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Table 6. The mutation detection efficacy of the softwares.
SeqScape
Lasergene
Sequencher
SNP
5
3
5
Homozygous Mutation
5
4
3
Heterozygous Mutation
5
3
2
Overall
15
10
10
The above evaluation ranks the software’s ability to detect mutations in a scale of 5, with
5 representing the highest score. SeqScape scored the highest as it is capable of
recognizing all three types of mutation. Lasergene and Sequencher both achieved
equivalent scores. Lasergene is not the ideal software to detect the identity of the SNP as
well as mutation in one allele. Sequencher is not the ideal tool to identify frameshift
mutations in general.
Table 7. The ease of use of the softwares.
SeqScape
Lasergene
Sequencher
Appearance
3
4
5
Setup Procedures
4
3
4
Alignment features
5
2
3
12
9
12
- Select Reverse Complement
- Work with Poor Quality Data
- Trimming Options
Overall
The above evaluation looks at the ease of use of the softwares in a scale of 5, with 5
representing the highest score. Both SeqScape and Sequencher scored the highest overall
score for its well-designed layout as well as the ease of use.
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