Download User Manual, GeneChip® Whole Transcript WT

Chapter 1 | Overview
Table 1.2 (Continued) Necessary Reagents
Material
Source
P/N
GeneChip® WT cDNA Synthesis and Amplification Kit
Affymetrix
900673 (30 Rxn) or
900672 (10 Rxn)
Affymetrix
900671 (30 Rxn) or
900670 (10 Rxn)
Sub-kit 1: GeneChip® WT cDNA Synthesis Kit
Contains:
† T7-(N) Primers, 2.5 μg/μL
6
st
† 5X 1 Strand Buffer
† DTT, 0.1M
† dNTP, 10 mM
† RNase Inhibitor
™ II
† SuperScript
† MgCl , 1M
2
† DNA Polymerase I
† RNase H
† Random Primers, 3 μg/μL
† dNTP+dUTP, 10 mM
† RNase-free Water
Sub-kit 2: GeneChip® WT cDNA Amplification Kit
Contains:
† 10X IVT Buffer
† IVT NTP Mix
† IVT Enzyme Mix
† IVT Control
Fragmentation and Labeling
GeneChip® WT Terminal Labeling Kit
Contains:
† 10X cDNA Fragmentation Buffer
† UDG, 10 U/μL
† APE 1, 1,000 U/μL
† 5X TdT Buffer
† TdT, 30 U/μL
† DNA Labeling Reagent, 5 mM
† RNase-free Water
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