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CryoPRO Workstation User Manual Figure 6: Disposable Cartridge latched onto the Oscillating Disposable Cartridge Holder 3. Place the Mixing Chamber into the Rotating Mixing Chamber Holder so that the DMSO Filter is away from you (12 o’clock). • Figure 7: Mixing Chamber in Rotating Mixing Chamber Holder with DMSO Filter at 12 o'clock 4. Remove and retain the cap from the DMSO filter and open the clamp on the sterile connection tubing. 5. Allow the buffy coat to drain until it is approximately half empty. The fluid level should be level with the seam. Page | 20 14-02-016 Revision B