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6. Description
The ECL Plex Western blotting system is optimized for single protein
detection as well as multiplex protein detection using ECL Plex CyDye
conjugated secondary antibodies and Hybond-LFP (low fluorescent
PVDF membrane), or Hybond-ECL (nitrocellulose membrane).
The optimized antibodies show high sensitivity, a very good dynamic
range and low or no cross reactivity. The CyDye technology is safe
and reliable and ensures accurate quantification. The system is
optimized on the Typhoon scanner but is compatible with many
fluorescent laser scanners and CCD cameras (see section 7).
The ECL Plex Fluorescent Rainbow markers is a mixture of individually
colored proteins of defined size. Purified proteins are combined
to produce bands of equal color intensity and even spacing when
separated on a polyacrylamide gel. The visibility of the individual
marker bands in the Cy3 or Cy5 can vary and depend on the primary
antibodies used.
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