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23. Add 75 µL DEPC-Treated ddH2O onto center of membrane in each well of the plate. Centrifuge at 4000 x g for 4 minutes at room temperature. 24. Repeat the elution step (step 23) once with second 75 µL DEPC Treated ddH2O.Seal the plate contains purified RNA and store at 70℃ Page 10 Biomiga EZgeneTM Tissue RNA Kit Protocol