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blastncbi Description MatrixValue Property to select a substitution matrix for amino acid sequences. Enter 'PAM30’, 'PAM70', 'BLOSUM80', 'BLOSUM62', or 'BLOSUM45’. The default value is 'BLOSUM62'. InclusionValue Property for PCI-BLAST searches to define the statistical significance threshold. The default value is 0.005. PctValue Property to select the percent identity. Enter None, 99, 98, 95, 90, 85, 80, 75, or 60. Match and mismatch scores are automatically selected. The default value is 99 (99, 1, -3) The Basic Local Alignment Search Tool (BLAST) offers a fast and powerful comparative analysis of interesting protein and nucleotide sequences against known structures in existing online databases. blastncbi(Seq, Program) sends a BLAST request against a sequence (Seq) to NCBI using a specified program (Program). • With no output arguments, blastncbi returns a command window link to the actual NCBI report. • A call with one output argument returns the Report ID (RID) • A call with two output arguments returns both the RID and the Request Time Of Execution (RTOE, an estimate of the time until completion) blastncbi uses the NCBI default values for the optional arguments: 'nr' for the database, 'L' for the filter, and '10' for the expectation threshold. The default values for the remaining optional arguments depend on which program is used. For help in selecting an appropriate BLAST program, visit http://www.ncbi.nlm.nih.gov/BLAST/producttable.shtml Information for all of the optional parameters can be found at 6-25