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Method:....................................................................................................................................20
Post-Synthesis Processing..............................................................................................................................20
Overview...................................................................................................................................................20
Cleanup.....................................................................................................................................................20
Materials:.................................................................................................................................20
Method:....................................................................................................................................20
Base Deprotection.....................................................................................................................................20
Materials:.................................................................................................................................21
Method:....................................................................................................................................21
Shutting Down the Arrayer.......................................................................................................................21
Control Hybridization for Quality Control....................................................................................................21
Overview...................................................................................................................................................21
Hybridization............................................................................................................................................21
Materials:.................................................................................................................................21
Post-Hybridization Washes......................................................................................................................23
Materials:.................................................................................................................................23
Scanning....................................................................................................................................................24
Materials:.................................................................................................................................25
Method:....................................................................................................................................25
Interpretation.............................................................................................................................................25
Maintenance and Troubleshooting.................................................................................................................26
Clearing Clogged Inkjet Pumps................................................................................................................26
Cleaning the Phosphoramidite and Tetrazole Supply Vials.....................................................................26
Materials:.................................................................................................................................26
Method:....................................................................................................................................26
Changing the Inkjet Head.........................................................................................................................27
Materials:.................................................................................................................................27
Method:....................................................................................................................................27
System Properties...........................................................................................................................................28
System Performance.................................................................................................................................28
Speed and Failure Rate............................................................................................................28
Spot Diameter..........................................................................................................................29
Hybridization Specificity.........................................................................................................29
Two-color Hybridization.........................................................................................................29
Cost of Operation....................................................................................................................30
Future Improvements................................................................................................................................30
Appendix A: INKJET ARRAYER STARTUP PROCEDURE CHECKLIST.............................................31
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