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CRYOPR
RESERVA
ATION OF
O CELL
LS USIN
NG OriCe
ellTM
CRYOPR
RESERVA
ATION MEDIA
M
R Protein-F
Free Cryoprreservation Medium (C
Cat. No. NC
CPF-10001) is a
OriCellTM NCR
use freezing
g medium. Its chemically-define
ed and prottein-free
prrotein-free,, ready-to-u
fo
ormulation has
h been optimized to
o stem cells
s and prima
ary cells, th
hus greatly enhancing
he viability and integrity of these cells by prrotecting th
hem from da
amage durring the
th
on
ne-step free
p
Unlike other conventiional freezi ng media, which
eze-thaw procedure.
re
equire a slow programmed freeze
e, this prod
duct allows the cells to
o be directly
y frozen at
-8
80°C.
Cryopreservation
ge the cultu
ure medium
m with fresh
h growth medium
m
24 h
hours before freezing
N
Note: Chang
g.
1.
Collect ce
ells that are
e in the log
garithmic growth phas
se. Perform
m a cell count to
determin
ne the viable cell dens ity.
2.
Centrifug
ge the cells for 3-5 mi nutes at 25
50 x g and 20°C. Rem
move and discard the
supernattant using a pipette.
3.
Resuspen
nd the cell pellet in th e OriCellTM NCR Protein-Free Cry
yopreservation Medium
m
at a cell d
1 5-106 cellls/mL.
density of 10
4.
Dispense
e aliquots of the cell su
uspension into cryogenic storage
e vials that are
properly labeled.
5.
Place the
e vials direc
ctly in a -80
0°C freezerr. After 24 hours, tran
nsfer the fro
ozen vials
to liquid nitrogen fo
or long-term
m preservattion.
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