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Introduction E-Gel® Electrophoresis System Introduction The E-Gel® agarose gel electrophoresis system is a complete bufferless system for agarose gel electrophoresis of DNA samples. The major components of the system are: • E-Gel® pre-cast agarose gels • Electrophoresis bases E-Gel® pre-cast agarose gels are self-contained gels that include electrodes packaged inside a dry, disposable, UV-transparent cassette. The E-Gel® agarose gels run in a specially designed device that is a base and power supply combined into one device (two bases are available for running E-Gels, the new iBase™ system and the original, economical E-Gel® Powerbase™). Advantages of E-Gel® Agarose Gels Throughput Capacity Using E-Gel® agarose gels for electrophoresis of DNA samples offer the following advantages: • Provides fast, safe, consistent, high-resolution electrophoresis • Eliminates the need to prepare agarose gels and buffers, and to stain gels • Compatible with most commercially available robotic systems for high-throughput agarose gel electrophoresis • Available in a variety of agarose percentages, well formats, and throughput capacities to suit your applications • Offered with a number of different DNA gel stains to accommodate your application • Includes E-Gel® CloneWell™ and E-Gel® SizeSelect™ gels, to accelerate and simplify DNA gel purification and improve cloning results Three categories of E-Gel® agarose electrophoresis systems are available from Invitrogen based on your throughput requirements. • Low-Throughput E-Gel® Electrophoresis System designed for electrophoresis of 8–16 DNA samples per gel. • Medium-Throughput E-Gel® Electrophoresis System designed for electrophoresis of 48 DNA samples per gel. This system is compatible for use with multichannel pipettors or automated liquid handling systems. • High-Throughput E-Gel® Electrophoresis System is designed for electrophoresis of 96 DNA samples per gel. This system is compatible for use with multichannel pipettors or automated liquid handling systems. 1