Download Protein Quantification Assay - MACHEREY

Protein Quantification Assay
Table of contents
1 Components 4
1.1 Kit contents
4
1.2 Consumables and equipment to be supplied by user
4
2 Product description
5
2.1 The basic principle
5
2.2 Kit specifications
5
2.3 Handling, preparation, and storage of starting materials 8
2.4 Calibration curves
9
2.5 Recommended sample volumes 2.6 Alternative wavelengths for extinction measurement 9
11
3 Storage conditions and preparation of working solutions
12
4 Safety instructions
13
5Protocols
15
5.1 Microplate assay procedure
15
5.2 Semi-microcuvette assay procedure
18
5.3 Microcuvette assay procedure
21
5.4 Low volume assay procedure
24
6Appendix
27
6.1 Guidance for data analysis – calculation of protein concentration
27
6.2Troubleshooting
30
6.3 Ordering information
31
6.4References
32
6.5 Product use restriction / warranty
32
MACHEREY-NAGEL – 05 / 2014, Rev. 04
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