1
Download Sample Data Sheet
Transcript
RapidSeqTM High Yield Small RNA Sample Prep Kit 0512V5 Description Components in this kit are prepared with pure chemic als to construct NGS libraries compatible with Illumina’s sequencing plat form for subsequent cluster generation, using purified small RNA or total RNA contains small RNA fragments as input. Four sets of the kit with different 4 sets of 12 aligners, respectively, are available. Quality Control At least one kit of each lot has been tested for small RNA NGS library construction using Adult Normal Lung Tissue Total RNA (Cat # R1234152-50) and Illumina’s NGS instrument. Good coverage and low adapter dimmer are observed. Components One kit with aligner has 3 boxes listed in below; only one aligner box is included in one kit. (see table 2-4 below). Reagents are sufficient for 12 assays. The kit without aligner only includes 2 boxes. Table 2. Contents List of RapidSeq Cap Color Green Red Yellow Blue Amber TM High Yield Small RNA Sample Prep Kit (Box 1 of 3) Item Tail Oligo Tail Buffer Tail Enzyme Ligation Enhancer Cap Oligo Cap Enzyme RT Oligo RT Enzyme Universal Primer PCR MasterMix Gel Cutting Indicator TM Table 3. Contents List of RapidSeq Item Gel Cutter Gel Breaker Gel Filter DNA Storage Solution Amount in kit 30 µ l 30 µ l 30 µ l 27 µ l 30 µ l 30 µ l 60 µ l 60 µ l 60 µ l 700 µ l 26 µ l Part No. KS074012-3 KS074012-4 KS074012-5 KS074012-6 KS074012-7 KS074012-8 KS074012-9 KS074012-10 KS074012-11 KS074012-12 L5022100-DS High Yield Small RNA Sample Prep Kit (Box 2 of 3) Amount in kit 12 12 12 1500 µ l x 2 3 Part No. KS071012-11 KS071012-12 KS071012-13 LB3401010
