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Table of Contents Preface ........................................................................................................ 7 Chapter 1: Getting Started with Scaffold............................................... 11 Chapter 2: Identifying Proteins with Scaffold ....................................... 23 Chapter 3: Loading Data in Scaffold ...................................................... 37 Chapter 4: The Scaffold Window............................................................ 61 Chapter 5: The Load Data View ............................................................ 111 Chapter 6: The Samples View............................................................... 121 Chapter 7: Quantify View ...................................................................... 143 Chapter 8: Protein Grouping and Clustering ...................................... 151 Chapter 9: Quantitative Methods and tests......................................... 163 Chapter 10: Precursor Intensity Quantitation ..................................... 179 Chapter 11: Reports............................................................................... 189 Appendix................................................................................................. 205 Scaffold User’s Manual 5