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* This blocker can reduce unspecific detection ++++ = high performance +++ = good performance ++ = acceptable performance + = poor performance - = not compatible Ratings are based on overall performance including level of auto fluorescence/background and unspecific detection as well signal intensity 7.3. Fluorescent imaging systems Imaging systems with the capability of detecting Cy2, Cy3 and Cy5 are compatible with the ECL Plex Western blotting system; however, the ECL Plex CyDye coupled secondary antibodies have been optimized using Typhoon, Ettan™ DIGE and Storm™ Imagers. Performance may vary depending on the imager used (see Table 2). 16