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T3000 Manual __________________________________________________________________ 15 Temp[C] 1: 99.0 2: 99.0 3: 55.0 4: 34.0 Info Time # Options --> ¬ 0h 5m 0s 0h 1m 0s 0h 1m 0s 2 0h 1m 0s Insert/Del Save Pgm Enter In the row labelled with # you can enter the number of backloops. Note: total cycler number = (n backloops) + 1, e.g. enter 29 for a total number of 30 cycles. Temp[C] 1: 99.0 2: 99.0 3: 55.0 4: 34.0 Info Time # Options --> ¬ 0h 5m 0s 0h 1m 0s 0h 1m 0s 2 24 0h 1m 0s Insert/Del Save Pgm Enter This protocol now contains 25 cycles between step 2 (99.0°C for 1minute) and step 3 (55.0 °C for 1 minute). 4.8 Cool below ambient temperature Temp[C] 2: 99.0 3: 55.0 4: 34.0 5: 4.0 Info Time # Options --> ¬ 0h 1m 0s 0h 1m 0s 2 24 0h 1m 0s Pause Insert/Del Save Pgm Enter To hold the temperature for an indefinite time enter “0”. After pressing [Enter] “pause” is displayed. 4.9 Save program Save program with [Save Pgm]. The program data are written to the permanent memory. Temp[C] 2: 99.0 3: 55.0 4: 34.0 5: 4.0 Info Time # Options --> ¬ 0h 1m 0s 0h 1m 0s 2 24 0h 1m 0s Pause Insert/Del Save Pgm Enter Save program with [Save Pgm].