Download MagCore® Viral Nucleic Acid Extraction Kit

User Manual
MagCore
HF16
MagCore
MagCore® Viral Nucleic Acid Extraction Kit
For extracting Viral DNA/RNA from serum, plasma, cell-free body fluids
by using the MagCore® HF16 System of RBC Bioscience Corp.
Cat. No. MVN400-01 // MVN400-02
Cartridge Code:
201
ASADADADADADAD
MAC1601-98001
MagCore® Viral Nucleic Acid Extraction Kit
MagCore HF16 Automated DNA/RNA Purification System
MagCore® HF16 System
Reliable Nucleic Acid Purification
MagCore HF16 System is a simple, fast and cost-effective technique to automatically
purify nucleic acids from a diverse range of sample sources. With the pre-programmed
protocols and magnetic bead based reagent cartridges the system provides consistent
and stable nucleic acid purification for every busy laboratory.
Advantage
Touch Button operation means simplicity
Rapid Results in about 30 minutes for DNA purification
Flexible Design means single samples can be run with no waste
Batch Purify up to 16 samples at one time
Diverse Purification kits and protocols available
Economic Pricing of reagents and instrument
1
MagCore® Viral Nucleic Acid Extraction Kit
MagCore HF16 Introduction
1
MagCore
HF16
2
3
4
UV
Shift
Stop Esc
MagCore
Enter
Start
5
6
7
8
1. Indicator LCD
2. LCM Display Screen
3. UV Key
4. Aluminum Hinge
5. Keypress Panel
6. Protective Acryl Board
7. Door Pull
8. Power Switch
9. UV Lamp
10. Door-Open Detector
11. Door Pry
12. “ MagCore” LCD Window
13. Syringe Unit
14. Operation Area
Instrument Supplied Accessories
Catridge Rack X 2
9
T-Rack X 2
10
11
Empty Cartridge X 20
MagCore
12
13
14
Tip Holder X 50
Tip X 50
Sample/Elution Tube X 50
2
MagCore® Viral Nucleic Acid Extraction Kit
MagCore HF16 Specification
HF16
Model
Processing Capacity
16 samples per batch
Programs built-in.
Whole Blood (200/400ul), Viral Nucleic Acid (DNA/RNA), Tissue Genomic DNA, Plant Genomic DNA
Cellulose coated magnetic beads
200/400/1,200μl (2,400μl available on request with optional 2.4ml Rack)
60/100/150/200 μl
Avrage 6μg Genomic DNA / 200μl human whole blood
O.D. 1.8-2.0 A260/280 ratio
30-70 minutes (depends on sample type and method)
1. Pipetting Unit: Dispensing, transferring, X-Z two axis movements.
2. Electric Control: Internal microprocessor
3. UV Light: Power 5W, life duration >1,000 Hrs
4. Heating Block: RT~1000C
5. Display Screen: 3 inches LCM Screen with keypress Panel
6. Accessories: T- Rack, Cartridge Rack , Empty Cartridges, Tip/Holders , Microcentrifugr Tube, Syringe O-ring and Grease
Voltage: AC 100-240V; Frequency: 50/60Hz; Power Consumption >1.0KVA
W550 X D660 X H680 (mm) / W21.65 X D25.98 X H26.77 (inches)
70kg / 155lbs
Protocols V1.01
System Method
Sample Volume
Elution Volume
Yield
Purity
Processing Time
System Components
Power Supply
Dimension
Net Weight
LCM Display Screen and Keypress Panel
UV
Shift
Stop
Esc
Enter
Start
MagCore HF16 provides a touch button keypree panel for you to
communicate with your instrument. The number keys give users
to input Catridge Code which specified on the Reagent Cartridge
to run built-in program automatically, or to select desired
volumes at start-up sample and final elution steps.
LCM Display Screen which with sapphire background, shows clear
information that the MagCore HF16 is currently performing.
Special function of buttons described as:
UV
Shift
Stop
UV button is designed to turn on UV Lamp while your MagCore HF16 needs
to be sterilized after runing contagious samples. Options with 30 / 60 mins.
Shift button is designed for calibrating position accuracy at MagCore HF16
System, DO NOT excute this program without trained engineer aside.
Stop button is designed for going back to “Stand-By” menu at program
completed, or for EMERGENCY STOP use.
3
MagCore® Viral Nucleic Acid Extraction Kit
Operation Procedures
2
1
MagCore
UV
HF16
Shift
Stop Esc
Enter
MagCore
Start
Turn on power switch
located at the right hand
side of MagCore HF16. A
beep sound can be heard
and the machine is on
“Stand-By” condition.
MAGCORE HF16
STAND-BY
LCM display screen shows
“ Stand-By” as left picture.
PRESS <START> TO RUN
Note:
At this step, press UV button,
to perform UV decontamination
3
Initializing
After press Start button,
machine runs program of
calibration, initialize to
move all axis to original
factory positions.
4
INPUT CARTRIDGE CODE
(3 DIGITALS)
<XXX>
Input Catridge Code : 201
to run Viral Nucleic Acid
(DNA/RNA) protocol.
Catridge Code is shown on
your Reagent Cartridge and
the cover of user manual.
5
INPUT CARTRIDGE CODE
(3 DIGITALS)
(201)
<ENTER> NEXT PAGE
CARTRIDGE CODE : 201
VIRAL DNA / RNA 400
<ENTER> NEXT PAGE
<ESC> CANCEL
CARTRIDGE CODE : 201
VIRAL DNA / RNA 200
<ENTER> TO NEXT PAGE
<ESC> CANCEL
4
Confirm your input code
again and press Enter to
next page for sample
volume selection.
Press 1 for 400µl sample 7
Press Enter to next page
Press Esc to cancel whole
procedures and back to
“Stand -By” menu.
Press 2 for 200µl sample
Press Enter to next page
Press Esc to cancel whole
procedures and back to
“Stand -By” menu.
6
VIRAL NA 400/200
SELECT SAMPLE VOLUME
<1> 400µl
<2> 200µl
<ESC> PREV PAGE
Viral Nucleic Acid 400/200
protocols instaled.
Press 1 for 400µl sample
Press 2 for 200µl sample
Press Esc to re-enter cartridge
code
8
ENSURE RACKS LOADED
(1) CARTRIDGE RACK
(2) T- RACK
<ENTER> NEXT PAGE
At this step prepare racks to
operation area.
(see Step 9-18)
After racks loaded then press
Enter to next page for
elution volume selection.
MagCore® Viral Nucleic Acid Extraction Kit
Operation Procedures
10
9
A. Slide MagCore cartridge
into Cartridge Rack to the
end with slight downward
angle.
Insert MagCore Viral Nucleic
Acid cartridge into Cartridge
Rack following arrow point
directions.
B. Press down the cartridge
to interlock position under
the over-hang part of
Cartidge Rack. A click sound
can be heard when it is
locked correctly.
11
Load as many cartridges
as sample runs. Capacity
for each Cartridge Rack is
8, up to 16 samples for two
Cartridge Racks can be
performed at one time on
MagCore- HF16.
12
INPUT CARTRIDGE CODE
(3 DIGITALS)
<XXX>
13
Instal the cartridges loaded
Cartridge Rack into inner
operation area of the
MagCore HF16 and lock
firmly.
14
Hole 4
Put Tips into Tip Holders.
Set the Tip/Tip Holder onto
hole 2 of T-Rack
Hole 1
15
Put a microcentrifuge tube
onto hole 1 of T-Rack as
Elution Tube.
Elution Tube
A. Dissolve the Carrier RNA
2mg with 1ml of provided
RNase-Free Water.
16
Sample Tube
B. Pipet 200/400µl serum
sample to a microcentrifuge
tube, then add 5µl of Carrier
RNA solution to per 200µl of
serum sample.
C. Put the Sample Tube into
hole 4 of T-Rack.
Note:
Store the Carrier RNA at 40C for long term storage.
5
MagCore® Viral Nucleic Acid Extraction Kit
Operation Procedures
18
17
Pull down the front door
to interlock position and
then press Enter to next
page for elution volume
selection.
Instal the Tip/Holder and
Sample/Elution Tubes
loaded T-Rack into outter
operation area of the
MagCore HF16 and lock
firmly.
19
Select final elution volume
<1> 200µl
<2> 150µl
<3> 100µl
<4> 60µl
SELECT ELUTE VOL.
<1> 200 µl
<2> 150 µl
<3> 100 µl
<4> 60 µl
<ESC> PREV PAGE
20
PREHEATING...
Tmp= 045C
MagCore HF16 in process of
selected protocol at this
step. The Green Indicat LCD
lights up and Heating Block
starts to heat up to 600C for
Lysis Step.
PROCEDURE COMPLETED
While program finished, a
beep sound can be heard.
and green Indicate LCD
light went out.
ELUTE VOLUME: 200µl
21
MagCore
HF16
UV
Magnetic Separation
Shift
Stop Esc
MagCore
Enter
Start
During MagCore HF16 is
under program running,
the “MagCore” LCD lights
up at all times.
DO NOT open the door at
this moment, it causes
emergent stop and you
might lose your samples by
machine interruption.
22
<START> CONTINUE
<STOP> STAND-BY
LCM displays as left picutre.
24
23
Lift up the front door to the
uppermost position, and take
out Elution Tube with purified
viral DNA/RNA, direct for
downstram applications in
PCR and RT-PCR, or store at
-200C for long term stroage.
Discard all disposables under
proper procedures.
6
PROCEDURE COMPLETED
<START> CONTINUE
<STOP> STAND-BY
After clean up disposables.
you can choose to
Start: run the same protocol
again
Stop: to “Stand-By” menu
for UV sterilization or
power shut down.
MagCore® Viral Nucleic Acid Extraction Kit
UV Sterilization
2
1
MagCore
Turn on power switch
located at the right hand
side of MagCore HF16. A
beep sound can be heard
and the machine is on
“Stand-By” condition.
UV
HF16
MAGCORE HF16
STAND-BY
PRESS <START> TO RUN
Shift
Stop Esc
Enter
Start
MagCore
UV LIGHT
<1> 1 hr
<2> 30min
LCM display screen shows
“ Stand-By” menu as left
picture.
MAGCORE HF16
STAND-BY
PRESS <START> TO RUN
3
4
Press UV button to proceed
UV sterilization with 30/60
mins of time options.
UV lamp actives and count
down with selected time at
minutes.
UV Lamp Actived !!!
DO NOT OPEN DOOR
059 Min / 60 Min
<ESC> To Back
MagCore HF16 will go
back to “ Stand-By” menu
once UV sterilization
procedure completed.
Esc: to “Stand-By” menu
Door-Open Interruption / Emergent Stop
2
1
If the front door was opened
during program performing,
MagCore HF16 stops all
mechanical movements
immediately due to safty
consideration.
EMERGENT STOP!!
<START> CONTINUE PROGRAM
<STOP> ABORT
LCM display screen shows
warning sign, Red Indicate
LCD lights up and a beep
sound can be heard.
Pull down the front door, and select
Start : continue program.
Stop : abort program and back to Stand-By” menu.
4
3
MagCore
HF16
UV
UV
Shift
Stop Esc
Enter
Start
After machnical dysfuntion
eliminated, select
Shift
Stop
Esc
Enter
Start
MagCore
Start : continue program.
Stop : abort program and
back to “Stand-By”
menu.
In case of emergency, press “STOP” button at anytime.
LCM display screen shows warning sign, Red Indicate
LCD lights up and a beep sound can be heard.
7
Kit Contents
MVN400-02
MVN400-01
Pre-filled Cartridge Reagent
36
Pre-filled Cartridge Reagent
96
Pipette Tip
36
Pipette Tip
100
Tip Holder
36
Tip Holder
100
Microcentrifuge Tube
72
Microcentrifuge Tube
200
Tube Cap
36
Tube Cap
100
Carrier RNA (2mg)
1
Carrier RNA (2mg)
2
RNase-Free Water (10ml)
1
RNase-Free Water (10ml)
1
RBC Bioscience Corp.
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