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Viapath Analytics - Bedford Hospital Viapath Bedford Pathology User Guide BED-USER-14 Pathology Department Test Specimen requirements and containers Method/ Comments/ Reference laboratories Turnaround times Amikacin, tobramycin and teicoplanin 5ml clotted blood – red and yellow top. Samples should be taken pre dose and 1 hour post dose for most agents. Please contact the Consultant Microbiologist for further advice. Samples referred to Antimicrobial Reference Laboratory, Department of Medical Microbiology, Southmead Hospital, Bristol, BS10 5NB Ascitic and Peritoneal Dialysis fluid Aspirates and fluids form normally sterile sites, pus, tissues 20ml maximum in sterile universal Contact Microbiology if urgent microscopy is required. 20ml maximum in sterile universal No formalin Contact Microbiology if urgent microscopy is required. Aspirates and fluids from normally sterile sites, pus, tissues for acid fast bacilli (AFB) Blood cultures 20ml maximum in sterile universal No formalin AFB isolates are referred to the National Mycobacterium Reference Laboratory, Abernethy Building, Institute of Cell and Molecular Science, 2 Newark Street, London, E1 2AT Remove the centre portion of the barcode and place on the request form. Blood culture bottle sets available from Pathology Out of hours place blood culture bottles in the incubator located rd on the 3 floor of Pathology. Amikacin and tobramycin available next day if received before 9.30am Teicoplanin next working day (Verbal results) Microscopy same day, culture 2-3 days. Microscopy same day, culture 2 -3days (may be extended when appropriate) Microscopy 1-2 working days Culture 6 – 8 weeks Aerobic bottles 8 – 10ml Anaerobic bottles 8 – 10ml Paediatric bottles 1 – 3ml 48 hour interim report, 5 day final, 7 days for suspected bacterial endocarditis Please provide relevant clinical information including differential diagnosis, appropriate travel history, possible contact with infectious disease or predisposing condition. Samples are incubated using the BD BacTec system Bronchial aspirate/trap for routine culture Sealed trap or Sterile universal Bronchial aspirate/trap for acid Sealed trap or Sterile universal File Name: BED-USER-14 Viapath Bedford Pathology User Guide Author: A. Strong Authorised: G. Nicholson TM FX blood culture 2-3 days Processing of isolates is performed using the Bactec MGIT 960. Microscopy 1-2 working days Version: 5.5 Issue Date: 30/04/2015 Page 69 of 99
