
.i
CUP IN
i
REACTION
WELL
FLUID
LEVEL
l
f .o...
PATHOF
MOVING
I
,,
ELECTRODq
r-gfl
$
S.'J
I
l
E
E
: ==cE.
!t
--a!=--
E
i-'! :
x t
f
o1
Or
oj
o
-or
.
r
F
r
.:
-
a
-
-::_
_ /
-^/
.r
Figirre 2-3.Clot Detection
TNSTALLAT|ON
EquipmentModelsand PowerRatings
BBL 60415
*BBL 60416
Fibrometer
Fibrometer'
117volts,60H2,0.6amps
117 volts,5OHz, 0.6 amps
l
l
A drgppingtransformer,BBL CatalogNo. 60!76; is available
for loweringthe 22Qvolt power
sourceof manycountriesto 110volts,thusenabling
the Userto employeithera 50 or 60 Hz
Fibrometer.The frequency(Hz) ratingof the Fibrometermusf agreewith the localline frequeniy. Up to t?voFibrom€tersand one ThermalPrepBlock may beconnectedtb a single
transformer.
I
. . ,
. .
:
r i , : . . :
_ . ,
a
'
I
.
This mrchine is intendedfor usein oountrieswlth 50 cycle (Heftz) alternatingcurrent.
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LaboratoryRequirements
The Fibrometeroperatesor a standard117-voltA.c. power
sourcewhich must meetthe
NationalElectricalManufatturers
Association
standardof gg.s to r2g.7volts.The outlet
must be a standardlaboratorvthreewire groundedreceptacle.
other powersourcesor outreut
maydamage
the machine.
The workingenvironment
shouldbe ascleananddust-free
as
possible,
with an ambienttemperature
between18 and32 c (64.4to gg.6 F).
Procedures
The followingprocedures
aredesigned
to alto* yo, to setup andtestyour Fibromstor,
stepby-step'lt is recommended
that you testthis unit independently
of the ThermalprepBlock
andthe AutomaticPipette.lf you planto connecttwo Fibrometer
timersto a singlepower
source,eachuriit shouldbe testedindependently.
lf any problemsoccurasyou testyour new
Fibrometer,
please
caltthe BBL Hot Line,toll-free:(g00i 63g-g663;in Maryland (301)
call
666-0100 collect.
l. Beginby placingthe Fibrometerbox
w i t h t h e w o r d s ", O P E NT H I SE N D , '
in the UP position.
Takeout the
packingmaterialandremovethe
machine.
Includedin thisoneshipping
cartonare:
A. One Fibrometer
B. Onelinecord
C. One0.3 ml probe
D. 400 FibroTubecups
Visuallyinspectall itemsfor any indication
of shippingdamage.
WARNING: The 0.3 ml probeis packedin the DOWNposition;i.e.,in the probesleeve.
DO NOT turn the Fibrometeroverwith the probestill in the machine.Thereis no mechan.
ismto keepthe probefrom fallingout of the well and awayfrom the base.
2. Placethe machineon a levelbenchtop that is freefrom vibration.At this time, it
would be wiseto decideif additionalspaceis neededfor other modulesof the FibroSYstem' ''
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.-,
or
on thesamebenchwith a rQtarycentrifugo,
CAUTION:DO NOTptacethe Fibromstsr
mercury
the mercurycolumnin the sensitive
mAyseparate
Mechanical
shaker.
shaking
temperature.
thermostat
andimpairtheabilityof theunit to holdthecorrectoperating
eucha probfemoccur,seetheSectiontitled,"TroubleShooting."
Shoulcl
16
3. Plugthe groundedthreewireline
Cordonto the prongsfound on the
left sideof the Fibrometer.
NEVER
plugthe linecordinto th8 wall
receptacle
first and then attemptto
connectto the machine.lnsure
properalignmentby matchingthe
red dots found abovethe prongson
the Fibromotorand on the linecord.
4. Plugthe line cord into a standardlaboratorythreewiregroundedreceptacle.
DO NOT
useany other receptacle
or modify the linecord to fit any othertypo of rec;ptacle.
vvAHNING:AN UNGROUNDEDRECEPTACLE
CAN CONSTITUTE
A SHOCI(l{AZAnD
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FOR THE OPERATORI
Flemember
to reducethe variables
in the testingof all equipment.
At this point,testonly one
Fibrometer.After completionof this test,proceedto testadditionalFibrometerTimers,
ThermalPrepBlocksandAutomaticPipettes.
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OperatingInstructions
The followinginstructionsarefor a test run of the Fibrometer.The test beingutiiizedis the
timesto be certainthe Fibromoteris
ProthrombinTime Test.Be glre to run this test several
properlydetectingandtimingfibrin strandformation.lf a problemis notedin thesetestruns,
please
toll-free:(800)63&8663;in Marylandcall (3F1) 666Services,
call BBL Technical
0100collect.
1;' Put the Fibrometerpowerswitch
,' in the ON position(switchup).
:
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at
Waitapproximatelyten minutes
for the machineto reachProper
operating temperature.
.f he indi' '
and re'
tiri
come
ligfit
witi
ritor'
mainon aslongasthe machineis
at the correctoperatingtemPera
ture. t
3. Raisethe probearm to the UP
(rest)position.Whenthe machine
is not in use,the prgbeshouldbe
left in the DOWNpositionto pro
tect the electrodesfrom accidental
damage.Pleasenote that the probe
cannotbe forceddown into the
probesleeve.One must srruitch
on
the machineanddepres the Timer
Barto drop the probeinto the
DOWNposition.
4. The Fibromotoris an independent
unit. lt is capable
of prewarming
reagents
and samples.
Placesix
FibroTubecupsinto the warming
wells.Usinga felt tip marker,label
one
onecup "Control Plasma",
cup "PatientPlasma"andthe re
mainingfour cups"Reagent".
0.3 ml of
5. Pipetteapproximately
BBL
Plasma,
Control
FibroTrot@
CatalogNo. 40777, or BBLCatalog
No. 40778,into the controlcuP
0.3
approximatelY
and,if desired,
plasma
the
into
patient
ml of
patienttube.
Re
6. Pipette0.2 ml FibroPlastin@
No.40775,or
agent,BBL Catalog
BBL CatalogNo. 40821into each
of the four remaining"FibroTube
NOTE: The useof FibroPlastlnReagent
This is the reagentused
is iecorrlmended.
by BBL to calibrateall FibromstsrTimers at the frctory.
18
7. Allow threeminutesfor room temperatureliquidsto cometo operatingteniperatlrre
in
'
for refrigerated
liquids. j
the warmingwells.Fiveminuteswill be necessary
into the center(reaction)well.
8. Placeone FibroTubecrtpwith FibroPlastin
'.
9. Pipette0.1 ml of FibroTrolControl Plasmainto the cup and simultaneouslypressthe Timer Bar.
NOTE: With the AutomaticPipette,thereis no needto pres the Timer Bar.Wlienthe Automatic Pipetteswitchis ON, the Fibromoteractionis begunautomaticallyasthe:lastbit of
liquidis expelledfrom the pipette.
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10. The probeshoulddrop and the
movingelectrodeshouldbeginits
patternthrough
ellipticalsweeping
the reactionmixture.A clicking
soundwill be heard.This is normal.
11. The digitalreadoutandelectrode
actionwill stopwhena fibrin
strandis detected.Readand record
the time requiredfor fibrin strand
detection.lf the Fibromebr has
properlydetectedthe formation of
the first fibrin strand,the time :,
shownon the digitalreadoutshould
be from elevento thirteenseconds'
19
12. Lift the probe arm up to the rest
position.The electrodeshould be
wiped dry with a linl-free laboratory gradeabsorbenttissueor
b i b u l o u sp a p e r .
NOTE: This tissueor paperMUSTbe lint-free.The electrodemust be freeof iint and all
other forrnsof contaminationfor properf ibrin stranddetection.A distilledwaterwashdilf an acidwashisdesired,use
rectedfrom a washbottle may be usedto cleanthe electrodes.
phosphoricacidfollowedby a distilledwaterrinse.The useof otheracidsmay pit the
l'.OYo
electrode.
'13. Setthe timer to zeroby firmly
depressing
the red resetbutton
for at leastone second.
14. Repeatthe ProthrombinTimeTest,Steps7 through 13.
PipettingHints
of which ryr
properand consistentpipettingis necessary
for sustainedaccuracy.Rogardless
proceduro:
the
folloYing
pipetting,
adopt
standard
or
tem you use,the AutomaticPipette
the cup that is closestto the centeroi tt t Flbrome$t,
,' , Ol Restthe pipetteon the lip of
oppositesidewall or the reactionmi,xturei
the
touch
DO NOT let the tip
insertthe pipertetip at least1/4 of the way intoitheFfbroTubo
B) Whendispensing,
cl
cup.
cup.
Aimthetip-attheuug,[bottomof theFibroTube
D)
Ejectthe materialsmoothlyandwithout splashing.
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an unoqual
DO NOT allow the liquid beingpipettedto run down the sidewall.This {vill ceiss
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shortend point.
liquid leveland resultin an erroneous,
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coNTAMiNATtoN:Thesamepipetteor Fibrolibgn beugedfor
AVord prpETTING
A differentpipetteor FibtoTipMUSTbeused
or sample.
pipettingeachaliquotof a reagent
for.eachplasmasPecimen'
20
OPERATING
H I N T SA N D P R E C A U T I O N S
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The FibrometerPrecision
Coagglation
Timeris designed
for In Vitro Diagnostic
Use;all
directionsshouldbe readandfollowedcarefully.
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WARNING: Everyhumanblood
samplerepresents
a potentialHEPATIT|SDANGER. l
Observeproperprecautions
againstthis infectioushazardwheneverhAndlirlg
plasma,serumor wholebloodspecimens.
(b)
I
Electrodes
Thestationaryand movingelectrodes
shouldbe alignedin a verticalposition,paraitrltojon.
another.Eventhoughspacing
is not critical,grossdistortionsshouldbe corrected.lfthel
electrodesbecome bent, carefully realignthem so that they are both vertical and farallef to
one another.
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As noted,lint contaminationof the electrodes
will resultin erroneous,
shortcoagrtlatiorr
times.Uselint-freelaboratory-grade
absorbent
tissueor bibulouspaper.
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Mechanical
Operation
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The Timer bar must be pressed
firmly to ensureproperprobeactivation.The timqr reset
button mustbe depressed
firmly for at leastone secondto resetthe digitalmechqhism.
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Failureto do so maycause
slipping
or jumpingof the timerwheels.
Wiresand Outlets
;
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}
Separate
modularunitswith care.Electricalsafetyprocedures
alwaysrecommen{that the
powercord be unplugged
or disconnecting
modularunits.
beforeconnecting
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When machinesare in a modulararrangement,keepthem on a levelsurfaceand hjesureto
avoid twisting the connectors.
Cups
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FibroTubecupsaresingleusedisposables
manufactured
by BBL. The liquid leveiin thb reactionwell is criticalto the operationof the Fibromstor.
ReagentOuality
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for the besttest results.However,any'properlypre
FibroSystemReagents
are recommended
ducedand correctlystandardized
reagentand controlplasmawill work with the$Fibromotar.
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ReqgentVolume
.
-
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The Fibrometorcomeswith a probecalibratedfor usewith exactly0.3 ml of li{uid. A 0.4
ml probeis availablefor usein testsrequiringthis volume.To ensureproperliqriid le{els,it
da
is recommended
that FibroTubecupsbe utilized for all tests.To enzurecorrecQvolume
'n
t,
fivery,usethe Automatic PipettetBBL'CatalogNo.6O420.
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Temperature$larnings
,
T[iomboplastin reagentg
rhould neverbe left at^g7.2C for morethan four hotfs. Plisma
:
for morethan twgnty minutes.
shouldnot be left at this temperature
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M A IN T E N A N C E
The Fibronreter
is designed
with userconvenience
in mind.Thereis a minimumof maintenancerequired.
The timingmechanism
is factory-sealed
and neverrequireslubrication.
There
areno zerocalibrations,
thresholdsettings,
temperature
controls,or day-to-dayadjustments
necessary
with the Fibrometer.All that is requiredof the userarea few simpleprocedures,
asoutlinedbelow.
ElectrodeCare
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Proteinbuild-upcan significantlyaltertesttimes.Removeall dust,debrisandtestingcontam.
inationfrom the electrodes.
Cleanelectrodes
of foreignmaterialby wipingthe electrodes
with lint-freetissueor paper,or rinsewith distilledwaterby usinga washbottle.
Warmingand ReactionWells
Periodically,
removeaccumulated
dustand debriswith a slightlydampened
cotton nnrab.Dirt
or debriswill raisethe levelof the FibroTubecup.This in turn will raisethe levelof the liquid
in the reactionwell anddrastically
alterthe coagulation
time measurements.
Checkthe well
eachday beforeuse.
ProbeGolumn
No portion of the probeassembly
shouldeverbe lubricated.The materialsand mechanism
of
probe
post
probe
this
aredesigned
to operatedry. After approximately1,000
drops,the
hole
if
andthe probecolumnmustbe wipedwith a lintlesssoft tissue.Thisshouldbe done,-even
in the posthole,it must
isvisible.
no dirt accumulation
Shoulddirt be allowedto accumulate
. DO NOT useany other
in 7tr/oisopropylalcohol
be wipedcleanwith a tissuemoistened
solvent.
Top Surfacesand Case
cloth to removedust,wateror plasmastains.Thisshouldbe
Usea dry or slightlydampened
that all blood,plasmaand serumis a potential hepatitishazard.
done daify. RememLter
lnternalMechanism
in the
asdescribed
shouldnot be touclledexceptin specificinstances
The internalmechanism
to
parts
designed
are
and rnovable
sectiontitled "TroubleShooting."All internalarms,levers,
To openthis unit may invali'
and lubricated.
sealed
operatedry. The motor is permanently
your
warranty
date
22
S E C T I O NT H R E E
T H E A U T O M A T I CP I P E T T E
23
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T}-IEAUTOMATICPIPETTE
U
S
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The AutomaticPipette(BBL@CatalogNo. 60420)is a hand-heldpipettingdevicenlanufac'
dispgnses
turedspecificallyto functionwith the [email protected] devicemechanically
will allowthe AutomaticPipetteIo take
aliquotsof liquid.A quickandsimpleadjustment
designand rigidtestingaErursthe usorof
aliquots.
Careful
or
0.2
ml
up anddelivereither0.1
studies,
r$quiredfor coagulation
volumetricaccuracy
accuracy
well within the t 5o/o
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P RT N C T P L O
ES
FOPERATTON
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unit. Whenconnectedto a Fibro{retgr,ithe
The AutomaticPipetteis a modularFibroSystem
pipettewill electricallyactivatethe Fibrometerat the samemomentin eachtest.${hent[e
into the FibroTubet Cup,thereisa builf in 0.5
final bit of activatingreagentisdispensed
to 1.8seconddelaywhich is followedby the automaticdroppingof the Fibrometqrprobe
will beginautomatically.
arm.Sweeping
andtimingactivities
a
The AirtomaticPipettehasa dualPurpose:
pipettingdevice
1,.SwitchOFF - it is a conventional
- it dispenses
the Fibrometer
liquidandactiviates
2. SwitchON
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chemicalresistantplastic.All mechan'
of a light-weight,
The AutomaticPipetteis constructed
plastic.The liquidaliquotis heldin
by this protective
icalpartsof the pipetteareenclosed
(BBL
CatalogNo. 70314).Thisspecial
Tip
Disposable
from a FibroTiptt
anddispensed
prevent
liquid from touchir{gthe pipetto
tip, seatedin the gun,hasbeendesigned
disposable
uSedin
or reagpnts
item,touchesany of the samples
ting apparatus.
Only the tip, a disposable
of the AutomaticPipetteto a minandmaintenance
This keepscleaning
coagulation
studies.
performed
everytwo monfhsof use.
after
be
procedures
to
are
imum.The cleaning
t
0.2
CALIBRATION
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[[000il0
R
PLUNGE
fr
I
ON-oFF
swlTcH
;.
ALIGNMENT
IN D IC A TOR
0.1
C A L IBR AT ION
F I B R O T I PT M
lr
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Figure3'1 The Automatic flipette
25
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The AutomaticPipetteis underwarrantyfor ten yearsor until the productis no longera part
of the BBL line- whichever
is the longest.
Thiswarrantycoversdefectsin materials
andworkmanship.
The warrantydgesnot coverabuse.Replacement
unitscanbe obtainedby calling
(800)638-8663toll-free;in Marylandcall (301)666-0100
the Technical
Services
Department:
collect.
O P ER A T I N GI N S T R U C T I O N S
.
The AutomaticPipetteis suppliedreadyfor use.lt comescompletewith an electriccordand
a supplyof 200 FibroTipDisposable
Coagulation
Tips.
Independent
testing:
to testthe AutomaticPipettewithout activating
the
The followingsevenstepsaredesigned
Fibrometer:
to be cradledin the hand
1. The Pipetteis designed
on the top rest.Thisallowsfor
with the forefinger
maximumflexibilityandcontrolwhendispensing.
Manyuserspreferto holdthe Pipettein "handgun"
will producethe samerefashion.Eitherapproach
whichis
sults.A few simpletestswill determine
mostcomfortablefor the operator.
Figure
3.2
2. Insertthe plugon the endof the pipettecord into the jack locatedat the lowerleft
front of the Fibrometer.
3. Seata FibroTiP
onto the forward
end of the PiPette.
Usea firm turn'
ingmotion,as
shownhere.
%
Figure3-3
26
4. To'adjustfor the volumeof pick up anddelivery,
turn the plungerin eitherdirectionto alignthe
calibrationnotcheswith thr alignmentindicator.
Onenotchequals0.1 ml, two notchesequals0.2
ml.
Fi gure3-4
5. With the Fibrometerswitch in the ON position and the Pipetteswitch in the OFF ,
p o s i t i o n ,f u l l y d e p r e s s t h ep l u n g e r .I n s e r tt h e t i p i n t o t h e l i q u i d r e s e r v o i r . ;
;
NOTE': Be sureto fully depressthe plungerbeforethe
t i p i s p l a c e di n t o t h e l i q u i d .T h i s w i l l p r e v e n t
, aerationof the liquid and the zubsequentfor'
mation of error-producingair bubbles.
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6. lAllowthe plungerto retractfully andpick up ttrabtiquotof liquidinto the-FibroTip.
of the liqui{-reservoir
,lt is a goodpracticeto "touch off" the tip againstthe sidewall
'
tip.
i
to insurethat dropletsdo not clingto the exteriorof the
;
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the liquid into a FibroTubeCup by completelydepresingthe pluhger.The
7. Dispense
into the FibroTubeCup whib
. FibroTiplhould be inserted1t4of the *ry
festinq
belowin Fig.3'6. After ejectingthp
pipette
as
$hown
the
to
tip
ittr
closest
against
.;
'',
i
the plunger. ii
liquid,be $rre to withdrawthe pipettetip beforergleasing
27
NOTE: DO NOT let the FibroTip touch the opposite
s i d ew a l l o f t h e F i b r o T u b eC u p . A n u n e q u a l
l i q u i d l e v e lw i l l r e s u l ti f t h e l i q u i d i s a l l o w e d
t o r u n d o w n t h e s i d ew a l l . T h i s w i l l y i e l d a n
erroneousend point.
Figure3-6
TestingWith The Fibrometer?
applyto pipettingwhenthe userdoesnot wishto startthe Fibro"
The previousinstructions
to ON beforedispensing
meteraction.To activatethe Fibrometer,flip the Pipettes\,\,itch
the finat aliquotof liquid (CaCl' plasma,etc.).Electricalcontactis madeat the end of the
plungerstroke;i.e.,the instantthe final bit of liquidis dispensed.
the followingis a guidefor d Pro'
the Fibrometerhasbeentestedindependently,
Assuming
thrombinTime Testprotocolin whichthe automaticactionof the Pipettemay be tested.
TimeTest:
for the Prothrombin
the Fibrometer
1. Beginby preparing
groundedthreeprongoutlet.
A. Plugthe Fibrometerinto a standard
the
Fibrometer.
B. SwitchON
10 minutes).
C. Waitfor the indicatorlightto comeON (approximately
D. Raiseprobeto the UP Position.
E. ptacesix FibroTubecupsinto the warmingwells.Labelonecup "ControlPlasma",
fOurcups"Reagent".
andthe remaining
onecup "PatientPlasma",
2. Inserta FibroTipinto the Pipette.
3. Insertthe plugon the Pipettecord
into the lowerleft front juckof the
Fibrometer.
28
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4. Turn the pipetteplungerso that the 0.2 ml calibrationnotchesalignwith the indicator.
5. Depress
the plungerandfill'thetip with 0.2 ml of FibroTrol@Controlplasrna
(BBL
CatalogNo. 40777or BBL CatalogNo. 40778).Remember
to depress
the plungerfully
beforeinsertingtip into the reagentandthen allowthe plungerto retractfully io be
certainof pickingup the correctvolume.
;
6.De|iver0.2m|ofFibroTro|intotheFibroTuboCup|abe||ed,,Contro|P|as*',,,.
;
7. Resetthe pipetteto the 0.1 ml mode.
.
;
B. Fill FibroTipwith 0.1 ml of FibroTrol.
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9. Deliver0.1 mlof FibroTrolto the FibroTube
Cup labelled"ControlPlasma"containing
0.2 ml of the rebgent.
Thiscup will now contain0.3 of FibroTrolreagent
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10. The sameprocedures
patient
plasma,
canbe donewith
if desired.
Be sureto REPLACE
the FibroTipwith a newtip beforegoingfrom FibroTrolto patientplavna. j
11. ReplaceFibroTipwith a newtip.
,'
12. Setthe Pipetteinto the 0.2 ml mode.Fill the FibroTipanddeliver0.2 ml of Fibro.
(BBL CatalogNo. 40775or BBL CatalogNo. 40821)into oneof the
Plastin@
Reagent
cupslabelled
"Reagent".Repeatthisprocess
for eachof the threeremaining
f'Reagent"
cups.
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NOTE: lt is not necessary
to changeFibroTipTipsaslongasyou arepipettingthe samel
concentration
of a givenreagent.
In Step12,the sameFibroTipcanbe usetd
to f!ll
''
and dispensethe FibroPlastininto eachof the four cups.
F
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13. Allow threeminutesfor roomtemperature
liquidsto cometo operating
temperature.
r
Fiveminuteswill be necessary
for refrigerated
liquids.
14. Placeone FibroTubeCupwith 0.2 ml of FibroPlastin
into the center(reactiJn)*e'll.
15. fleplacethe FibroTip.
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16. Adjustthe Pipetteto the 0.1 ml mode.
I
plungerfully and insertthe
17. Witn the Pipetteswitchin the OFF position,depressthe
I
t i p i n t o t h e F i b r o T r o ll i q u i d .
1 8 . ,,Allowthe plungerto retractfully to pick up the 0.1 ml aliquot.
1 9 . Move the Pipette switch to ON .
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20. Insertthe FibroTip into the FibroT u b e C u p c o n t a i n i n g0 . 2 m l o f
FibroPlastinReagenf.The FibroTip
shoufdbe 1/4 of the way into the
cup and restingon the lip closestto
the pipette.
21. With the PipetteswitchON, dispense
the 0.1 ml of FibroTrolinto the 0.2m1
of FibroPlastin.
Ttriswill in no way invalidate
NOTE: Therewill now be a 0.5 to 1.8 secondbuilt-indelay.
the test rezults.
22. The probearm of the Fibrometer
drop.Sweeping
will automaticalty
now begin
will
actionand timing
The time for the
automaticallY.
first fibrin stranddetectionin the
reaction
FibroTrolplus FibroPtastin
and
ten
between
be
mixtureshould
plasma'
thirteen secondsfor 1OV/o
resuIts.fhen
23. Repeatthe abovetestto verify the
30
test the patient'splasma'
PRECAUTIONS
For LaboratoryUse.
?
T I PC O N T A M
INATION
The mostcommonmistake
rnadeby the userof any automatic
pipetteis the failureto
replace
+lil:T:f,iff [:ilfl:is"t ?H,';ml*p'"{l#ion,oanothe
r.Inthep,oin,Li
uin
a) Onetip for the FibroTrot
b) A new tip if patient,sprasrna
is to be tested
c) A new tip for the Fibroptastin-nrirn.
d) A newtip to dispense
the FibroTroialiquot(0..|ml)
into the Fibroplastin
cAurloN: Any tip cominginto
contactwith patientserum,prasma
or whorebroodis a
potentiafHEPAT|rfs DANGER.
Avoidpersonat
andenviron,nrn,ar
contamina.
alf FibroTipTips with the same-care
as
used
for
any
contaminated
;';Ir?j-ard
A D N I T I O N A LD E S I G NC H A R A C T E R
ISTICS
1' After depressing
the plungerto dispense,
somefiquidwilf remainin the
FibroTip.This
conditionwascompensated
for cturing
thecalibratio.of the pipette.
This
NOI-affectthe accuracy
fiquidDoES
of the Autoruai. pipette.
2' The materiafbeingpipetted
nevercontacts.*ilhthe prungeror
barrerassembry.
disassembly
No dairy
or cleaningis required.
To minimizemaintunlnr.probrems,
the
prunger
is mordecl
resistJntplastic,anohasa neoprene
rubbet "g,, ring and a stainress
J:il,i::il;:'
steel
3' once the Fibrometeractionhas
beeninitiated,the pipetteswitchcan
be movedto
oFF
i","repermits
additionar
oispensins
ofreasents
il1?:ilJrff1H"t: [',ilil:,.t:;,":r,i'
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SECTIONFOUR
T H ET H E R M A LP R E PB L O C K
33
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T H ET H E R M A LP R E PB L O C K
usE
r
An integralcomponent of the Fibro.System@
is the Thermal prep Block.
Thisprecisioninstrumenthasbeendesigned
for prewarming
largenumbersof cupsarldtubes
',
containingreagents
and patientspecimens
for Fibrometer@
testing.
'
PRINCIPLESOFOPERATION
.
The PrepBlockprovidesthirty wellsfor prewarming.
TwentyshallowwellsareFon5tructed
to hofd FibroTube@
cupsandten deepwsllsexistfor 12 x75 mm testtubes.
lne frpp Block
ensures
that liquictsareprewarmed
to the required37.2 t 0.5 C beforetesting.t1hen
irr"d
asdirected,this instrumentis an efficientandconvenient
devicefor warminglaigequanti.
tiesof all liquidsto be usedin the testsrun with the Fibrometsr.
i
i
The thermalcontrol systemof the PrepBlockhasa verynarrowdifferential.lt,phoulflnever
requireadjustment.
Solidstatecircuitrymaintains
the temperature
specifiedin liqui{sin both
the shallowand deepwellsaslongasthe ambienttemperatureis between18 artd32 p.
By utilizingthe modularconcept,d singleoperatorcansignificantly
increase
eapeof operation and work output. The followingset-upis zuggested:
i
i
1 . Beginwith two FibrometerTimersand oneThermalPrepBlock.
2. Connecta Fibrometsrto eachsideof the PrepBlock.
, 3 . The removableplastictrays of the PrepBlock can be pre-filledwith reAgentsiand
refrigerated.
f
I
4. The instrumentscan be brought to operatingtemperatureand the trayf of reagentr
can be pfacedover the shaffow wefls.
i
,
5. The reagentscan be brought to the testingtemperaturein five minuteq Duripg this
time, additionaltrays can be readiedbnd refrigerated.
r
Continuous
testing
and
reagent
warm-upcan now beginwith no risk of deterioration
, 6 .
due to prolonged heatingof reagents.
I
.
.
r l
35
S H A LLOWW ELLS
( For F broTubs oD isposableCupsl
FIBROMETER@
D E E PW E L L S
b
ffi
re
IN DIC A TORLA MP
ON .O F FS W ITC H E S
T H E R M A L . P R EB
PL O C K
PLASTIC
SELF.STACKING
T R A Y SF O R F I B R O T U B E @
CUPS
D IS P OS A B LE
A U T OMA T ICP!P ET T E
Fi gure4' 1 The C omP l eteS Y stem
36
THERMALPREPBLOCK
Performance
characteristics
andspecifications
I
TemperatureRange
Volumes
37.2r 0.5c
Up to 0.5 ml in FibroTubso cups in twenty shailow
wells
r
i
Up to 3.0 ml in 12 x75mm testtubesin ten deep
wells
.
D i m e n s i o n sa n d W e i g h t s
Height - 102mm (4")
Width - 159mm (6.25,,)
Length - ,|84mm (7.25',1
Net Weight- 2.9 kg (6.4btb)
Shippins
Weight- 4 kg (9.0tb)
B l o c kW a r m - U T
pime
Approximatelyfifteenm inutes
L i q u i dW a r m - U T
pime
Refrigerated
liquids:five minutes
Roomtemperature
liquids:threeminutes
ElectricalSpecifications
117 volts,50 or 60 Hz,0.6 ampere
C O M P O N E NPTA R TP RI N C I P L EO
SF O P E R A T I O N
HeatihgSyStem
A heatingcircuit, regulatedby a precisemercury thermostat,holds the warming bfock
at
r
37.2t 0.5C.
:
i
Heating Block
An epoxycoated,durablealuminumblockprovides
precision
heattransmisiont9 liquids
anywherein the block.
IndicatorLamp
Whenthe unit is pluggedin andthe srruitch
is in the ON position,the neonindicatbrlightwill
comeon immediately.
The indicatorlightwill remainon to indicatethe heatingcircuit;is
functioning.Whenthe warmingblock reaches
a temperature
of 37.2t 0.5 C, the ihermbstat
will switchout and the light will go off. Whenthe thermostatcallsfor heat,the indicatbr
lightwill comebackon.
:
i
NOTE: The actionof the ThermalPrepBlock indicatorlamp is not ths same.. in, fibromotsr indicatorlamp.In the Fibrometer,the indicatorlampis designed
io glow
when lirCuids
reachthe requiredtemperature,
Remember:
1. Fibrometer- machineON, light ON, liquidsreadyfor use
I
2. ThermalPrepBlock- machineoN, light
9FF, liquidsreadyfor use
37
Power Supply
The Thermal Prep Block Tay be operatedindependentlyby attachingthe unit by a three-wire
line cortJ(not supplied)to any approved11O-volt,three-wiregroundedreceptacle.
Most usersconnectthe Thermal PrepBlock directly to the Fibrometer.Both of thesemachines
havebeendesignedto operatein a modular arrangement.A singlepower sourcewill operate
both machines.The secondmachinein line will receivepower evenwhen the first machineis
switchedoff.
Safety
The Thermal Brep Block is certifiedfor safety by the Los AngelesCounty Testing Laboratorieg
and is listedby the CanadianStandardsAssociation(CSA).
DO NOT separateor connect the Thermal Prep Block to the Fibrometer while the line cord is
connectedto the power receptacle.A Shock Hazardexists when doing so.
INSTALLATION
EquipmentModeland PowerRating
g o . 6 0 4 1 9 T h e r m aPl r e pB l o c k 1 1 7v o l t s ,5 0 o r 6 0 H 2 , 0 . 6a m p s .
B B L C a t a l oN
LaboratoryRequi rements
on a standard117volt A.C. powersourcewhichmustmeet
The ThermalPrepBlockoperates
of 99.5to 128.7volts.The outtet
standard
Association
the NationalElectricalManufactures
(wall or throughthe FlbrU'
grounded
receptacle
must be a standardlaboratorythree-wire
The workingenvlronm6Rt
the machine.
or outletsmay damage
meter).Otherpowersources
between18 and32 e
temperature
an
ambient
with
possible,
as
dust-free
and
shouldbe asclean
(64.4to 89.6 F).
Procedures
:
{
a
I
l|
t
I
'r|o*
you'to test your new ThermalPrepBlock. lt is
ib design.J1o
The followingprocedur.C
havebeentestedindependently.lf any
Pipelte
assumedthat the FibromatqruriOAutqmatic
plqasecallthe BBL Hot Line,toll'
you testyo.prne\,r{.Trjr?!T,1l.P.tj'8,,P]:9!,
probremgoccur.as
:
in Marylandcall (301)666'0100collgct.
irr"t (B0O)GpA,gOO3;
t- gqsin
byptacins
lheJqg':mal
t1eP."Qjo.-q,k
P:l yilh lh9Tt9,?,*^TjIll,':-lI?:I
the UP
38
a
-
3 . T o u t i l i z et h e m o d u l a rp l u g - i nd e s i g n ,
set the Prep B lock to the right of the
Fibrometer. Carefully connect the
three-prongedoutlet on the right side
of the Fibrometer to the receptacle
on the feft side of the prep B lock.
WARNING: DO NOT twisttheseoutlets.
4.. Plug in the lirie cord from the Fibrometer and put the power switch of
the Prep B lock into the ON position.
Waitapproximately
fifteenminutes
for the machineto reachoperating
temperature.
The indicatorlightwill
be ON while the machineis warming.
At operatingtemperature,
the light
w i l l b eO F F .
6. The ProthrornbinTirneTestcannow berrunto testthe performance
of thd Thermal
Prep B lock.
A. Check that both instrumen'tsare at operatingtemperature.
B. Placefour.FibroTubecupsinto- :''
one of the removable
plastictrays.:^,
Labelone cup FibroTrol@and. . ,
the remaining
threecups
"Fleagtnt".Placethe tray into the
' -:
w e l lo f t h eP r e pB t o c k . : - : ' '
C . U t i l i z i n gs t a n d a r do p e r a t i n gp r o cedureswith the Automatic
Pipette,fill one cup with 0.3 ml
FibroTrol and the remaining
cupswith 0.2 ml FibroPlastin@
Fleagent.
D. Wait threeminutesfor roomtemperatureliquidsto arriveat the testingtemperaturs.
liquidsfiveminutes.
Allow iefrigerated
E. Placeone cup with 0.2 ml of
FibroPlastininto the center
(reaction)
well of the Fibrometer.
with the PipetteswitchoN, de'
liver0.1 ml of FibroTrolinto
the cup.
F. After the built-indelay,the probearm of the Fibrometerwill fall andthe sweeping
andtimingactionswili begin.The time for the first fibrin stranddetectionshouldbe
betweenetevenandthirteenseconds'
to verifythe results.
G. Repeatthe aboveprocedures
sdt'
you arereadyto beginroutineuseof the ThermalPrepBlock.ThereareNo temperature
instrument'
with thisor any otherFibrosystom
necessary
Jaliurations
tingsor day-to-day
O P E R A T I NH
GI N T S
plastictray. Two traysaredesigned
1. Ten FibroTubecupsmay be placedin one removable
this position,the cupsreston the
to fit snuglyacrossthe shallowwellsof the PrepBlock. ln
the trays.The traysdo not affect
sphericalbottornsof the wells,just asthey would without
the temperatureof thq cupand its contents'
i
at37.2 C doesnot disturbthe
liquids
with
wells
to
2. ptacingcold liquidsin wellsadiacent
thermalequilibriumof the heatedliquids'
A box of ten trays(BBL
for the next d..y canbe preparedand storedovernight.
3. Reagen_ts
ih, rcquirednumberof cupscanbe placedin the
catalogNo, 70313)mav be purchased.
traysstoredovernightat 4 c' The
trays,0.2 ml of reagentaddedto eachcup,and the readied
traysare madeto be stackedto uselessstoragesp3ee.
M A I N T EN A N CE
PRECAUTIONS
F o r L a b o r a t o r yU s e .
The Thermal Prep Block is easyto keepclean.Wipe the metal surfacesand wells with a damp
cloth . Be sure to take all necessry precaations to avoid hepatitis dangers when working with
human serum,plavna or whole blood. The wells should be wiped periodically.
There are no temperaturecontrolsor adjustmentsof any kind in or on the Prep Block. The
h e a t i n gc i r c u i t i s e x t r e m e l ys t a b l e .l f t h e r e i s a b a d c o m p o n e n ti n t h i s c i r c u i t ,t h e b l o c k w i l l
b e c o m et o o h o t t o t r o l d o r w i l l b e w e l l b e l o w3 7 C .
41
S E C T I O NF I V E
STANDAR
D OPERATINP
GR O C E D U R E :
PROTHROMBT
I NI M ET E S T
60p
t
43
P R O T H R O M BTI N
I M ET E S T
i
i
This sectioncontainsa step-by-step
procedureto test the FibroSystem@eqripment intheprothrombin Time Test. lt is recdrnmended
that beginningpersonneland new usersof the FibroSystemwork their way throug$r
SectionsOnethroughFour to independently
test equilpment
and learnprocedures.
I
'
i
l
1' Connectone FibrometsroandoneThermalPrepBlockin modularsequence.
placeths
Fibrometorto the left of the PrepBlock.Carefullyconnectthe three-pronbed
rorpt.rl.
'
on the rightsideof the Fibrometer
to the pl6gon the left sideof the i*pbtocf.
2. Connectthe line cord to the three-pronged
plugon the left sideof the Fiblomotrr.Be
certainto matchthealignment
dotsfoundon both the linecordandthe Fibromstor
plug.
.
3. Plugthe linecord into a standard
laboratory
grouncled
three-wire
outlet. :
4. SwitchON the Fibrometerand the PrepBlock.Both machines
shouldbe qt oper:ating
temperature
I
in approximately
i
fifteenminutes.
5. Lift the Fibrometerprobearm to the Up position.
6. Plugthe Automatic Pipettecord into the lower left front receptacle
of the Fibrgmuter.
Leavethe Pipettesrritchin the OFF position.
i
i
7. PreparetheFibroTrol@
testsolution.You will need0.1 ml foreachtest.Asumethat
you will run TWO tests.Usingthe AutomaticPipette,take up 0.2 ml of FbroThl and
dispense
into a FibroTube@
Cup labeled"Control',.}
i
i
It Placethis cup into a plasticrackandsetoveroneof the shallowwellsin tlie Pref Block.
,DothisafterthePrepB|ockhasreachedoperatingtemperature
9. You will need0.2 ml of FibroPlastin@
per test.Assumeyou arerunningTWO tgsts,
Usingthe AutomaticPipette,takeup and dispense
0.2 ml of FibroPlastin
lnto ohe cup
a secondtime for the second"Riagenl".rp.
. labled"Reagent".Repeatthis procedure
NOTE: A new FibroTiprMshouldbe usedfor the FibroPlastin.
DO NOT uset+ sametip for
FibroTrol and FibroPlastin.
!
,
1 0 . Placeboth "Reagent"cupsinto the rackovera shallowwell in the Prepgtck.
1 1 .It will take three minutesfor room temperatureliquidsto reachthe test tdmperbture
( 3 7 . 2 t 0 . 5 C ) . A l l o w r e f r i g e r a t e ldi q u i d sf i v e m i n u t e s .
t
.
1 2 . Once the liquidsare at operatingtemperature,placeone "Reagent" cup irito the center
(reaction)well of the Fibrometer.
rl
I
;
l
Be certainto replacethe FibroTip in the Automatic Pipette.Switch the pipetteiinto
t h e 0 . 1 m l d e l i v e r ym o d e . D o t h i s b y t u r h i n g t h e p l u n g e ru n t i l t h e s i n g l en o t c h b t i g n s
with the indicator mark.
1 4 . Take up 0.1 ml of prewarmedFibroTrol with the Automatic Pipette.
;
1 5 . Switch the Automatic Pipetteto the ON position.
I
'
,
t,
l
tJ
t ltissuggestedthatyouaddanaddition
0a
. 1l m l o f F i b r o T r otl o t h e c o n t r o lc u p ' t o g i v e r tiib t a lv o i u m e o f
;
l
*
0.3 ml. This will avoid pipetting the cup dry on the secondrun. ;
i
,i
ml of FibroTrolintothe "Reagent"cup nowsittinginthe Fibromoter
16. Dispense0.l
well.
reaction
delay.Thiswill allowyou to removethe
NOTE: Therewill be a Suilt-in0.5 to 1.8second
FibroTipfrom the FibroTubecup.This delaydoesnot changethe test results.
and
17. The probearm of the Fibrometerwill drop automaticallyand beginthe srrueeping
timingactions.
The
18. First fibrin stranddetectionshouldoccurbetweenelevenand thirteenseconds.
Fibrometerwill stop timingand electrodeactivityautomatically.
the red "reset" button for at least
1g. Recordthe time and resetthe timer by depressing
probe
arm'
the
then,lift andclean
one full second;
20. Replacethe FibroTipand discardthe FibroTubeCup from the reactionwell of the
a
Fibrometer.
Steps11'2O,to verify the results.
21. Repeatthe aboveprocedures,
patientserum,
WARNING: Be certainto carefullydisposeof all tips and cupsthat contact
gecimeni
plasmaor wholeblood.Thereis a HEPATITIsDANGERwhenworkingwith these
or concentrationbeing
NOTE: Becertainto changethe FibroTipfor eachchangeof liquid
pipetted.For example,Do NOT usethe sametip for both FibroTroland FibroPlar;tin.
46
S E C T I OS
NI X
p R E v n i r r r v EM A T N T E N A NpcREo c E D U RFEo R6 0 4 2 0& 6 c y , z z
A U T O M A T IP
CI P E T T E S
hunts
fuaffipfuH
47
i
I
?
l
t
H E L P T YH. | N T S
'
i
l
i
Theprocedure
outlinedin thissectionis designed
j
to preventtip fallout and leakagewhen
usingthe BBL@Catalog
- No.60420AutomaticEtectricPipette
or
BBL
Catalog
--,
'-r ' frfo
! v ' I 6O+ZZ
NonelectricPipette.
The twistingmotionof inserting
the disposable
plasticpipettetip into the barrelof
{thepi-i
pettemay leavebitsof plasticadhering
to the sidewallsofthe holein whichthe tipis in- ,
serted.Thisprevents
the tip from seating
properlyandmay resultin tip fallout.
f
;
Cleaning
the "O" ringandcannulais requiredto preuentdeformationof the soft "O,, ring
asit ridesoverdirt particles.
Deformed,
worn ordamaged"O" ringsmay permitair,toenler
and resultin leakage
of materialfrom the tip.
t
BBL CatalogNo. 70312FibroTube@
Disposable
PlasticCoagulation
Cupsarespeciti..ffvl
recomnlended
for usewith the Fibrometer@and AutomaticPipettesbecause
they aredesigned
to providepreciselythe liquid levelheightreguiredby the Fibromoterfor accurateoperation.
The BBL CatalogNo. 70314FibroTiptt is precisely
engineered
andrigidlyqualityiontrolled
for a leakprooffit in the 60420and the 60422pipettes.
i
i
I
!
I
.
i
49
M A IN T E N A N C E
1 . H o l d t h e p i p e t t ea so n e w o u l d w h e n
With the other hand, graspthe
dispensing.
metal barreland pull it straightoff the
pipette.
2. Takethe metalbarrelto anotherbench
awayfrom the plasticparts.Placea small
quantityof acetoneon a swabandvigorouslyscrapearoundin the holewherethe
tip i; placed.Thiswill cleanout the plastic
from normal
bitswnichhaveaccumulated
usage.
3. Usea freshswabmoistenedwith alcohol
and swabout the centerhole in the metal
barrel.Usea smallswaband keepcleaning
until the swabcomesawayclean'Rinse
the barrelandcannulawith distilledwater
Dry
to washout any particlesthat remain'
thoroughlY.
50
4 . T u r n t h e p l u n g e ro f t h e p i p e t t et o t h e
0 . 2 m l s e t t i n ga n d d e p r e s st h e p l u n g e rf u l l y .
T h i s w i l l e x p o s et h e " O " r i n g .V t | i t ha l i n t '
lesstiszuemoistenedwith alcohol,clean
the areaaround the "O" ring and the "O"
r i n g i t s e l f .W h e nc l e a n ,v i z u a l l ye x a m i n et h e
"O" ring for slits,flat spotsand other signs
of wear. lf there is damage,replacethe "O"
ring or requesta replacementfrom BBL.
5. Placea smallamountof siliconegrease
or petroleumjelly aroundthe "O" ring.
With the pipetteplungerstill depressed,
sfide the pistonand "O" ring into the tube
of the metalbarrel.Align the barrelso that
the notchon the perimetermatchesthe
projectionon the pipette.Snapthe barrel
in place.
6 . W o r k t h e p l u n g e ru p a n d d o w n t o l u bricatethe tube. Take a tip, insert it in the
pipette and pick up 0.2 ml of water. Hold
the pipette steadyfor one minute without
touching the plunger,watchingthe end of
the tip to seethat no drop,forrns''Repeat
on the 0.1 ml setting.
. '-
51
SECTIONSEVEN
CUPS
U S Eo F F I ' * o ' u ' E @ D I S P O S A B LPEL A S T I CC O A G U L A T I O N
R E c t s t o Nc o A G U L A T t o NT T M E R
w t T H T H E F T B R o M E T E Rp@
53
H E L P F UH
L INTS
,
i
The FibroTubeCup is an integrplpart of the Fibrometerbecause
it preciselyandcensistently
presents
plasticcup'ispr6the sampleto the Fibrometsrprobe.The FibroTubedisposable
tectedunderU.S.Patent#3,219.421.The Patentwasgranted,basedon the dimeniionsof
the FibroTubecup,which enables
the cup to performits precisefunctionin the Fibromster.
The internaldiameter(1.D.)of the FibroTubecup,fixestheliquid levelat a preciseheight
whena total'volumeof 0.3 ml is placedwithin the cup.The ryhericalradiusof the bottom of
the FibroTubecup exactlymatchesthe sphericalra{liusof the tube well of the Fibiomstor.
This fact, in concertwith the uniformwall thicknessof the FibroTubocup,providasexcellent
heattransferfrom the heatingblockto the liquidwithin the cup.
t
i
t
:
L
1
Figure7-1, showsa cutaway
view of the heat block, probe
assembly,and associated
clot detectionapparatus.In
this f igure,a clot has been
detected and the Fibrometer has stopped.
fr
i
MOVTN9
ELECTRODE
STATIqNARY
qLECThODE
in thd cup.
The irobe or electrodefoot
is contactingthe camcarryingthe signalthat a clot is
present,to the cam,cam
finger,an-ddetectioncircuit.
l
BLACK CAP
The moving electrodehas
m e c h a n i c a l l cy a u g h ta f i b r i n
strand during its srueepthrough
the m'aterialin the cup and
then lifted to a point 1.27mm
the levelof the liquid
abovei
t
At th.istimg andonly at this
time the movingelectrodeis
Glrically active.
l
PROBE
FOOT
CONTACT
FINGER
DETECTION
CIRCUIT
Figure7-l
;
55
f n F i g u r e T - 2 ,t l r e m o v i n ge l e c t r o d ei s m e c h a n i c a l l ys e a r c h i n g
f o r a f i b r i n s t r a n d .T h e e l l i p t i c a l
path taken by the movablcelectrodeis directed
and controlled by the groove in the blabk cap
where the upper portion of the electrodeis
captivated.
The probe foot is restingon the insulatedport i o n o f t h e c a m . T h e m o v i n ge l e c t r o d ei s n o w
'
electricallyinactiveand cannot conduct. As
the cam continuesto rotate, the moving electrode is lifted by the contact portion of the
cam and the configurationreturnsto that of
F i g u r e7 -1 .
NOTE: The moving electrodeis alternatelyin
the up or down position every half
second,stoppingFibrometeronly
where there is a clot to completethe
circuit.
FigureT-2
lmportant: lf 0.3 ml total volume is placedwithin a competitivecup whose internaldiameter:i
than
smarrer
issrishtly
ll'eil.ter?.]_d':T:::"jJl"'^:.?::j::1".1$:,?i1,1li^liiloff1l'*,
tofiEhfilA;il-and bridgethe 1.27mm gap,therebyclosingthe circuit causingthe Fibro' ,'l
drop into the cup (Figure7'3). ConvOrfrly;
meterto stopalmostassoonasthe probeelectrodes
if a 0.3 ml total volumeis placedwithin a competitivecup whoseinternaldiameterisgfufily :
belowthe levd
thenthe liquidlevelwill be considerably
largerthan the BBLo FibroTubeocup,
for the
sucha low liquid level,it is possible
ffiTneu in the [email protected]
Fibro'
the
the
circuit;
breaking
the
cup
in
liquid
the
above
to lift the clot
movingelectrode
BBL0
of
by
use
rectified
problems
best
are
meterwilt not stop(Figure7-3).Bothof these
FibroTuue@
,
The problem of short times or a "no detect" condition can also be causedby maladjusted
the
cleanirq
by
lengthened
being
electrode
moving
the
by
electrodes.Short times can be caused
l,
procedureovera periodof time.The normalcleaningprocedureafter eachtest is to raiseth€
down with the lintlesstiszue.Depending
probeto the restpositionandwipe the electrodes
of "squeeze"exertedupon the electrod0,
uponthe lengthof the time in serviceandthendegree
uy 0.64 or 1.30mm. Thisconditioncanbe
the movingelectrood
to lengthen
it is possible
towardthe ceilingandthq
correctedby holdingthe probein your left handwith the blackcap
you will noticea
body,
At the very bdttom of the cylindrical
facingyois rigi"rt.
electrodes
probefoot' Graq
the
is
this
singlel,Euvywire whichcomesout straight6.35 mm thencurves;
force.while you arepulling
the probefoot with your righthand'andpull downwith a steady
seeit moveslightlyforward
down,watchthe hook end of the movingelectro$e.You should
M O V I N GE L E C T R O D E
I N R A I S E DP O S I T I O N
C O R R E C TB B L @
@
FIBROTUBE
C U PF L U I D L E V E L
I N S I D ED I A M E T E R
TOO S MA LL
I N S I D ED I A M E T E R
TOO LARGE
Figure7-3
a n d u p . T h i s s h o r t e n st h e e l e c t r o d eb y 1 . 3 0o r 1 . 9 3 m m . T e s tt h e a d j u s t m e nbt y p l a c i n g0 . 3
ml of reagentgdy in a FibroTubecup in the centerwell of the Fibrometer.Placethe probe
i n t h e F i b r o m e t e ra n d p r e s s t h et i m e r b a r s o t h a t t h e p r o b e w i l l d r o p i n t o t h e r e a g e n t l.t
should continue to run. In the eventthat the unit againstopsprematurely,the procedurecan
be repeatedone more time. lf the problemcannot be resolved,call the BBL TechnicalServices
t o l l - f r e eH o t L i n e a t ( 8 0 0 ) 6 3 8 - 8 6 6 3 i;n M a r y l a n dc a l l ( 3 0 1 ) 6 6 6 - 0 1 0 0c, o l l e c t .
A "no detect" condition can be causedby the accidentalbendingof the moving electrode
a w a y f r o m t h e stationaryelectrode.This shortensthe electrodecausingthe "no detect" condition.
d i t h a p a i r o f f o r c e p s .l f t h e t h i n w i r e
l f t h e t h i n w i r e s e c t i o ni s b e n t i t m a y b e s t r a i g h t e n ew
s e c t i o ni s s t r a i g h tb u t t h e t u b i n g i s b e n t , i t c a n u s u a l l yb e c o r r e c t e db y p l a c i n gy o u r t h u m b s
t h e t u b i n ga g a i n s t h e c a p .
a g a i n s t h e t u b i n g a t t h e b l a c kc a p a n d p r e s s i n g
Another factor that can causea "no detect" condition is that the automaticpipette is dirty
a n d n o t d e l i v e r i n gf u l l v o l u m e . C l e a n i n gt h e p i p e t t ea s d e s c r i b e di q S e c t i o nS i x , w i l l r e s t o r e
t h e v o l u m e t r i ca c c u r a c y .
57
SECTIONEIGHT
. S H O O T I NA
TROUBLE
GN D R E P A I R
59
T R O U B L E- S H O O T I N GA N D R E P A I R
T h i s S e c t i o ni n c l u d e st h e f o l l o w i n g :
1.
2.
3.
4.
5.
Flowchartsfor trouble-silootingthe Fibrometer@
Additional problemswith the Fibrometer
Trouble-shootingchartsfor the Thermal prep Block
Trouble-shootingchartsfor the Automatic pipette
Repairproceduresfor the FibroSystemo
t
I
I
ln the past,we havefoundthe followingtypesof irldividuals
doingtrouble-shooting
and
repair:
i
;
1. The technicianwithout any experiencebeyond operatinglaboratoryequipment. lf
you fall into thiscategory,DO NOT attemptinternatrepairs.
Whenworkingwithrthe
Flowcharts,
STOPat the largeblackdot andseekexperienced
helpfrom thb repair
, technicianwhere you work or through the BBL Hot Line.
2. The technicianwith trainingto makeminor repairs.lf you fall intothiscategory,you
mustbe the judgeof the levelof trainingyou haveacquired.
Obviously,
complex,circuit
work, thermostatelectrical
,
evaluation
etc., aremoreworkof the specialist.
i
Whenever
you arenot sure,consultwith the repairtechnician
whereyou work, or call
tne BBL Hot Line
i
3. The trainedrepair technician.Most repairson FibroSystemequipmentarewithin the
rangeof activitiesperformedby trainedrepairpersonnel.
Shouldtherebe any questions,
t callthe BBL Hot Line.Our technicians
andengineers
will be happyto assisiyou.
Please
notethat a wiringdiagramandmechanical
diagramhavebeenincludedin this manual.
A completepartslist hasalsobeenprovided
j
i
N O t T : B E F o R ES T A R T I N G
A N Y R E P A I R Sc ,H E c K Y o U R W A R R A N T Y .
,
;
o B S E R V EE L E C T R I C ASLA F E T Y- U N P L U GT H E P O W E R
q
E
F
O
RE
CORD
i
STARTING
,
ANY REPAIRS.
A L S O ,W I P ET H E M A C H I N E
T O R E M O V EA N Y P L A S M AR E S I D U EA; . H E P A T I T I S
.
I
D A N G E RE X I S T S .
61
ART NU
FLOWCH
e a i l st o W a r m
N O P O W E R : M a c h i nF
andMotorWill Not OPerate
PROBLEM:
Fi
I
cord
,:J
,*n*:
cordNot
In
Plussed
t
I
I
I
Plug In Cord
to grounded
3-wire recaPtrcle
t
switchisoFF
or NotFullYin
ONPosition
I
I
I
i,*JTn
l
FIR MLY movet hs
Power Switch to the
ON position
r
I
lF;" lsGood
/[*r[i$'i'V
I
I
R, thir
CanreplaceHOWEVE
is an indicationone or moro
Machinecomponenr have
failed.
c
a
Fuseis Blown
C a l l BB L
Call BBL Hot Line
t
62
-4
P R O B LM
E:
FLOWqHARTNU
NUMBER
2
Mactrineis Not Activated
by Automaticpipette
i
i Fibrometero
Fibr
t
,
t
l
i
)
I
_4T"
SwitchesON
t
'
Ny
t
:
:
-
Switch(erloFF
/
/
Both the Fibromete.rnJ Automatlc
PipetteSwitchesMUSTF€in tre
ONposition
,;
Pl u gi s
Secured
Properly
Plugis Lbose
:
Plugthe Pipettecord
securelyinto the frgnt
jack of the Flbrometsr
Ir
w i th T i me r
MachineActivates
MachineDjcs ruoq
Activate :
:
,
Pipetteis fautly
CallB B L fo r
Fn Ee REPLAcEMENT
i
?
'
Call BBL Hpt Lino
.a
I
63
'
FLOWCHARTN
PROBLEM:
ProbeFailsto Drop
t
CheckPower
\-
\
r//
Power
/
NoPower
I
F L O W C H A RNTU M B E R
\s.;,ry
pn1c.t
I
!',s;
I
clean
Probe
/
I
#il'' /
I
Removeall accumulateddirt wi$l
lintlesstirsue moistenedwith 70i6
IS OP R OP Y L
A LC OH OL
Cleangroovc at rear of probe and
plastic sleevein probe hole
<D
Probe support
lanerNot Bent
/
/
/
lJ,"F,Trfl^
/
t
S E ER E P A I R
P R O C E D U R1EB
S E ER E P A I R
1A
PROCEDURE
I
64
,
I
FLOWCHART
, M B E R4 A
NUM
PROBLEM:
Overheating
MoreThan37.7C
i
a
r
1
,
r
t*n,ne tsNr
/
I
lnT;:F;eil-\f"
f
brometera
i
F
i
j
tr bn
Machine
I
| ffiffffI*^ /
Move one of thes€
instrument$to a
different bencfr or
,
insulatewith
lsomode Pad
! ; ,
S E ER E P A I R
P R O C E D U R4E
-ft;$
-rrr-gutuL-.2
I
I
l
r
t
.
S E ER E P A I R
PROCEDURE
I
I
I
I
Thermostat
ls N o t F a u l ty
/
Thermoststls ,
Faulty:
i
I
I
Ir
ReplaceT[ermostat
70585 and70609 or
return to QBL for
repair
r
!
CallB B L H o t L i n e
t '
f
l
I
,
'
i
{
NOTE:
l
f the heatihgsystemis faulty the warmingblock will usuallybe too hot to Frth qr will
off
be below37C. Thereis little chanceof a PrepBlock beingonly one or two degrees
the desiredrang€.
r
{
r
65
1
N
FLOWCHART
PROBLEM:
FibrometerNot HeatingAt All
Mrchinslr Not
RmoivingPrnrsr
Machine
Receiving
Power
SEEFLOWCHART
N U M B E R1
S E ER E P A I R
P R O C E D U R3E
Thermostatls Faultl
Thermostat
lsNot FaultY
ReplaceThermostat70585
and 70609 or return to
for repai r
CallBBL Hot Line
66
FLOWCHART
NUMBER4C
PROBLEM:
F.brromatcro
FibrometerHeatingto LessThan36.8 C
i
l
a
a
/ +*T::ji*
I
;/ n*oiunt Terrr
I *::Tl;Eo**""f
/
Too Lowl
i
l
l
Regulate Amgient
i
petwegn
Temperature
l8Cand32$
- z \
l
: RE P A I R
)CEDURE
I
!
/
Thermostat' s /
Defective'
u.
l^,i'JitsT?i".
/
/
I
Replaceentire thermostat
70585 and 70609 or send
l
to BBLfor reOafr
CallB B L H o t L i n e
r
{:
i
,
,f
t
t
t
.
a
t
t
i
;
f
,t
(
I,
j
.l
J
I
:
67
:
t
I
I
l
t
T
i
"
;
,
'
FLOWCHAR N
PROBLEM:
FibrometerDoesNot Stop with Fibrin
Formation;DoesNot Detect End Point
I il"fr'"T"
I
Fi
/
Efectrode
t
Misaligned
I
I
I
Hand-align tho Eloctrod€s
so that they ar€ Parallelto
ProbeBody and reasonablY
straight
Check
V o l u m ei n
lnsufficient
V ol ume
Sufficient
Volume
SEEMANUALSECTION
Hints)
SEVEN(HelPful
Check
Cam
(76010)
I
C a l lBB L H o t L i n e
S E ER E P A I R
P R O C E D U R6E
S E ER E P A I R
P H O C E D U R6E
FLOWCHAFTNI U M B E R
6
PROBLEM:
S f r o r tT i m i n g ,o r M a c h i n eS t o p s l m r n e d i a t e l y
or 0.5 Sec.after ProbeDrop
prometer@
Fibr
o
y,x'ff';[
i:i;"*
I
1
Electrodes
a,".n
/
,
I
i
r
Wipe ElectrodesClopn
with lint-freelab grqde
tissue
,
i
;
I
<-D
f o"u^rlated
Reaction
Well Clean
i^'^/
| f,:HttTT,Xl
Cleanwith lintlfree tissue
moistenedwith lsopropyl
Afcohol
t
i
I
I
t
t
,1
r
,
It
Electrode
Length
S E ER E P A I R
P R O C E D U R7E
I
Electrode LPngth :
Needs Adjuetment;
Electrode Length
ls Correct
, l
t
t
t
I
;
!
S E ER E P A I R
PROCEDUR
7E
{
:
C a l lB B L H o t L i n e
69
TROUBLE- SHOOTING:
F I B R O M E T E R, @
ADDITIONAP
L ROBLEMS
I
Listed below are problemsthat may occur with the Fibrometer,but havenot been
coveredin the f lowcharts.
PROBLEM
SOLUTION
1 . P r o b ew i l l n o t r e m a i ni n t h e U P
Position.
1. SeeRepairProcedure
#1
ProbableCause: Bent probe $Ipport
'
lever.
in test proceduret
2a.Any inconsistency
or reagents
will causeerratictiming.
SeeSectionll.
2b.lf the problemis low linevoltage, :
Requirecheckthe Installation
ments,Sectionll. In addition,have
periodically
checkline
an electrician
voltage.
2. E r r a t i cE n d P o i n t s
ProbableCause: This condition is
r a r ea n d i s u s u a l l ya t e c h n i q u e r, e agentor line voltageproblem.
3 . S t i c k yT i m e r o r D i g i t R e p e t i t i o n
3. SeeRepairProcedures
#10 and#11
4 . M a c h i n ew o r k s o n P r o t h r o m b i nT i m e s
but doesnot pick up clot on PTT's.
4. SeeFlowchart#5
TROUBLE. SHOOTI
T H E R M A LP R E PB L
SOLUTION
PROBLEM
1 . M a c h i n ew i l l n o t h e a t
ProbableCauses:
1a.Firmly movethe powerswitchON.
l b.Thisproblemmay occurwhenthe
PrepBlock is connectedto the
A . M a c h i n en o t s w i t c h e dO N .
B. Faulty power connection.
#12.'
motor.SeeRepairProcedure
2. The heatingelementand systemof
b
the PrepBlockis almostidentical
lowcharts
the Fibrometsr.SeeF
#4A to 4C. Also seeRePairProced'
ure#3.
TemPerature
2. Erroneous
70
:
. SHOOTING
TROUBLE
AUTOMATIC
PIPETTE
t
t
Many of the problemswith the Automatic Pipettecan be solvedby reading SectionVl:
H e l p f uI H i n t s .
PROBLEM
SOLUTION
1. Fibrometer is not activated by the
Automatic Pipette.
1. SeeFlowchart
#2.
2 . P l u n g e rb i n d so r w i l l n o t d e p r e s sf u l l y .
'
ProbableCauses:
A. D irty cannula
B. Worn"O" Ring
2a.SeeSectionVl
2b.SeeSectionVl
3. Leakageof liquid from tip.
ProbableCauses:
A. Wqrn or defective"O" Ring.
B. lmproper seatedor defectivetip.
3a.SeeSectionVl
3b.Firmly securethe tip into Pipette.
Checkthe FibroTiprMfor obviou,s
flaws.
,
4. Inaccurate
delivery
ProbableCauses:
4a.Seepipettingdirectionsin gectionlll.
I
4b.SeeSectionVl
A. lr4propertechique
B. Dgfective"O"Bing
;
I
I
I
i
71
REPAIRPROCgDURES
B E G I N N I NA
GN Y R E P A I R S
N O T E : R E A DW A R R A A J TBYE F O R E
O N L YT R A I N E DP E R S O N N ESLH O U L DA T T E M P TI N T E R N A LR E P A I R S
I C A LS A F E T YG U I D E L I N E S
W A R N I N GO
: BSERVELECTR
Fibrompter@and Tlrermal Prep Block
1 . P r o b ef a i l st o d r o p o r i t w i l l n o t r e m a i ni n ' t h e U P p o s i t i o n .
A . C h e c kt o s e ei f t h e m a c h i n eh a sa f a u l t y t i m e d e l a yc i r c u i t o r s o l e n o i d .
,
I
1 ) R p m o v et h e P r o b e .
i
2 l A c t i v a t et h e m a c h i n e .
probe
3) Watch for the probe support lever(brassleveron the right hand side of the
hole) to snapinsidethe sleeve,out of sight'
4) lf the leverdoesnot retract,the machinehas a bad time delay circuit o,rsolenoid.
Factory repairwill be done by BBL. lf desired,a faulty solenoidmay be replaced
with a new solenoidassembly(BBL CatalogNumber 76007). The timri delay
capacitor,if found defective,may be replaced(BBL CatalogNumber 7061q)'
B. lf the leverdoes retract, and the probe sleevehas been checkedfor dirt acclmulation,
approximately,l.6 mm
I the levermust be out of alignment.The leverarm should extend
'
into the probe hole oeyond the sleeve.lf it extendsmore than 1.6 mm:
1) Use a 6.4 mm bladescrewdriverto pressthe leverback into the cavity. Pressuntil
a stoP is felt.
in the lever.
zl Careiully apply 2.3 to 4.s Kg. of pressureto force a slight bend
the
desiredpoint is
if
back
bent
be
The leveris malleablebronzeand may easily
;
overshot.
is
achieved'
1.6
mm
desired
the
until
procedure
Repeat
3) Check the extension.
the leverand
4l' lf the leverextendslessthan 1.6 mm, put the screwdriverbehind
apply outward pressureuntil the desiredextensionis reached'
,'
"arefrlly
the leverextendslessthan 1.S mm be'
c. The probe will not remainin the UP position if
i
yond the sleeve.(SeeStep 4 above)
:
Fibrometer
Temperature'
Block
Heat
the
,2. Measuring
t
A. Methodl:
@
and place the FibroTubs Cup into
1) pipette 0.5 ml of water into a FibroTube Cup
the reaction well of the Fibrometer'
i
2l Allow at leastten minutes for temperaturestabilization'
FlllLtYv
€aL
reed\
L
andpotentiometelly-p-t
copperconstantthermocouple
useOa ttrrl/
#30 evl,F
3) \J)tr
T,:1',1t^:Iit"t
a comp:Yl9t:::tf:i:lilill;
in C andhavins
(preferably
\one
r r r E calibrated
lPlttllilolJry
wire neaqthe hot iunctionon
wind a smallcoil (6.4 mm longl of the thirmocouple
cup'
i
,
a 3.2 mm mandreland insertinto the FibroTube
4)A||owfiveminutesforstabiIization,thenreadthetemperaturN.
i
;
B r ' t h o dl l :
and pracethe cyp into the reaction
1) pipette0.b mr of waterinto a FibroTubecup
T
well.
,
72
,
r
i
,
2l Placea snrallrnercurytherrnonreterin the cup (preferablya rectal thermometerfor
babies)
3 ) A l l o w f i v e m i n u t e sf o r s t a b i l i z a t i o nt ,h e n r e a dt h e t e m p e r a t u r e .
'
T h e r e a d i n gs h o u l dd e 9 8 . 6 I 1 . 8 F | 3 7 . 2 t 0 . 5 C ) .
NOTE: A thermistor type thermometeror a largeglassthermometercannot be usbd.They
' w i l l c o n d u c th e a t t o o r a p i d l yf r o m t h e s m a l lm a s s l i q u i d
of
a n d y i e l d a f a l s el o w
reading.
C. A faulty thermostatcan be replacedby BBL, or you may order a new thermostat(BBL
CatalogNumber 70585) and the requiredphet'rolicsleeve(BBL CatalogNumber 70609).
3.MeasuringtheHeatB|ocktemperature.ThermatPrepB|ock
Follow the steps.inProcedure#2. Usea shallowwell of the Prep B lock (Note: The center
'.,
wgll in the seconiJrow of deepwells will be slightlywarmer than the others).
lf you wish to order a new thermostatfor the Prep Block, order BBL CatalogNumber
76018. lf the thermostatis not faulty and testsshow a new heat pad is needed,order BBL
Catalog
Number76015.
l
'
'
- Fibrometeror PrepB lock
4. Extremeoverheating
A. Makesuretheseinstruments
lf this is not
arenot on the samebenchwith a centrifuge.
(available
possible,
insertTWO layersof lsomodepad
from mostscientificsupplyhouses)
underthe FibroSystem@
and
instruments.
Let the machines
cool to roomtemperature
I
attemptoperationthe nextday.
B. Checkthermostatsasdescribedin Procedure
#3
#2 lFibrometer)or Procedure
( P r e pB l o c k ) .
i
,
5. The Fibrometerdoesnot stopaftera clot hasformed(doesnot detectan endpoint).
A. Dispense
0.5 ml of reagentinto a FibroTubeCup and placethe cup into thp reaction
' well.
B. Start the Fibrometerandallowthe probeto {rop.
C. Dueto the excessof liquid in the FibroTubeCrp, the Fibrometsr*rould stop immeI
:
diatelyor within 0.5 secondafterthe probehasdropped.
They shouldbe parallelto the body and
for misalignment.
D. Checkthe electrodes
reasonably
straight.Handalignmentis all that sttouldbe necessry.
t
lf
E
lf, in step C, the Fibrometer does not stop, check the cam for dirt accumqlationand
.
check for dirty electrodes.
1) Cleanelectrodeswith distilledwater. Dry with lintlesspaper.
i
2l To clean the cam, seeProcedure#6 below
the FibrometerCamAssembly
6. .Cleaning
A. Removeprobe
B. Moistena well-compactdnruabwith 7V/oisopropylalcoholand lowerth? swagthrough
the probeholeuntil it restson the top cam.
,
:
allowthecamto rotate.Movethe swabbacRandforthto
C. ActivatetheFibrometerand
with alcohol
moistened
cleanthe solidportionof the cam.Repealwith freshsryabs
I
'
Test'
Time
Prothrombin
a
with
Test
until all dirt is removed.
73
D. lf the cam is found to be defective,sendthe Fibrometerto BBL for factoryrepair,or
replacewith BBL CatalogNumber76010,Cam Assembly.
call the BBL Hot Line
in its operations,
satisfactory
E. lf stepC failsandthe camappears
(301)
(800)638-8663;
666-0100collect.
call
in Maryland
7. ElectrodeLengthAdiustments
afterthe probedrops.
A. Adjustmentsfor shorttimes:Fibrometerstops0.5 to 3.0 seconds
testwith an empty FibroTubeCup. lf the machine
NOTE: Beforemakingany adjustments,
lf
tup andtest (useonly the 0.2 ml of reagent).
in
the
put
reagent
of
0.2
ml
runs,
the
eitherof
run
on
it
not
lf
does
adjustments.
the Fibrometerruns,proceedwith
abovetests,call BBL.
'Remove
the ProbeassemblYand
hold it in your left hand so that
the black cap is PointinguPwards.
S e eF i g u r eB - 1 .
2l With your right hand, grab the
probe foot (the heavYwire extending down from the baseof
the probe) and Pull down with
0 . 9 t o 1 . 4 K g .o f P u l l .
3) Watch the hook end of the moving
electrodeas You Pull down on the
probe foot. The moving electrode
will now be shortenedbY Your
p u l l i n ga c t i o n .T h i s s h o u l ds o l v e
most short timing Problems.
4l Check electrodelengthsbY TWO
1)
methods:
a) RePlacethe Probe.DisPense
0.3 ml of reagentonly into a
FibroTubeCuP. Placea cup
into the reactionwell' With
the Fibrometor switch in the
ON Position,dePressthe Timer bar to activatethe machine'
lf the Probedoes not droP
and the detectionand timing
mechanisrnsdo not begin to
function, repeatstePs1 and 2'
lf this does not correct the
Line
Problem,call BBL, Hot
:
:
(800) 63&8663.
b) Performa final checkbY run- ning a normalcontroland an
abnormalcontrol to be zure
the correctend Pointsaredetected.
I
74
Figure8-1
B. Adjustmentsfor no eld poi!_tor for considerably
longerthan correctend point.
l ) To lengthenthe electrode,remove the probe and'hold it in
your left hand with the black cap
to the ceiling and the electrodes
facingyou.
2l Placeyour right forefingeron
top of the right angleformed by
the moving electrode.See
F i g u r e8 - 2 .
3 ) Placeyour right thumb on the
bottom of the probe foot and
a p p l y a p p r o x i m a t e l y1 . 0 K g . o f
upward pressure.This should
lengthenthe moving electrode.
4l Test the electrode length by allowing the probe to drop into 0.3 ml
reagentonly. lf the machinestops,
perform the shorteningprocedure
outlined above.Perform a normal
and an abnormalcontrol to be
surethat the correct end points
are detected.lf the problem cont i n u e s ,c a l l t h e B B L H o t L i n e .
F i gure8-2
8., Correct Protimes,will not detect PTT's. SeeProcedure#5.
9. LongPTT and Long PT.
, Problemis a dirty cam. SeeProcedure#6.
1 0 . DigitRepetition
O n o c c a s i o na, m a c h i n ew i l l d e v e l o pa c o n s t a n td i s p l a yo f o n e o f a p a i r o f b i g i t ss e p a r a t e d
by 0.5 second(0.2 and 0.7, 0.3 and 0.8 or 0.4 and 0.9). Even though this stateof constant
display does not causeresultsto go beyond the stated accuracyof the machine,many usors
f ind this condition to be undesirable.The problern must be cdrrectedat the factory. lt
is unpre
should be noted that the length of time a correctedcounter will remain ot'erative
'
I
dictable.For more detail, call TechnicalServicesat BBL.
I
't
1. StickyTirper
!
for several
lf your Fibrometerhasastickytimer,callBBL for a loaner.Hold this loaqrer
outlinedbelowhascorrectd the problem.The prob
daysuntil you arezurethe procedure
l e m i s c a u s e dm o s t o f t e n b y a p i e c eo f p l a s t i cf l a s hf r o m o n e g e a rb r e a k i n go f f a n d w e d g i n g
i n t o a n o t f r e rg e a r .
A . R e m u v et h e p f u b e a n d a c t i v a t et h e m a c h i n e .
B. Allow the machineto run for two hours.Punch the resetbutton every fifteen to twenty
m i n u t e sd u r i n gt h i s r u n n i n gt i m e . T h i s m a y c a u s et h e b i t o f p l a s t i ct h a t i s c a u s i n gt h e
j a m m i n gt o d r o p o u t .
C . l f t h i s f a i l s ,o r i s o n l y t e m p o r a r yc, a l l t h e B B L H o t L i n e .
D. lf your own inspectionsandtestss?rowthat a new counter is required,order BBL Catsl
Number 70624 and the requiredhardwarenoted in the parts list.
1 2 . F a u l t y p o w e r c o n n e c t i o n- F i b r o m e t e ra n d P r e pB l o c k ( M o d u l a r ) .
l f t h e T h e r m a lP r e p B l o c k w i l l n o t h e a t a n d t h e s w i t c h i s i n t h e O N p o s i t i o n :
A. Removethe Fibrometerline cord from the wall receptacle.
B. Separatethe Prep Block from the Fibrometer.
C . P t u g t h e f e m a l el i n e c o r d p l u g d i r e c t l y i n t o t h e P r e p B l o c k . P l u gt h e l i n e c o r d i n t o t h d
wall receptacle.
l) lf the Prep Block works, check the male plug on the Fibrometer.lf defectivd,hdv{
BBL repairit. lf the Fibrometerwas also not receivingpower initially, thert th€
femalereceptaclemust also be checked.
2l lf the prep Block stilldoes not function, check the femalereceptacle.lf defective,
haveBBL repair,or order BBL CatalogNumber 76014, FemalePlug assembly'
,,
Automatic PiPettes
Repairis not done by BBL. Defectivepipettesare replaced.Seethe repairpolicy.
:
following
The userwill find that most problemswith the Automatic Pipettecan be solvedby
the directionsgivenin SectionSevenof this manual.
.l;
76
'
i
REPLACEMENT
PARTSLIST
76017
+
?
il
7
-_rw
76015
ts
:=+
76016
{,
rt
* i
Il
I
I
i
i
ReplacementParts List: T H E R M A LP R E PB L O C K ( 6 0 4 1 9 }
rr
CATA\OG NUMBER
DESCRIPTION
76013
76014
76015
76016
76017
7 6 0 1I
Bottom CoverAsembly
FemalePlugAssembly
HeatPadand Lug Assembly
MalePlugAssembly
PrintedCircuitBoardAssembly
Thermostat
and Lug Assembly
77
fr
REPLACEMENT
PARTSLIST
\w4
*:--=+-/
it
70605
?ss I
o
f70028
5l
Repl*ement ParB List: Fibrometer@(6041
CATALOGNUMBER
DESCRIPTION
60588
70585
A L S OO R D E R :7 0 6 @
70605
70615
7ffi24
A L S OO R D E R :
70669
70691
70694
70910
7091I
70639
7W28
Line Cord
Fibromoter@
Thermostat
ThermostatPhenolic Sleeve
Light,Indicator
Time Delay,30 mfd
Capacitor,
Counter,VeederRoot
Counter,Trim Plate
Counter,Shim
Counter,Spacer
Screw-#+4OxVz"
Wastrer#4
Fuse1 ampere
Button, Reset
78
REPLACEMENT
PARTSLIST
ht_
76007
#
7600s
76012
76006
ffi
78ffi
700tr I
t
rr:;x{FtF
I
tE*o
J93
Replaoement
Par$ List: Fibrometsro(6041
5)
CATALOGNUMBER
DESCRIPTION
76000
7600r
76A02
76003
76004
76005
76006
76007
76008
76009
76010
7 6 0 11
76012
FuseClip andTie Lug
FuseClip andWireAssembly
Bottom CoverAssembly
Front CoverAssembly
RelayandSwitchAssembly
PrintedCircuitBoardAssembly- TAR
PrintedCircuitBoardAssembly- #6
SolenoidAssembly
Motor Assembly,60 cycle
ReedSwitchAssembly' :
CamAssembly
BearingBracketAssembly
HeatBlockAssembly
79
SCHEMATID
C I A G R A M- F I B R O M E T E R O
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SECTION
NINE
W A R R A N T YA N D R E P A I RP O L I C Y
87
t
-
RepairPolicy
Fibrometer@
andThermalPrepBlock
1. Any machinethat cannotbe repairedasdirectedby the Trouble- ShootingSection (SectionEight)shouldbe returnedto BBL for repair.A loanermachineis
usuallyavailable
for immediate
sfripment
on request.
Callthe toll-freenumber
(800) 638-8663to arrangefor shipmentof a loanermachineand repairof your
machine.BBL will allowone monthfrom the time the nrachineis shippeduntil
receiptof the customer'sunit for repair.BBLlruillalsoallowone month after
shipmentof the repairedunit for returnof the loaner,Failureto meetthese
datesmay resultin a delayin shippinga loanerto you whenyou noedit. These
loanerFibrometerTimersand ThermalPrepBlocksarenot for sale.
provided,of
2. Please
do not shipyour unit to BBL until the loaneris received,
course,that a loanerwasnecessry.CallTechnicalServices
at BBL, (8001
638-8663,to determineif a loaneris needed.The loanerunit will havewith it
and a peel-stickBBL address
a defectiveunit returnauthorization,
label.Please
checkoff the list of possibledefectsany which you may haveexperienced.
Add
any note you feel may be helpfulin repairingthe unit. Packyour Fibrometsrin
the loanerbox with the defectiv*unitreturnauthorization.
Sealthe box and
placethe BBL address
labelon it.
slip,
3. Do not shipby ParcelPostunlessyou insureit. Withoutthe insurance
if
it
is
lost.
thereis no chanceof tracingthe shipment
Automatic Pipettes
2
them. Referto the
BBL doesnot repairdefectiveAutomaticPipettes;it exchanges
to you. The
unit will be airshipped
under"Warranty".An exchange
discussion
defectiveunit must be returnedso that it canbe repairedandplacedin the exchangepool.
90
REFERENCES
'
1. Fewell,R. G.,A. Cundy,andG. C. Jenkins,1g72.TheOneStageprothrombin
Test:A
Comparative
Studyof Mechanized
Prothrombinmeter
and ManualTesting.Med.Lab.
T e c h 2. 9 : 1 4 7 .
2' Miale,J. B. 1965.The FibrometerSystem
of RoutineCoagulationTests.
Amer.J. Clin.
Pathol.43:475.
.
3. Sterling,
R. E.,A. A. Wilcox,A. G. Ware,andM. K. Umehara.
1965.lmprovedInstrumentationfor the Determination
of Prothrombin
Activity.Clin.Chem.I l:4Og.
4. Barnett,R. N., andC. L. Pinto.1966.Reproducibility
of Prothrombin
TimeDeterminationsBetweenTechnologists.
Am J. crin. pathol.46:14g.
5. ViralHepatitis.
1973.WorldHealthOrganization.
WHO Tech.Rep.Serv.
#512. pageB2O.
91