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11.3. INSERT INTO VECTOR
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Figure 11.2: Digest into fragments options dialog, with extended options showing.
11.3
Insert into Vector
The option Insert into Vector... from the Tools / Restriction Analysis menu or the context menu
allows you to take an insert and insert it into a vector. The insert must be one of the following:
• A fragment which has already been digested. This fragment cannot have any restriction
site annotations on it. The entire fragment will be inserted into the vector. Overhangs
will be taken into account.
• A sequence with two restriction annotations. The fragment resulting from digesting this
sequence (and discarding the fragments from the ends) will be inserted into the vector.
The vector must be a circular sequence. You do not need to annotate the restriction sites used
to cut the vector in advance; the Insert into Vector operation will do that for you.
This operation cannot deal with some aspects of molecular cloning such as triple ligation and
the blunting or filling in of overhangs. If you want to do a cloning operation outside the scope
of this operation, you will need to annotate restriction sites on the sequences involved, digest
the fragments, modify them in the sequence viewer if necessary and then ligate them back
together as a set of discrete steps.